2013 Fiscal Year Final Research Report
Possibility of bone substitute by using tissue engineering, concerning the bone grafting operation to the alveolar cleft of CL/CP.
| Project/Area Number |
23592942
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IKEMOTO Shigehiro 北里大学, 医学部, 助教 (90296492)
BABA Kyoko 北里大学, 医学部, 特別研修生 (90327411)
AOYAGI Kazuya 北里大学, 医学部, 講師 (10337959)
ISHIGURO Masashi 北里大学メディカルセンター, 医学部, 研究員 (40265640)
KUMAZAWA Kenichi 北里大学, 医学部, 助教 (60383618)
SUGIMOTO Takayuki 北里大学, 医学部, 助教 (20365133)
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| Co-Investigator(Renkei-kenkyūsha) |
OIDA Shinichiro 鶴見大学, 歯学部, 教授 (10114745)
MOCHIZIKI Junko 北里大学, 医学部, 講師 (90306613)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 未分化間葉系細胞 / 凍結保存 / 再生医工学 |
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| Research Abstract |
Bone formation of Mesenchymal stromal cells(MSCs) derived from a surplus of maxillary bone fragments, in primary cleft lip / palate operation, was confirmed in vivo. When cultured with serum-free medium, maxilla-derived MSCs exhibited osteogenic potential in both in vitro and in vivo. With bone tissue-derived MSCs cryopreserved more than 10 years, in the safety assessment, no abnormality was found in G-band patterns as a morphologic investigation and in myc or p53 analysis. Moreover osteogenic potential and multipotency were maintained. Cryopreserved bone tissue-derived MSCs may be clinically useful.
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