2013 Fiscal Year Final Research Report
Total photon flux measurement of dim LED reference light source
| Project/Area Number |
23615013
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Reliable environmental measurement methods
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
KAZUKI Niwa 独立行政法人産業技術総合研究所, 計測標準研究部門, 主任研究員 (30443211)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Dai-ichiro 兵庫県立大学, 大学院工学研究科 (60423901)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAYA Takanori 浜松ホトニクス株式会社, 電子管事業部 (60600690)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 光放射測定 / 分光計測 / LED / 積分球 / 全放射束 / 生物発光 / 量子収率 / 微弱光 |
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| Research Abstract |
For the purpose of quantitative photon measurements in bioluminescence assays, we measured photon number emitted from reference light sources. For precise and reliable absolute measurements, we evaluated uncertainty of our integrating sphere spectral radiometer in accordance with the methodologies for spectral radiometry standards. This integrating sphere system had been specially designed and constructed to measure quite low level intensity of photons from bioluminescence reaction and calibrated using a national standard traceable spectral irradiance standard lamp. We also investigate quantum yield and other enzyme parameters of mutant luciferases to contribute the understanding the biochemistry of firefly bioluminescence reaction. These quantitative basis of photon measurement will contribute the applications of bioluminescence reaction, which is increasingly employed as gene expression marker to monitor status of cellular activity.
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