2013 Fiscal Year Final Research Report
Effects of food ingredients to cariogenic biofilms and alpha-glucans.
| Project/Area Number |
23593118
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Social dentistry
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| Research Institution | Iwate Biotechnology Research Center |
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Principal Investigator |
YANO Akira 公益財団法人岩手生物工学研究センター, 生物資源研究部, 主席研究員 (50312286)
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| Project Period (FY) |
2011 – 2013
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| Keywords | バイオフィルム / αグルカン / デキストラン |
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| Research Abstract |
The alpha-glucans are thought to be an anchor molecule for formation of oral biofilms. We confirmed this hypothesis by in vitro culture using the glucan fixed culture plate. The alpha-1,3-glucan, alpha-1,6-glucan and alpha-1,3-1,6-glucan are independently prepared and fixed on the wells of 96-well plates. Streptococcus mutans was cultured on this plate by the sucrose free medium, and the biofilm formation was observed after O/N incubation. Interestingly, initial attachment of S. mutans on the glucan plate was weaker than on the saliva coating plate. The other oral Streptococci were also tested but biofilm formation were not observed as like as S. mutans. The function of GTFs to biofilm formation were investigated by E. coli transformants expressing GTFs. Those E. coli could form biofilms on the glucan fixed palte.
During the research, we found that soluble alpha-glucan, dextran, could be used as biofilm decolonizing substrate.
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