• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2013 Fiscal Year Final Research Report

Clinical research on the central nervous system disorder by quantitative determination of serum tryptophan metabolites

Research Project

  • PDF
Project/Area Number 23617027
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Integrated Nutrition Science
Research InstitutionToho University

Principal Investigator

IIZUKA Hideaki  東邦大学, 薬学部, 助教 (10057771)

Co-Investigator(Kenkyū-buntansha) FUKUSHIMA Takeshi  東邦大学, 薬学部, 教授 (00272485)
ICHIBA Hideaki  東邦大学, 薬学部, 講師 (80120236)
SADAMOTO Kiyomi  東邦大学, 薬学部, 教授 (00297673)
Project Period (FY) 2011 – 2013
Keywords中枢性精神疾患 / 統合失調症 / Lートリプトファン / L-キヌレニン / セロトニン / LC-MS / HPLC / 蛍光検出
Research Abstract

In the brain tissue (nucleus accumbens and hippocampus) of phencyclidine-treated mice which is an animal model of schizophrenia, the L-tryptophan (L-Trp)concentration was significantly decreased. This result suggested that dysregulation of L-Trp metabolism occurred in these brain regions.
In the present study, a significant gender difference was observed, namely, L-Trp concentration in the sera of male healthy subjects was higher than that of female healthy subjects. A significant correlation was observed between L-Trp and L-KYN concentrations in male, but not in female subjects. The serum levels of L-Trp, KYN, and serotonin were significantly different between controls and schizophrenia patients, suggesting that determinations of L-Trp, KYN, and 5-HT in serum might be crucial for clinical diagnosis.

  • Research Products

    (20 results)

All 2014 2013 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (16 results) Remarks (1 results)

  • [Journal Article] HPLC-fluorescence determination of thiol compounds in the serum of human male and female subjects using HILIC-mode column2014

    • Author(s)
      Kono Y, Iizuka H, Isokawa M, Tsunoda M, Ichiba H, Sadamoto K, Fukushima T
    • Journal Title

      Biomedical Chromatography

      Volume: 28 Pages: 589-593

    • DOI

      10.1002/bmc.3086

    • Peer Reviewed
  • [Journal Article] Decreased L-tryptophan concentration in distinctive brain regions of mice treated repeatedly with phencyclidine2013

    • Author(s)
      Iizuka H, Watanabe S, Koshikawa M, Matsumoto Y, Aoyama Y, Ichiba H, Nabeshima T, Fukushima T
    • Journal Title

      Analytical Bioanalytical Chemistry

      Volume: 405 Pages: 8137-8141

    • DOI

      10.1007/s00216-013-7010-2

    • Peer Reviewed
  • [Journal Article] Determination of L-tryptophan and L-kynurenine in human serum by using LC-MS after derivatization with (R)-DBD-PyNCS2013

    • Author(s)
      Ohashi H, Iizuka H, Yoshihara S, Otani H, Kume M, Sadamoto K, Ichiba H, Fukushima T
    • Journal Title

      International Journal of Tryptophan Research

      Volume: 6 Pages: 9-14

    • DOI

      10.4137/IJTR.S11459

    • Peer Reviewed
  • [Presentation] HPLC-蛍光検出法による統合失調症患者血清中チオール化合物の定量2014

    • Author(s)
      河野由美子
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本市民体育館(熊本県)
    • Year and Date
      2014-03-30
  • [Presentation] HPLC-蛍光検出法による統合失調症患者血清中D-乳酸及び3-ヒドロキシ酪酸の定量2014

    • Author(s)
      大野千尋
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本市民体育館(熊本県)
    • Year and Date
      2014-03-30
  • [Presentation] HPLC-蛍光検出法によるフェンシクリジン慢性投与マウス脳ホモジネート中N-アセチルアスパラギン酸の定量2014

    • Author(s)
      敏蔭望美
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本市民体育館(熊本県)
    • Year and Date
      2014-03-30
  • [Presentation] HPLC-蛍光検出法によるヒト血清中遊離脂肪酸の定量2013

    • Author(s)
      錦織南美
    • Organizer
      第24回クロマトグラフィー科学会議
    • Place of Presentation
      東京大学本郷キャンパス(東京都)
    • Year and Date
      2013-11-12
  • [Presentation] HPLCによる統合失調症患者の血清中アミノ酸定量2013

    • Author(s)
      飯塚英昭
    • Organizer
      第23回日本臨床精神神経薬理学会・第43回日本神経精神薬理学会合同年会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県)
    • Year and Date
      2013-10-25
  • [Presentation] (R)-DBD-PyNCSによる誘導体化およびLC-MSを用いるヒト血清中L-TrpおよびL-キヌレニンの定量2013

    • Author(s)
      飯塚英昭
    • Organizer
      日本Trp研究会第35回学術集会
    • Place of Presentation
      京都大学吉田キャンパス(京都府)
    • Year and Date
      2013-09-29
  • [Presentation] HPLC-蛍光検出法によるヒト血清中グルタチオンおよびシステインの定量2013

    • Author(s)
      河野由美子
    • Organizer
      第26回バイオメディカル分析科学シンポジウム(BMAS 2013)
    • Place of Presentation
      昭和大学旗の台キャンパス(神奈川県)
    • Year and Date
      2013-08-02
  • [Presentation] HPLC-蛍光検出法による生体試料中N-アセチルアスパラギン酸の定量2013

    • Author(s)
      葛丹
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2013-03-28
  • [Presentation] ケタミン連続投与ラット血漿中トリプトファン及びその代謝物濃度の変動解析2013

    • Author(s)
      福島健
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2013-03-28
  • [Presentation] Determination of tryptophan and kynurenic acid concentrations in rats after intraperitoneal administration of tryptophan enantiomers2012

    • Author(s)
      Takeshi Fukushima
    • Organizer
      13th International Society for Tryptophan Research (ISTRY 2012)
    • Place of Presentation
      Sydney(Australia)
    • Year and Date
      2012-11-09
  • [Presentation] Increased kynurenic acid concentration in rat plasma by repeated ketamine administration2012

    • Author(s)
      Takeshi Fukushima
    • Organizer
      13th International Society for Tryptophan Research (ISTRY 2012)
    • Place of Presentation
      Sydney(Australia)
    • Year and Date
      2012-11-09
  • [Presentation] Fluorimetric determination of L-tryptophan in serum or brain homogenate of Sprague-Dawley rats by HPLC using precolumn fluorescence derivatization with R(-)-DBD-PyNCS2012

    • Author(s)
      Hideaki Iizuka
    • Organizer
      13th International Society for Tryptophan Research (ISTRY 2012)
    • Place of Presentation
      Sydney(Australia)
    • Year and Date
      2012-11-08
  • [Presentation] Determination of N-acetylaspartic acid in serum sample by HPLC with fluorescence detection2012

    • Author(s)
      Dan Ge
    • Organizer
      新アミノ酸分析研究会第2様式C-19、F-19、Z-19(共通)回学術講演会
    • Place of Presentation
      東京大学本郷キャンパス(東京都)
    • Year and Date
      2012-10-26
  • [Presentation] ラット線条体マイクロダイアリシスによるD-およびL-トリプトファン投与時における脳内トリプトファンおよびドパミン濃度の変動解析2012

    • Author(s)
      大谷隼斗
    • Organizer
      第56回日本薬学会関東支部大会
    • Place of Presentation
      昭和大学旗の台キャンパス(神奈川県)
    • Year and Date
      2012-10-13
  • [Presentation] (R)-(-)-DBD-PyNCSを用いるプレカラム蛍光誘導体化法によるマウス脳内トリプトファンのHPLC-蛍光定量2011

    • Author(s)
      渡邉祥子
    • Organizer
      第33回日本トリプトファン研究会学術集会
    • Place of Presentation
      東邦大学習志野キャンパス(千葉県)
    • Year and Date
      2011-12-04
  • [Presentation] R(-)-DBD-PyNCSを用いるプレカラム蛍光誘導体化によるトリプトファン光学異性体腹腔内投与におけるラット血漿中のD-およびL-TrpのHPLCによる蛍光定量2011

    • Author(s)
      飯塚英昭
    • Organizer
      第55回日本薬学会関東支部大会
    • Place of Presentation
      東邦大学習志野キャンパス(千葉県)
    • Year and Date
      2011-10-08
  • [Remarks]

    • URL

      http://www.lab.toho-u.ac.jp/phar/Analche/index.html

URL: 

Published: 2015-07-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi