2013 Fiscal Year Final Research Report
Optimization of bone regeneration by use of mesenchymal stem cells and its application for closure of jaw cleft
| Project/Area Number |
23593029
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
KOTARO Tanimoto 広島大学, 医歯薬保健学研究院, 教授 (20322240)
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| Co-Investigator(Kenkyū-buntansha) |
TANNE Kazuo 広島大学, 医歯薬保健学研究院, 名誉教授 (30159032)
MASARU Sugiyama 広島大学, 医歯薬保健学研究院, 教授 (70187681)
TANNE Yuki 広島大学, 医歯薬保健学研究院, 助教 (50526241)
KUNIMATSU Ryo 広島大学, 医歯薬保健学研究院, 助教 (40580915)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 歯科矯正学 |
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| Research Abstract |
The purpose of our study is to establish bone regeneration of jaw cleft by use of bone marrow-derived mesenchymal stem cells (MSCs) with a new scaffold, carbonated-hydroxyapatite (CAP). Artificial jaw cleft was created on the both side of maxilla in Beagle dogs. MSCs were derived from bone marrow of iliac bone by use of an aspiration needle, and cultured. After 1 month, MSCs and new scaffold material, CAP granulated powder, were transplanted into the artificial bone defects. Bone regeneration at jaw cleft site was achieved successfully by the transplantation of MSCs/CAP after 3 months. It is also demonstrated suggested that CAP particles could be digested rapidly by osteoclasts. In addition, orthodontic tooth movement into regenerated bone was also achieved. It is emphasized that MSCs/CAP transplantation therapy has a great potential as a new treatment modality for bone regeneration in CLP patients with bone defect due to jaw cleft.
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