2013 Fiscal Year Final Research Report
Mechanism of Micromolar NaF in anti-osteoclastogenesis
| Project/Area Number |
23593110
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Social dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
ARAKAWA Hirohisa 神奈川歯科大学, 歯学研究科(研究院), 教授 (00130906)
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| Co-Investigator(Kenkyū-buntansha) |
BHAWAL Ujjal 日本大学, 松戸歯学部, 助教 (50433339)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 低濃度フッ化物 / 骨芽細胞 / 破骨細胞 |
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| Research Abstract |
We report here that Sprague-Dawley rat tissue samples of P. gingivalis ATCC 33277 group with bone resorption visible on micro computed tomography, exhibited a marked increase in cathepsin K, NFATc1, MMP9, and IL-1beta expression compared with those of P. gingivalis + NaF group. Specimens were also confirmed by staining with tartrate-resistant acid phosphatase and by measuring the distance between the cemento-enamel junction and the alveolar bone crest. Moreover, bone marrow cells formed osteoclasts in vitro with exogenous receptor activator of RANKL or M-CSF and micromolar NaF inhibited osteoclast formation. Additionally, cultured bone marrow cells of NaF group expressed higher levels of osteoprotegerin than did control group. These results suggest a model for understanding the pathogenesis of aggressive bone loss in periodontitis and anti-osteoclastogenesis function of micromolar NaF.
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Research Products
(39 results)
