Development of a New Treatment in Orthodontics by Focusing on Clock Genes which are Regulated by microRNAs.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23593042
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Iwate Medical University
• Principal Investigator: KAGIYA TADAYOSHI 岩手医科大学, 歯学部, 助教 (30405774)
• Co-Investigator(Renkei-kenkyūsha): NAKAYAMA Takahiro 岩手医科大学, 歯学部, 研究員 (70599208) ; ANDO Yoshinori 岩手医科大学, 歯学部, 助教 (40583662) ; FUJIMURA Akira 岩手医科大学, 歯学部, 教授 (80173459) ; MIURA Hiroyuki 岩手医科大学, 歯学部, 教授 (00048563)
• Project Period (FY): 2011 – 2013
• Keywords: microRNA / Exosomes / Microvesicles / Osteoclasts / Osteoblasts / Macrophages / Microarray / Orthodontics

Research Abstract

The aim of this study is to develop a new treatment in orthodontics by focusing on clock genes which are regulated by microRNAs. Then we investigated osteoclasts, which play an important role in orthodontics. Specifically, we investigated eight miRNAs deemed important for osteoclastogenesis in our study: let-7e, miR-21, miR-33, miR-155, miR-210, miR-223, miR-378, and miR-1224. The results suggest that osteoclasts secrete extracellular microvesicles containing specific miRNAs, but that they do not contain the entire set of intracellular miRNAs.

Research Products

• [Journal Article] Expression of MicroRNAs in the Extracellular Microvesicles of Murine Osteoclasts (2013)
  • Author(s): Kagiya T, Taira M
  • Journal Title: J Oral Tissue Engin Volume: 10(3) Pages: 142-150
  • URL: https://iwatemed.repo.nii.ac.jp/
  • Peer Reviewed
• [Journal Article] Expression profiling of microRNAs in RAW264.7 cells treated with a combination of tumor necrosis factor alpha and RANKL during osteoclast differentiation (2013)
  • Author(s): Kagiya T, Nakamura S
  • Journal Title: J Periodontal Res Volume: 48(3) Pages: 373-385
  • DOI: 10.1111/jre.12017
  • Peer Reviewed
• [Presentation] microRNAを介した破骨細胞分化制御について (2014)
  • Author(s): 鍵谷忠慶
  • Organizer: 岩手医科大学歯学研究セミナー
  • Place of Presentation: 盛岡市
  • Year and Date: 2014-03-04
• [Presentation] 破骨細胞分化において重要と考えられるmicroRNAについて (2013)
  • Author(s): 鍵谷忠慶、中山貴博、安藤禎紀、藤村朗
  • Organizer: 第118回日本解剖学会全国学術集会
  • Place of Presentation: 高松市
  • Year and Date: 2013-03-28
• [Presentation] NF-α/RANKL誘導性破骨細胞におけるmicroRNAの発現について (2012)
  • Author(s): 鍵谷忠慶、中山貴博
  • Organizer: 第117回日本解剖学会全国学術集会
  • Place of Presentation: 甲府市
  • Year and Date: 2012-03-26
• [Presentation] 赤色蛍光強発現TGマウスの蛍光分布 (2012)
  • Author(s): 安藤禎紀、古川真司、桑島幸紀、鍵谷忠慶、藤村朗、三浦廣行
  • Organizer: 第117回日本解剖学会全国学術集会
  • Place of Presentation: 甲府市
  • Year and Date: 2012-03-26
• [Presentation] 骨代謝におけるmicroRNAの役割について (2012)
  • Author(s): 鍵谷忠慶
  • Organizer: 東夷塾
  • Place of Presentation: 甲府市
  • Year and Date: 2012-03-26
• [Presentation] マクロファージが銅イオンに暴露された時のmicroRNAの発現について (2011)
  • Author(s): 鍵谷忠慶、平雅之、安藤禎紀、藤村朗
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜市
  • Year and Date: 2011-10-02
• [Book] Nova Science Publishers (2014)
  • Author(s): Kagiya T, Taira M
  • Total Pages: 69-80
  • Publisher: A New Application for Microarrays : Analysis of Global MicroRNA Expression Profiles in the Extracellular Microvesicles of Human Macrophage-like Cells.In : Rogers JV, editor. Microarrays : Principles, Applications and Technologies
• [Remarks] 岩手医科大学ホームページ
  • URL: http://www.iwate-med.ac.jp/
• [Remarks] 岩手医科大学リポジトリ
  • URL: https://iwatemed.repo.nii.ac.jp/