2013 Fiscal Year Final Research Report
A potential modulation of mast cell function by nutritional factors
| Project/Area Number |
23617028
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrated Nutrition Science
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| Research Institution | Sagami Women's University |
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Principal Investigator |
MASUKO Kayo 相模女子大学, 栄養科学部, 教授 (80288208)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 肥満細胞 / 栄養 / ヒスタミン / グルタミン / アルギニン |
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| Research Abstract |
[Background] Mast cell activation has been implicated in wound healing via releasing a variety of mediators. Glutamine (Gln) and arginine (Arg) are used as "immunonutrients" in malnourished patients, but their potential effect on mast cells is unclear. We evaluated the effect of Gln and/or Arg on mast cell function. [Methods] P815 cells were cultured in a medium under different Gln and/or Arg concentrations. Histamine and IL-13 release was measured using enzyme-linked immunoassays. [Results and Discussion] Cells cultured in higher Gln concentrations produced a greater histamine response upon IL-6 stimulation. IL-13 production was not significantly altered by IL-6 stimulation or by Arg concentration. [Conclusions] Gln and/or Arg concentrations in situ may regulate histamine and IL-13 release from mast cells. The appropriate use of functional amino acids as immunonutrients may suppress inflammation and aid in wound healing.
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