2013 Fiscal Year Final Research Report
Improvement of outcome of forebrain ischemia by use of dental pulp derived neurospheres
| Project/Area Number |
23592997
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KANAZAWA Kaoru 北海道医療大学, 歯学部病態機能・生理学系 歯科麻酔科学分野, 助教 (40453279)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 前脳虚血モデル / 歯髄 / neurosphere / ラット |
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| Research Abstract |
To improve outcome of global cerebral ischemic pathophysiology, the effect of neurospheres, which was formed from side population cells in dental pulp tissue, were examined. Cerebral expressions of cytokines and BDNF, brain derived neuronal factor, were also examined as expected mechanisms of neuroprotection associated with neurosphere treatment. Post-ischemic intravenous implantation of neurosphere-derived cells after 3 hours of reperfusion improved histological outcome. However, mRNA expressions either of cytokines or BDNF were not significantly different among groups. It was surmised that outcome improvement from neurosphere treatment was caused by mechanisms other than cerebral inflammation process or BDNF overexpression.
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