Introduction of Caenorhabditis elegans to evaluate and elucidate the anti-senescence effects of food factors and derivatives

2013 Fiscal Year Final Research Report

• Project/Area Number: 23617017
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrated Nutrition Science
• Research Institution: Osaka City University
• Principal Investigator: NISHIKAWA Yoshikazu 大阪市立大学, その他の研究科, 教授 (60183539)
• Co-Investigator(Kenkyū-buntansha): KONISHI Yotaro 大阪市立大学, 大学院生活科学研究科, 教授 (70116812) ; INUI Hiroshi 大阪市立大学, 生命環境科学研究科, 教授 (20193568) ; KIKUZAKI Hiroe 奈良女子大学, 生活環境学部, 教授 (60291598) ; TAKADA Jiro 福岡大学, 薬学部, 教授 (90122704)
• Project Period (FY): 2011 – 2013
• Keywords: 線虫 / 老化 / 生体防御 / 栄養 / 抗酸化物質 / 乳酸菌 / 自然免疫 / 機能性食品因子

Research Abstract

The nematode, Caenorhabditis elegans, was used to screen lipid-soluble substances, particularly antioxidants, for the lifespan-prolonging effect. Tocotrienols, astaxanthin, sesamin, and the cell wall of bifidobacteria could extend the lifespan of the nematode. These substances enhanced the worm's tolerance to stresses due to heat, UV irradiation, oxidative chemicals, or bacterial infection; these substances could protect the worms from aging-associated weakness of locomotive ability. Each substance gave the different phenotype of tolerance. The mutants which have loss-of-function genes on the signal induction pathways of p38 MAPK or Insulin/IGF for host defense were used to elucidate the mechanism of the anti-senescence effects. Each functional food factor was definitely dependent on these pathways in different manners.

Research Products

• [Journal Article] Influence of oral supplementation with sesamin on longevity of Caenorhabditis elegans and the host defense (2014)
  • Author(s): Yaguchi, Y., Komura, T., Kashima, N., Tamura, M., Kage-Nakadai, E., Saeki, S., Terao, K., Nishikawa, Y.
  • Journal Title: Eur. J. Nutr
  • DOI: 10.1007/s00394-014-0671-6
  • Peer Reviewed
• [Journal Article] Effects of Popping on Nutrient Contents of Amaranth Seed, Plant Foods Hum (2014)
  • Author(s): Murakami, T., Yutani, A., Yamano, A., Iyota, H., Konishi, Y.
  • Journal Title: Nutr Volume: 69 Pages: 25-29
  • Peer Reviewed
• [Journal Article] Mechanism underlying prolongevity induced by bifidobacteria in Caenorhabditis elegans (2013)
  • Author(s): Komura, T., Ikeda, T., Yasui, C., Saeki, S., Nishikawa, Y.
  • Journal Title: Biogerontology Volume: 14 Pages: 73-87
  • DOI: 10.1007_s10522-012-9411-6
  • Peer Reviewed
• [Journal Article] Identification of carbonyl reductase 1 as a resveratorol-binding protein by affinity chromatography using 4'-amino-3,5-dihydroxy-trans-stilbene (2013)
  • Author(s): Ito, Y., Mitani, T., Harada, N., Isayama, A., Tanimori, S., Takenaka, S., Nakano, Y., Inui, H., Yamaji, R.
  • Journal Title: J. Nutr. Sci. Vitaminol Volume: 59 Pages: 358-364
  • Peer Reviewed
• [Journal Article] Oligosaccharides from agar inhibit murine intestinal inflammation through the induction of heme oxygenase-1 expression (2013)
  • Author(s): Higashimura, Y., Naito, Y., Takagi, T., Mizushima, K., Hirai, Y., Harusato, A., Ohnogi, H., Yamaji, R., Inui, H., Nakano, Y., Yoshikawa, T.
  • Journal Title: J. Gastroenterol Volume: 48 Pages: 897-909
  • Peer Reviewed
• [Journal Article] The preventive effect of b-carotene on denervation-induced soleus muscle atrophy in mice (2013)
  • Author(s): Ogawa, M., Kariya, Y., KItakaze, T., Yamaji, R., Harada, N., Sakamoto, T., Hosotani, K., Nakano, Y., Inui, H.
  • Journal Title: Br. J. Nutr Volume: 109 Pages: 1349-1358
  • Peer Reviewed
• [Journal Article] Inhibitory activity of Filipendula ulmaria constituents on recombinant human histidine decarboxylase (2013)
  • Author(s): Nitta, Y., Kikuzaki, H., Azuma, T., Ye, Y., Sakaue, M., Higuchi, Y., Komori, H., Ueno, H.
  • Journal Title: Food Chemitsry Volume: 138 Pages: 1551-1556
  • Peer Reviewed
• [Journal Article] Increase in secretory sphingomyelinase activity and specific ceramides in the aorta of apolipoprotein E knockout mice during aging (2013)
  • Author(s): Kobayashi, K., Nagata, E., Sasaki, K., Harada-Shiba, M., Kojo, S., Kikuzaki, H.
  • Journal Title: Biol. Pharm. Bull Volume: 36 Pages: 1192-1196
  • Peer Reviewed
• [Journal Article] Interrelationships among seed yield, total protein and amino acid composition of ten quinoa (Chenopodium quinoa) cultivars from two different agroecological regions (2012)
  • Author(s): Juan A Gonzalez, J. A., Konishi, Y., Bruno, M., Valoy, M., Prado, F. E.
  • Journal Title: J. Sci.Food Agric Volume: 92 Pages: 1222-1229
  • Peer Reviewed
• [Journal Article] Development of a method for oral administration of hydrophobic substances to Caenorhabditis elegans : pro-longevity effects of oral supplementation with lipid-soluble antioxidants (2012)
  • Author(s): Kashima, N., Fujikura, Y., Komura, T., Fujiwara, S., Sakamoto, M., Terao, K., Nishikawa, Y.
  • Journal Title: Biogerontology Volume: 13 Pages: 337-344
  • DOI: 10.1007/s10522-012-9378-3
  • Peer Reviewed
• [Journal Article] Identification of a novel glycoside, leptosin, as a chemical marker of manuka honey (2012)
  • Author(s): Kato, Y., Kikuzaki, H.(他4名)
  • Journal Title: J. Agric. Food Chem Volume: 60 Pages: 418-3423
  • Peer Reviewed
• [Journal Article] Antimutagenic andα-glucosidase inhibitory effects of constituents from Kaempferia parviflora (2011)
  • Author(s): Azuma, T., Kikuzaki, H. (他4名)
  • Journal Title: Food Chemistry Volume: 125 Pages: 471-475
  • Peer Reviewed
• [Presentation] Rosa gallicaの花弁に含まれるヒスチジン脱炭酸酵素阻害活性成分の探索 (2014)
  • Author(s): 菊﨑泰枝(他5名)
  • Organizer: 日本農芸化学会2014年大会
  • Place of Presentation: 明治大学
  • Year and Date: 2014-03-30
• [Presentation] 線虫(Caenorhabditis elegans)におけるセサミンの寿命延長機構に関する研究 (2013)
  • Author(s): 矢口由紀恵、小村智美、加嶋倫子、田村美帆、寺尾啓二、西川禎一
  • Organizer: 日本栄養・食糧学会第52回近畿支部大会
  • Place of Presentation: 滋賀県立大学(p.34)
  • Year and Date: 2013-10-26
• [Presentation] 線虫(Caenorhabditis elegans)におけるアスタキサンチンの寿命延長効果 (2013)
  • Author(s): 藤原翔吾、鍋島明日香、寺尾啓二、西川禎一
  • Organizer: 日本栄養・食糧学会第52回近畿支部大会
  • Place of Presentation: 滋賀県立大学(p.41)
  • Year and Date: 2013-10-26
• [Presentation] 1,5-アンヒドログルシトールの物理化学的性質と利用法 (2013)
  • Author(s): 三宅千悠、太田沙織、小西洋太郎
  • Organizer: 日本栄養・食糧学会第52回近畿支部大会
  • Place of Presentation: 滋賀県立大学(講演要旨集p.25)
  • Year and Date: 2013-10-26
• [Presentation] 発芽玄米を使用した高品質・機能性パン作りに関する研究 (2013)
  • Author(s): 志方万莉奈、岡田真実、佐橋磨衣子、村田絵美、横山実香、小西洋太郎
  • Organizer: 日本栄養・食糧学会第52回近畿支部大会
  • Place of Presentation: 滋賀県立大学(講演要旨集p.25)
  • Year and Date: 2013-10-26
• [Presentation] 糖負荷ラットの血糖値に及ぼす1,5-アンヒドログルシトールによる影響 (2013)
  • Author(s): 中村友美、棚橋梨江、大橋純子、小西洋太郎
  • Organizer: 日本応用糖質科学会平成25年度大会
  • Place of Presentation: 鹿児島大学(講演要旨集p.50)
  • Year and Date: 2013-09-25
• [Presentation] 1,5-アンヒドログルシトールの甘味評価 (2013)
  • Author(s): 隅蔵菜海、太田沙織、向瀬きなみ、金一玲、小西洋太郎
  • Organizer: 日本応用糖質科学会平成25年度大会
  • Place of Presentation: 鹿児島大学(講演要旨集p.50)
  • Year and Date: 2013-09-25
• [Presentation] Influence of Bifidobacterium infantis on lifespan and aging-related biomarkers of Caenorhabditis elegans (2012)
  • Author(s): Komura, T., and Nishikawa, Y.
  • Organizer: The 2nd International Conference on Model Hosts
  • Place of Presentation: Rhodes, Greece, Abstract(35)
  • Year and Date: 20120901-06
• [Presentation] Development of a method for oral administration of hydrophobic substances to Caenorhabditis elegans; prolongevity effects of oral supplementation with sesamin (2012)
  • Author(s): Yaguchi, Y., Kashima, N., Komura, T., Terao, K., and Nishikawa, Y.
  • Organizer: The 2nd International Conference on Model Hosts
  • Place of Presentation: Rhodes, Greece, Abstract(30)
  • Year and Date: 20120901-06
• [Presentation] 線虫(Caenorhabditis elegans)の各種ストレス耐性に及ぼすトコトリエノールの影響 (2012)
  • Author(s): 加嶋倫子、田村美帆、小村智美、藤倉由記子、寺尾啓二、西川禎一
  • Organizer: 第66回日本栄養・食糧学会大会
  • Place of Presentation: 仙台(p.197)
  • Year and Date: 20120518-20
• [Presentation] 線虫(Caenorhabditis elegans)の寿命と各種ストレス耐性に及ぼすセサミンの影響 (2012)
  • Author(s): 矢口由紀恵、小村智美、加嶋倫子、寺尾啓二、西川禎一
  • Organizer: 第66回日本栄養・食糧学会大会
  • Place of Presentation: 仙台(p.197)
  • Year and Date: 20120518-20
• [Presentation] ショウガ科植物Kaempferia parviflora根茎に含まれる熱に安定な色素について (2012)
  • Author(s): 菊﨑泰枝(他5名)
  • Organizer: 日本調理科学会平成24年度大会
  • Place of Presentation: 秋田大学
  • Year and Date: 2012-08-25
• [Presentation] Prolongevity effects of tocotrienols in Caenorhabditis elegans (2012)
  • Author(s): Nishikawa, Y.
  • Organizer: Tocotrienol Symposium in the 103rd Annual American Oil Chemists'Society (AOCS) Meeting
  • Place of Presentation: Long Beach, California, Abstract
  • Year and Date: 2012-04-29
  • Invited
• [Presentation] マヌカ蜂蜜に由来する新規配糖体leptosinのケミカルマーカーとしての有用性について (2012)
  • Author(s): 菊﨑泰枝(他5名
  • Organizer: 日本農芸化学会2012年大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2012-03-24
• [Presentation] Prolongevity effects of tocotrienols in Caenorhabditis elegans (2011)
  • Author(s): Kashima, N., Fujikura, Y., Komura, T., Nishikawa, Y., Terao, K., and Tan, B.
  • Organizer: Food and Function 2011
  • Place of Presentation: Kosice, Slovakia, Abstract(33)
  • Year and Date: 20111025-27
• [Presentation] ビフィズス菌給餌がCaenorhabditis elegans(線虫)のバイオマーカーに与える影響 (2011)
  • Author(s): 小村智美、西川禎一
  • Organizer: 日本食品免疫学会第7回学術大会
  • Place of Presentation: 東京(p.34)
  • Year and Date: 20111018-19
• [Presentation] 線虫(Caenorhabditis elegans)の各種ストレス耐性に及ぼすトコトリエノールの影響 (2011)
  • Author(s): 加嶋倫子、小村智美、西川禎一
  • Organizer: 日本食品免疫学会第7回学術大会
  • Place of Presentation: 東京(p.51)
  • Year and Date: 20111018-19
• [Presentation] 脂溶性抗酸化物質の経口投与がCaenorhabditis elegans(線虫)の寿命に与える影響 (2011)
  • Author(s): 加嶋倫子、藤倉由記子、寺尾啓二、西川禎一
  • Organizer: 第65回日本栄養・食糧学会大会
  • Place of Presentation: 東京(p.146)
  • Year and Date: 20110513-15
• [Presentation] ビフィズス菌給餌によるCaenorhabditis elegansの寿命延長とそのバイオマーカーの検討 (2011)
  • Author(s): 小村智美、西川禎一
  • Organizer: 第65回日本栄養・食糧学会大会
  • Place of Presentation: 東京(p.237)
  • Year and Date: 20110513-15
• [Book] Recent Advances on Model Hosts, Caenorhabditis elegans as an Alternative Model to Study Senescence of Host Defense and the Prevention by Immunonutrition (2012)
  • Author(s): Komura, T., Ikeda, T., Hoshino, K., Shibamura, A., and Nishikawa, Y., (E. Mylonakis,F. M. Ausubel,A. Casadevall,M. Gilmore, (ed.))
  • Total Pages: 19-27
  • Publisher: Springer Science
• [Book] Tocotrienols : Vitamin E Beyond Tocopherols, Second Edition, Prolongevity Effects of Tocotrienols : Trials in Caenorhabditis elegans (2012)
  • Author(s): Kashima, N., Fujikura, Y., Komura, T., Nishikawa, Y., Terao, K., and Tan, B., (B. Tan, R. R. Watson, V. R. Preedy, (ed.))
  • Total Pages: 279-289
  • Publisher: CRC Press
• [Remarks]
  • URL: http://www.life.osaka-cu.ac.jp/cgi/pro.cgi?4102
• [Remarks]
  • URL: http://nishikawa-lab.net/