2013 Fiscal Year Final Research Report
The role of autophagy in cardioprotection by volatile anesthetics
| Project/Area Number |
23593008
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | Osaka Dental University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
DOMAE Naochika 大阪歯科大学, 歯学部, 名誉教授 (60115889)
KANEDA Kazuhiro 大阪歯科大学, 歯学部, 講師(非常勤) (90533886)
SUGIOKA Shingo 大阪歯科大学, 歯学部, 講師 (90278573)
KOTANI Junichiro 大阪歯科大学, 歯学部, 教授 (40109327)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | sevoflurane / heart / cardioprotection / autophagy / ischemia |
|---|
| Research Abstract |
Sevoflurane preconditioning(SEVO-PC) is lost if the ischemic insult is too long. We examined whether induction of autophagy prolongs SEVO-PC protection during a longer ischemic insult.Isolated guinea pigs hearts were subjected to 30 or 45min ischemia,followed by 120min reperfusion. Anesthetic preconditioning was elicited with 2% sevoflurane for ten minutes prior to ischemia. Infarct size sparing effect of SEVO-PC was seen in 30min ischemia, but not 45min ischemia.Induction of autophagy by chloramphenicol prior to ischemia restored the infarct size limiting effect of SEVO-PC lost by 45min ischemia. Western blot analysis showed that expression of Akt and glycogen synthase kinase 3beta was enhanced by induction of autophagy.The effect of autophagy on calcium-induced mitochondrial permeability transition pore(MPTP) opening in isolated mitochondria from cardiomyocytes was assessed.The restoration of SEVO-PC lost by 45min ischemia is associated with enhanced inhibition of MPTP by autophagy.
|
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] セボフルラン・エタノールによる心筋プレコンディショイングの細胞内メカニズムの解明2012
Author(s)
大西杏奈,宮前雅見,金田一弘,大草知佳,稲村吉高,塩見真由美,越沼静,小谷順一郎,高瀬奈緒,古北まゆ子,立石智子,高石和美,江口覚,富岡重正,北畑洋
Organizer
第9回日本歯科麻酔学会中国・四国地方会
Place of Presentation
岡山大学,岡山市
Year and Date
2012-07-29
-
-
-
[Presentation] Cerebral protection effects of lidocaine using a transient cortical ischemia model2012
Author(s)
Momota Y, Shibata H, Nakagomi T, Nakano- Doi A, Okusa C, Miyamae M, Matsuyama T, Kotani J
Organizer
13^<th> International Dental Congress on Anesthesia, Sedation, and Pain Control
Place of Presentation
Cona, Hawaii USA
Year and Date
2012-03-02
-
-
[Presentation] Expression of neural stem cells induced by transient cortical ischemia in mice2011
Author(s)
Momota Y, Nakagomi T, Nakano-Doi A, Saino O, Kasahara Y, Taguchi A, Tanaka M, Tsukamoto Y, Miyamae M, Kotani J, Matsuyama T
Organizer
Abstracts XXV^<th> International Symposium on Cerebral Blood Flow, Metabolism, and Function 2011
Place of Presentation
Barcelona, Spain
Year and Date
20110525-28
-
-
[Presentation] Sevoflurane postconditioning reduces myocardial ischemia-reperfusion injury by induction of autophagy in isolated guinea pig hearts2011
Author(s)
Inamura Y, Miyamae M, Kaneda K, Okusa C, Onishi A, Okamura S, Shiomi M, Momota Y, Figueredo VM, Kotani J
Organizer
9^t h International Congress on Coronary Artery Disease (ICCAD) from Prevention to Intervention 2011
Place of Presentation
Venice, Italy
Year and Date
2011-10-25
-
[Presentation] Dipyridamole-induced cardiac preconditioning persists for 3 days after discontinuing therapy and is mediated through Akt activation2011
Author(s)
Okusa C, Miyamae M, Kaneda K, Inamura Y, Onishi A, Shiomi M, Okamura S, Momota Y, Kotani J, Figueredo VM
Organizer
9^t h International Congress on Coronary Artery Disease (ICCAD) from Prevention to Intervention 2011
Place of Presentation
Venice, Italy
Year and Date
2011-10-24
-
-
-
