2013 Fiscal Year Final Research Report
Basic analysis for evaluating the possibility of phytochemicals as a therapeutic and prophylactic agent for mood disorders
| Project/Area Number |
23617036
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrated Nutrition Science
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SAKAMAKI Ruka 西南女学院大学, 保健福祉学部, 准教授 (80389486)
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| Project Period (FY) |
2011 – 2013
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| Keywords | フィトケミカル / ポリフェノール / 気分障害 / カテコールアミン / ノルアドレナリントランスポーター / セロトニントランスポーター / チロシン水酸化酵素 |
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| Research Abstract |
Resveratrol has attracted wide attention because of its antioxidant, anti-apoptotic and anti-inflammatory effects. We have previously reported that resveratrol inhibits acetylcholine-induced catecholamine secretion and synthesis through suppressing ion influx in cultured bovine adrenal medullary cells. In this study, we examined the effect of resveratrol on noradrenaline (NA) transporter, which is also known to be the cellular targets of antidepressants. Resveratrol stimulated NA uptake by SK-N-SH cells at low concentration, it was inhibited NA uptake at high concentration against this. Kinetics analysis of the effect of resveratrol on NA transport in SK-N-SH cells indicated that they decreased the Vmax of NA transport with little or no change in Km. Scatchard analysis of nisoxetine binding to plasma membranes showed that resveratrol did not affect the Bmax and the Kd.
The present findings suggest that resveratrol plays a biphasic role in the regulation of neuronal NA transport.
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Author(s)
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Journal Title
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DOI
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Author(s)
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Journal Title
Naunyn Schmiedebergs Arch Pharmacol.
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DOI
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Journal Title
Nitric Oxide
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Pages: 350-9
DOI
Peer Reviewed
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