2013 Fiscal Year Final Research Report
Role of histone H3K4 demethylase KDM1B for oocyte-specific imprinting in mice
| Project/Area Number |
23616006
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
epigenetics
|
|---|
| Research Institution | Tokyo University of Agriculture |
|---|
Principal Investigator |
OBATA Yayoi 東京農業大学, 応用生物科学部, 准教授 (70312907)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | ゲノム刷込み / 生殖細胞 |
|---|
| Research Abstract |
To understand the role of histone H3K4 demethylase KDM1B for oocyte-specific imprinting, expression of KDM1B during oocyte growth was analyzed and the establishment of methylation imprints in Zac1 and Mest loci were examined under conditions of DNA methyltransferases (DNMTs) overexpression. The results showed that KDM1B mRNA was expressed at a high level in oocytes from the non-growing stage but KDM1B protein was expressed from the growth stage onward. Excess of DNMTs induced early acquisition of DNA methylation imprints at Zac1 but not Mest in early-growing oocytes. Thus, we revealed that the presence of DNMTs and KDM1B is essential but not sufficient for establishment of oocyte-specific imprinting.
|
-
-
-
-
-
[Journal Article] Contribution of intragenic DNA methylation in mouse gametic DNA methylomes to establish oocyte-specific heritable marks2012
Author(s)
Kobayashi H, Sakurai T, Imai M, Takahashi N, Fukuda A, Obata Y, Sato S, Nakabayashi K, Hata K, Sotomaru Y, Suzuki Y, Kono T
-
Journal Title
PLoS Genet
Volume: 8
Pages: e1002440
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
