2013 Fiscal Year Final Research Report
Basic research for drug discovery: Modulation of early chondrogenesis by the interaction of CCN2 and CCN3
| Project/Area Number |
23593014
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Asahi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KONDOH Nobuo 朝日大学, 歯学部口腔生化学分野, 教授 (40202072)
TAKAYA Eiji 朝日大学, 歯学部口腔生化学分野, 准教授 (70533446)
KAMIYA Masako 朝日大学, 歯学部口腔生化学分野, 助教 (80181907)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 創薬 / 再生医療 / 軟骨 / CCNファミリー / 肢芽 / 高密度培養 / ERK1/2 / MAPK |
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| Research Abstract |
CCN2 is best known as a promoter of chondrocyte proliferation and differentiation among the CCN family members. However, little is known concerning roles of the other CCN member, CCN3 in chondrocytes. These two proteins consists of 4 conserved modules that are highly interactive with a number of biomolecules. In the present study, we demonstrated that CCN3 protein promotes early chondrogenesis based on the following observations: 1) CCN3 was expressed in the embryonic limb mesenchymal cells, whereas, CCN2 was highly expressed in differentiated chondrocytes. 2) Exogenous recombinant CCN3 promoted the proliferation of limb mesenchymal cells in plane culture, and induced proteoglycan accumulation in micromass cultures. 3) By Ccn3 knockdown, chondrogenesis was inhibited in cultures. In addition, we found that extracellular signal-regulated kinase 1/2 pathway was involved in the CCN3-mediated modification of mesenchymal cell and chondroblast activities.
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Research Products
(27 results)
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[Journal Article] CCN2 as a novel molecule supporting energy metabolism of chondrocytes2014
Author(s)
Maeda-Uematsu A, Kubota S, Kawaki H, Kawata K, Miyake Y, Hattori T, Nishida T, Moritani N, Lyons KM, Iida S, Takigawa M
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Journal Title
J Cell Biochem
Volume: 115
Pages: 854-865
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[Journal Article] Compressive Force-Produced CCN2 Induces Osteocyte Apoptosis Through ERK1/2 Pathway2013
Author(s)
Hoshi K, Kawaki H, Takahashi I, Takeshita N, Seiryu M, Murshid SA, Masuda T, Anada T, Kato R, Kitaura H, Suzuki O, Takano-Yamamoto T
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Journal Title
J Bone Miner Res
Volume: (in press)
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[Journal Article] Differential roles of CCN family proteins during osteoblast differentiation : Involvement of Smad and MAPK signaling pathways2011
Author(s)
Kawaki H, Kubota S, Suzuki A, Suzuki M, Kohsaka K, Hoshi K, Fujii T, Lazar N, Ohgawara T, Maeda T, Perbal B, Takano-Yamamoto T, Takigawa M
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Journal Title
Bone
Volume: 49
Pages: 975-989
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[Journal Article] Association of the metastatic phenotype with CCN family members among breast and oral cancer cells2011
Author(s)
Ohgawara T, Kubota S, Kawaki H, Kurio N, Abd El Kader T, Hoshijima M, Janune D, Shimo T, Perbal B, Sasaki A, Takigawa M
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Journal Title
J Cell Commun Signal
Volume: 5
Pages: 291-299
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[Presentation] 圧縮力負荷に伴い骨細胞より産生されたCCN2はERK1/2経路を介しアポトーシスを誘導する2014
Author(s)
星健治,川木晴美,高橋一郎,竹下信郎,清流正弘, Sakhr A Murshid,益田泰輔,穴田貴久,加藤龍史,北浦英樹,鈴木治,山本照子
Organizer
第53回日本生体医工学会大会
Place of Presentation
仙台
Year and Date
20140624-26
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[Presentation] 軟骨細胞のエネルギー代謝を支えるCCN2/CTGF2013
Author(s)
前田彩,久保田聡,三宅由晃,河田かずみ,西田崇,服部高子,森谷徳文,川木晴美, Karen M. Lyons,飯田征二,滝川正春
Organizer
第26回日本軟骨代謝学会
Place of Presentation
豊中
Year and Date
20130301-02
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[Presentation] CCNファミリータンパク質によるMAPK経路を介した骨芽細胞増殖・分化調節2011
Author(s)
川木晴美,久保田聡,鈴木誠,高坂久美子,星健治,加藤龍史,高山英次,神谷真子, Bernard Perbal,山本照子,近藤信夫,滝川正春
Organizer
第4回日本CCNファミリー研究会
Place of Presentation
岡山
Year and Date
2011-08-27
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