2013 Fiscal Year Final Research Report
Basic analysis for evaluating the possibility of phytochemicals as a therapeutic and prophylactic agent for mood disorders
| Project/Area Number |
23617036
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrated Nutrition Science
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SAKAMAKI Ruka 西南女学院大学, 保健福祉学部, 准教授 (80389486)
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| Project Period (FY) |
2011 – 2013
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| Keywords | フィトケミカル / ポリフェノール / 気分障害 / カテコールアミン / ノルアドレナリントランスポーター / セロトニントランスポーター / チロシン水酸化酵素 |
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| Research Abstract |
Resveratrol has attracted wide attention because of its antioxidant, anti-apoptotic and anti-inflammatory effects. We have previously reported that resveratrol inhibits acetylcholine-induced catecholamine secretion and synthesis through suppressing ion influx in cultured bovine adrenal medullary cells. In this study, we examined the effect of resveratrol on noradrenaline (NA) transporter, which is also known to be the cellular targets of antidepressants. Resveratrol stimulated NA uptake by SK-N-SH cells at low concentration, it was inhibited NA uptake at high concentration against this. Kinetics analysis of the effect of resveratrol on NA transport in SK-N-SH cells indicated that they decreased the Vmax of NA transport with little or no change in Km. Scatchard analysis of nisoxetine binding to plasma membranes showed that resveratrol did not affect the Bmax and the Kd.
The present findings suggest that resveratrol plays a biphasic role in the regulation of neuronal NA transport.
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Research Products
(20 results)
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[Journal Article] Effects of food deprivation on the hypothalamic feeding-regulating peptides gene expressions in serotonin depleted rats2014
Author(s)
Yoshimura M, Hagimoto M, Matsuura T, Ohkubo J, Ohno M, Maruyama T, Ishikura T, Hashimoto H, Kakuma T, Yoshimatsu H, Terawaki K, Uezono Y, Toyohira Y, Yanagihara N, Ueta Y
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Journal Title
J Physiol Sci
Volume: 64
Pages: 97-104
DOI
Peer Reviewed
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[Journal Article] Stimulatory effect of nobiletin, a citrus polymethoxy flavone, on catecholamine synthesis through Ser19 and Ser 40 phosphorylation of tyrosine hydroxylase in cultured bovine adrenal medullary cells2014
Author(s)
Zhang H, Yanagihara N, Toyohira Y, Takahashi K, Inagaki H, Satoh N, Li X, Goa X, Tsutsui M, Takahaishi K
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Journal Title
Naunyn Schmiedebergs Arch Pharmacol.
Volume: 387
Pages: 15-22
DOI
Peer Reviewed
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[Journal Article] Crucial vasculoprotective role of the whole nitric oxide synthase system in vascular lesion formation in mice : Involvement of bone marrow-derived cells2011
Author(s)
Furuno Y, Morishita T, Toyohira Y, Yamada S, Ueno S, Morisada N, Sugita K, Noguchi K, Sakanashi M, Miyata H, Tanimoto A, Sasaguri Y, Shimokawa H, Otsuji Y, Yanagihara N, Tamura M, Tsutsui M
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Journal Title
Nitric Oxide
Volume: 25
Pages: 350-9
DOI
Peer Reviewed
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