2013 Fiscal Year Final Research Report
Elucidation of protein secreting mechanisms of filamentous fungi by the comparative genomic analysis
| Project/Area Number |
23603002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomass energy
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| Research Institution | Nagaoka University of Technology |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 糸状菌 / 比較ゲノム解析 / セルロース / セルラーゼ |
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| Research Abstract |
The cellulolytic filamentousfungus Trichoderma reesei secrets a tremendous amount of proteins and almost all of these proteins are cellulases and hemicellulases. Therefore, T. reesei is an ideal model organism to understand protein secretion mechanism of filamentous fungi. The goal of this study is to understand cellulase production mechanisms of T. reesei through comparative genomic analysis of a cellulase hyper-producing mutant lineage developed in Japan. After sequencing the genome of T. reesei mutants, by a Next-Generation Sequencer, a number of single nucleotide polymorphisms (SNPs) were identified. We focused on three mutated genes which are cre1 encoding the carbon catabolite repressor, bglr encoding transcriptional activator of intracellular beta-glucosidase, and bgl2 encoding major intracellular beta-glucosidase BGLII. From gene disruption and complementation of mutated nucleotide, these SNPs were found to be responsible for increased cellulase production.
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[Remarks] 長岡技術科学大学博士論文「Trichoderma reeseiの形態・比較ゲノム解 析に基づくセルラーゼ高生産化機構の解明」 新田美貴子, 2012年6月
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[Remarks] 長岡技術科学大学博士論文「Molecular Evolution of Trichoderma reesei Hypercellulolytic Mutants(Trichoderma reesei) Porciuncula Juliano, 2014年3月
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[Remarks] 長岡技術科学大学修士論文「比較ゲノム解析によるTrichoderma reesei日本型系統樹進化の謎に迫る〜bgl2のSNPが セルラーゼ高生産化に与える影響〜」 山口香織, 2013年3月
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