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2013 Fiscal Year Final Research Report

Transcriptome analysis of dental follicle cells

Research Project

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Project/Area Number 23592947
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Surgical dentistry
Research InstitutionNihon University

Principal Investigator

OGURA Naomi  日本大学, 松戸歯学部, 講師 (10152448)

Co-Investigator(Kenkyū-buntansha) ITO Ko  日本大学, 松戸歯学部, 助教 (20419758)
Research Collaborator TAKAHASHI Kosuke  日本大学, 松戸歯学部, 助教 (30705687)
Project Period (FY) 2011 – 2013
Keywords歯嚢由来細胞 / トランスクリプトーム / microRNA / microRNA標的遺伝子 / 骨芽細胞分化 / 石灰化
Research Abstract

The dental follicle, which is an ectomesenchymal tissue that surrounds developing tooth germ, contains stem cells and/or progenitor cells of the periodontium. Human dental follicle cells (hDFC) have the ability to mineralize under in vitro culture using osteogenic induction medium (OIM). hDFC have great potential for utilized in regenerative cell therapy.
MicroRNAs (miRNAs) are characterized a class of small non-cording RNAs that mediate the post-transcriptional regulation of gene expression by binding to partially complementary site 3'-untranscriptional regions of target mRNAs. miRNA have emerged as key regulators of diverse biological process.
In this study, we performed the gene expression profiles for miRNA and mRNA in hDFC during osteogenic differentiation, and analyzed the biological role of the miRNAs during osteogenic differentiation by examining the expression of bioinformatically predicted miRNA target genes.

  • Research Products

    (11 results)

All 2013 2012 2011

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (8 results)

  • [Journal Article] Bone morphogenetic protein 6 stimulates mineralization in human dental follicle cells without dexamethasone2013

    • Author(s)
      Takahashi K, Ogura N, Aonua H, Ito K, Ishigami D, Kamino Y, Kondoh T
    • Journal Title

      Arch Oral Biol

      Volume: 58 Pages: 690-698

    • DOI

      10.1016/j.archoralbio.2012.10.018

    • Peer Reviewed
  • [Journal Article] Gene Expression and Protein Production of Leukemia Inhibitory Factor in Human Dental Follicle Cells2012

    • Author(s)
      Fujimoto Y, Ogura N, Aonuma H, Takahashi K, Ito K, Kondoh T
    • Journal Title

      Int J Oral-Med Sci

      Volume: 11 Pages: 156-162

    • URL

      http://www.mascat.nihon-u.ac.jp/labo/info/index.html

    • Peer Reviewed
  • [Journal Article] Comparative Analysis of MicroRNA-mRNA Expression Profiles of Mesenchymal Stem Cells and Dental Follicle Cells2012

    • Author(s)
      Ogura N, Takahashi K, Iwai S, Fujimoto Y, Hashimoto H, Kondoh T
    • Journal Title

      Int J Oral-Med Sci

      Volume: 11 Pages: 13-21

    • URL

      http://www.mascat.nihon-u.ac.jp/labo/info/index.html

    • Peer Reviewed
  • [Presentation] Analysis of microRNA-mRNA expression profiles in dental follicle cells2013

    • Author(s)
      Tomoki R, Ogura N, Takahashi K, Aonuma H, Ishigami D, Ito K, Kamino Y, Kondoh T
    • Organizer
      International Association for Dental Research
    • Place of Presentation
      Seattle, WA, USA
    • Year and Date
      2013-03-23
  • [Presentation] microRNA profiling of dental follicle cells during osteogenic differentiation2013

    • Author(s)
      Takahashi K, Ogura N, Tomoki R, Aonuma H, Ishigami D, Ito K, Kamino Y, Kondoh T
    • Organizer
      International Association for Dental Research
    • Place of Presentation
      Seattle, WA, USA
    • Year and Date
      2013-03-23
  • [Presentation] Osteogenic potential of human dental follicle cells2013

    • Author(s)
      Iwai S, Kuyama K, Kuboyama N, Ogura N, Eda T, Yamamoto H, Kondoh T
    • Organizer
      nternational Association for Dental Research
    • Place of Presentation
      Seattle, WA, USA
    • Year and Date
      2013-03-23
  • [Presentation] ヒト歯嚢由来細胞が骨芽細胞へ分化する過程におけるBMP6の影響2012

    • Author(s)
      高橋康輔,小倉直美,友木里沙,青沼陽菜,藤本陽子,岩井聡,石上大輔,伊藤耕,神野良一,近藤壽郎
    • Place of Presentation
      横浜
    • Year and Date
      2012-10-21
  • [Presentation] ヒト歯嚢由来細胞のLIF発現とLIF標的microRNA発現解析2012

    • Author(s)
      藤本陽子,小倉直美,近藤壽郎,神野良一,伊藤耕,青沼陽菜,高橋康輔,友木里沙
    • Organizer
      日本口腔外科学会
    • Place of Presentation
      横浜
    • Year and Date
      2012-10-20
  • [Presentation] ヒト歯嚢由来細胞の石灰化過程における血管申請因子の検討2012

    • Author(s)
      友木里沙,小倉直美,高橋康輔,青沼陽菜,枝卓志,岩井聡,石上大輔,伊藤耕,神野良一,近藤壽郎
    • Organizer
      日本口腔外科学会
    • Place of Presentation
      横浜
    • Year and Date
      2012-10-19
  • [Presentation] 骨髄由来未分化間葉系幹細胞と歯嚢由来細胞間でのmicroRNA-mRNA発現解析2012

    • Author(s)
      小倉直美,高橋康輔,青沼陽菜,伊藤耕,神野良一,近藤壽郎
    • Organizer
      日本骨代謝学会
    • Place of Presentation
      東京
    • Year and Date
      2012-07-19
  • [Presentation] ヒト歯嚢細胞の石灰化過程での遺伝子発現およびシグナル伝達解析2011

    • Author(s)
      高橋康輔,小倉直美,青沼陽菜,岩井聡,藤本陽子,伊藤耕,神野良一,近藤壽郎
    • Organizer
      日本骨代謝学会
    • Place of Presentation
      大阪
    • Year and Date
      2011-07-28

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Published: 2015-07-16  

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