2013 Fiscal Year Final Research Report
Transcriptome analysis of cardiac progenitors to identify small RNAs that induce transdifferentiation
| Project/Area Number |
23618009
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Regenerative medicine
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| Research Institution | The University of Kitakyushu |
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Principal Investigator |
HIDAKA KYOKO 北九州市立大学, 基盤教育センター, 教授 (00216681)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAZAWA Koji 北九州市立大学, 国際環境工学部, 教授 (00304733)
SAKURAI Kazuo 北九州市立大学, 国際環境工学部, 教授 (70343431)
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| Project Period (FY) |
2011 – 2013
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| Keywords | miRNA / 分化転換 / 心筋細胞 / 多能性幹細胞 |
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| Research Abstract |
In mammalian heart, cardiac muscle cells (cardiomyocytes) are terminally differentiated and their regenerative capacity is limited. Recent studies have demonstrated that a combination of transcription factors could reprogram fibroblast directly to cardiomyocyte-like cells, albeit at a low efficiency. On the other hand, microRNAs (miRNAs), a family of small RNAs, have also been shown to convert cell fate by regulating gene expression. Here, we identified miRNAs that were expressed in cardiac progenitor cells, by transcriptome analysis of murine ES cell-derived cells. We also found that a combination of these miRNAs could induce myocyte-specific genes in cardiac fibroblasts. These findings may improve direct reprogramming methods towards regenerative cardiac therapy.
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