2013 Fiscal Year Final Research Report
Development of periodontitis-preventive methods using epigenetically controling food ingredient
| Project/Area Number |
23593104
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Social dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | エピジェネティクス / 骨吸収 / 歯周炎 / 予防法 |
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| Outline of Final Research Achievements |
To establish preventive methods against periodontitis-derived bone resorption, we first examine the effects of BIX01294 and chaetocin, which are inhibitors of H3K9 methyltransferase, on RANKL-induced osteoclast differentiation. Both inhibitors dose-dependently inhibited RANKL-induced osteoclast differentiation of preosteoclast-like Raw264.7 cells. BIX01294 and chaetocin rescues RANKL-induced downregulation of osteoclast-differentiation-inhibitory gene expressions in a dose-dependent manner. However, siRNA for G9a did not inhibit RANKL-induced osteoclast differentiation, suggesting that the effects of BIX01294 on osteoclast differentiation are not derived from G9a inhibition. In addition, chaetocin dose dependently inhibited RANKL-induced downregulation of blimp1 expression, which is reportedly inhibits osteoclast differentiation inhibitory genes.
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| Free Research Field |
医歯薬学
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