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2013 Fiscal Year Final Research Report

Safety and effectiveness of neural stem cells directly induced from skin fibroblasts

Research Project

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Project/Area Number 23618010
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Regenerative medicine
Research InstitutionKeio University

Principal Investigator

AKAMATSU WADO  慶應義塾大学, 医学部, 講師 (60338184)

Project Period (FY) 2011 – 2013
Keywords神経幹細胞 / ダイレクトリプログラミング
Research Abstract

We succeeded in making human directly induced neural stem cells by using Sendai virus vectors. Although we were able to obtain increased number of neurospheres compared to retroviral vectors, SeV-induced neurospheres did not differentiate into neural cells efficiently.

  • Research Products

    (34 results)

All 2014 2013 2012 2011 2009

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (17 results) (of which Invited: 16 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Involvement of ER Stress in Dysmyelination of Pelizaeus-Merzbacher Disease with PLP1 Missense Mutations Shown by iPSC-Derived Oligodendrocytes2014

    • Author(s)
      Numasawa-Kuroiwa Y, Okada Y, Shibata S, Kishi N, Akamatsu W, Shoji M, Nakanishi A, Oyama M, Oasaka H, Inoue K, Takahashi K, Yamanaka S, Kosaki K, Takahashi T, Okano H
    • Journal Title

      Stem Cell Rep

      Volume: 2 Pages: 1-14

    • DOI

      10.1016/j.stemcr.2014.03.007

    • Peer Reviewed
  • [Journal Article] Reprogramming non-human primate somatic cells into functional neuronal cells by defined factors2014

    • Author(s)
      Zhou Z, Kohda K, Ibata K, Kohyama J, Akamatsu W, Yuzaki M, Okano HJ, Sasaki E, Okano H
    • Journal Title

      Mol Brain

      Volume: 7(1) Pages: 24

    • DOI

      10.1186/1756-6606-7-24

    • Peer Reviewed
  • [Journal Article] Prenatal deletion of the RNA binding protein HuD disrupts postnatal cortical circuit maturation and behavior2014

    • Author(s)
      DeBoer E, Azevedo R, Vega T, Brodkin J, Akamatsu W, Okano H, Wagner G, Rasin MR
    • Journal Title

      J Neurosci

      Volume: 34(10) Pages: 3674-86

    • DOI

      10.1523/JNEUROSCI.3703-13

    • Peer Reviewed
  • [Journal Article] The RNA binding protein, HuD regulates autophagosome formation in pancreatic βcells by promoting autophagy-related gene 5 expression2014

    • Author(s)
      Kim C, Kim W, Lee H, Ji E, Choe YJ, Martindale JL, Akamatsu W, Okano H, Kim HS, Nam SW, Gorospe M, Lee EK
    • Journal Title

      J Biol Chem

      Volume: 289(1) Pages: 112-21

    • DOI

      10.1074/jbc.M113.474700

    • Peer Reviewed
  • [Journal Article] Increased Ll Retrotransposition in the neuronal genome in Schizo phrenia2014

    • Author(s)
      Bundo M, Toyoshima M, Ueda J, Nemoto-Miyake T, Sunaga F, Toritsuka M, Ikawa D, Kakita A, Okada Y, Akamatsu W, Kato M, Okano H, Kasai K, Kishimoto T, Nawa H, Yoshikawa T, Kato T, Iwamoto K
    • Journal Title

      Neuron

      Volume: 81(2) Pages: 306-13

    • DOI

      10.1016/j.neuron.2013.10.053

    • Peer Reviewed
  • [Journal Article] A human Dravet syndrome model from patient induced pluripotent stem cells2013

    • Author(s)
      Higurashi N, Uchida T, Christoph L, Misumi Y, Okada Y, Akamatsu W, Imaizumi Y, Zhang B, Nabeshima K, Mori M, Katsurabayashi S, Shirasaka S, Okano H and Hirose S
    • Journal Title

      Mol Brain

      Volume: 6(1) Pages: 19

    • DOI

      10.1186/1756-6606-6-19

    • Peer Reviewed
  • [Journal Article] Generation of human melanocytes from induced pluripotentstem cells2013

    • Author(s)
      Ohta S, Imaizumi Y, Akamatsu W, Okano H, Kawakami Y
    • Journal Title

      Methods Mol Biol

      Volume: 989 Pages: 193-215

    • DOI

      10.1007/978-1-62703-330-5_16

    • Peer Reviewed
  • [Journal Article] Enhanced Aggregation of Androgen Receptor in Induced Pluripotent Stem Cell-derived Neurons from Spinal and Bulbar Muscular Atrophy2013

    • Author(s)
      Nihei Y, Ito D, Okada Y, Akamatsu W, Yagi T, Yoshizaki T, Okano H, Suzuki N
    • Journal Title

      J B iol Chem

      Volume: 288(12) Pages: 8043-52

    • DOI

      10.1074/jbc.M112.408211

    • Peer Reviewed
  • [Journal Article] Human induced pluripotent stem cell-derived ectodermal precursor cells contribute to hair follicle morphogenesis in vivo2013

    • Author(s)
      Veraitch O, Kobayashi T, Imaizumi Y, Akamatsu W, Sasaki T, Yamanaka S, Amagai M, Okano H and Ohyama M
    • Journal Title

      J Invest Dermatol

    • DOI

      10.1038/jid

    • Peer Reviewed
  • [Journal Article] Mitochondrial dysfunction associated with increased oxidative stress and alpha-synuclein accumulation in PARK2 iPSC-derived neurons and postmortem brain tissue2012

    • Author(s)
      Imaizumi Y, Okada Y, Akamatsu W, Koike M, Kuzumaki N, Hayakawa H, Nihira T, Kobayashi T, Ohyama M, Sato S, Takanashi M, Funayama M, Hirayama A, Soga T, Hishiki T, Suematsu M, Yagi T, Ito D, Kosakai A, Hayashi K, Shouji M, Nakanishi A, Suzuki N, Mizuno Y, Mizushima N, Amagai M, Uchiyama Y, Mochizuki H, Hattori N, Okano H
    • Journal Title

      Mol Brain

      Volume: 5(1) Pages: 35

    • DOI

      10.1186/1756-6606-5-35

    • Peer Reviewed
  • [Journal Article] Regeneration of the damaged central nervous system through reprog ramming technology : Basic concepts and potential application for cell replacement therapy2012

    • Author(s)
      Matsui T, Akamatsu W, Nakamura M, Okano H
    • Journal Title

      Exp Neurol

    • DOI

      10.1016/j.expneurol

    • Peer Reviewed
  • [Journal Article] Derivation of induced pluripotent stem cells by retroviral gene transduction in mammalian species2012

    • Author(s)
      Imamura M, Okuno H, Tomioka I, Kawamura Y, Lin ZY, Nakajima R, Akamatsu W, Okano HJ, Matsuzaki Y, Sasaki E, Okano H
    • Journal Title

      Methods Mol Biol

      Volume: 925 Pages: 21-48

    • DOI

      10.1007/978-1-62703-011-3_2

    • Peer Reviewed
  • [Journal Article] Establishment of induced pluripotentstem cells from centenarians for neurodegenerative disease research2012

    • Author(s)
      Yagi T, Kosakai A, Ito D, Okada Y, Akamatsu W, Nihei Y, Nabetani A, Ishikawa F, Arai Y, Hirose N, Okano H, Suzuki N
    • Journal Title

      PLoS One

      Volume: 7(7) Pages: e41572

    • DOI

      10.1371/journal.pone.0041572

    • Peer Reviewed
  • [Journal Article] Neuralstem cells directly differentiated from partially reprogrammed fibroblasts rapidly acquire gliogenic competency2012

    • Author(s)
      Matsui T, Takano M, Yoshida K, Ono S, Fujisaki C, Matsuzaki Y, Toyama Y, Nakamura M, Okano H, Akamatsu W
    • Journal Title

      Stem Cells

      Volume: 30(6) Pages: 1109-19

    • DOI

      10.1002/stem.1091

    • Peer Reviewed
  • [Journal Article] RNA-binding protein HuD controls insulin translation2012

    • Author(s)
      Lee EK, Kim W, Tominaga K, Martindale JL, Yang X, Subaran SS, Carlson OD, Mercken EM, Kulkarni RN, Akamatsu W, Okano H, Perrone-Bizzozero NI, de Cabo R, Egan JM, Gorospe M
    • Journal Title

      Mol Cell

      Volume: 45 (6) Pages: 826-35

    • DOI

      10.1016/j.molcel.2012.01.016

    • Peer Reviewed
  • [Journal Article] Modeling familial Alzheimer's disease with induced pluripotentstem cells2011

    • Author(s)
      Yagi T, Ito D, Okada Y, Akamatsu W, Nihei Y, Yoshizaki T, Yamanaka S, Ok ano H, Suzuki N
    • Journal Title

      Hum Mol Genet

      Volume: 20(23) Pages: 4530-9

    • Peer Reviewed
  • [Presentation] 多能性幹細胞(ES細胞/iPS細胞)を用いた神経疾患の再生医療と病態研究2014

    • Author(s)
      赤松和土
    • Organizer
      順天堂大学第136回お茶の水木曜会
    • Place of Presentation
      東京
    • Year and Date
      2014-03-13
    • Invited
  • [Presentation] Direct Reprogrammingの最近の進歩:神経幹細胞の直接誘導とその応用2014

    • Author(s)
      赤松和土
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都(シンポジウム)
    • Year and Date
      2014-03-05
    • Invited
  • [Presentation] iPS細胞技術を用いた神経疾患研究と治療2013

    • Author(s)
      赤松和土
    • Organizer
      第37回日本血液事業学会総会
    • Place of Presentation
      札幌(シンポジウム)
    • Year and Date
      2013-10-21
    • Invited
  • [Presentation] iPS technology-based regenerative medicine for damaged central nervous system2013

    • Author(s)
      赤松和土
    • Organizer
      第11回遺伝子治療学会
    • Place of Presentation
      岡山(シンポジウム)
    • Year and Date
      2013-07-06
    • Invited
  • [Presentation] 疾患iPS細胞と創薬:「神経疾患患者由来細胞からの神経系細胞の誘導と病態解析」2013

    • Author(s)
      赤松和土
    • Organizer
      炎症再生学会
    • Place of Presentation
      京都(シンポジウム)
    • Year and Date
      2013-07-02
    • Invited
  • [Presentation] iPS細胞を使った神経疾患の治療法の開発2013

    • Author(s)
      赤松和土
    • Organizer
      第20回東京血管疾患研究所セミナー
    • Place of Presentation
      東京
    • Year and Date
      2013-06-08
    • Invited
  • [Presentation] iPS細胞技術を用いた神経系の再生医療と疾患解析2013

    • Author(s)
      赤松和土
    • Organizer
      第2回小児泌尿器科研究会
    • Place of Presentation
      東京
    • Year and Date
      2013-06-01
    • Invited
  • [Presentation] 多能性幹細胞を用いた神経疾患の再生医療と病態研究2013

    • Author(s)
      赤松和土
    • Organizer
      第11回横浜小児先端医療セミナー
    • Place of Presentation
      横浜
    • Year and Date
      2013-05-24
    • Invited
  • [Presentation] iPS細胞技術の神経疾患研究・治療への応用2013

    • Author(s)
      赤松和土
    • Organizer
      第116回日本小児科学会学術集会
    • Place of Presentation
      広島
    • Year and Date
      2013-04-19
    • Invited
  • [Presentation] 45 iPS細胞技術を用いた中枢神経系の再生と創薬研究:iPS technology-based cell therapy for damaged CNS and investigation of neural disorders2013

    • Author(s)
      赤松和土,岡野栄之
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京(シンポジウム)
    • Year and Date
      2013-03-29
    • Invited
  • [Presentation] 疾患特異的iPS細胞を用いた病態解明および治療法確立研究:神経疾患患者由来細胞からの神経系細胞の誘導と病態解析2013

    • Author(s)
      赤松和土
    • Organizer
      日本再生医療学会総会
    • Place of Presentation
      東京(シンポジウム)
    • Year and Date
      2013-03-22
    • Invited
  • [Presentation] 多能性幹細胞・神経幹細胞を用いた再生医療と病態解析2013

    • Author(s)
      赤松和土
    • Organizer
      愛知医科大学細胞治療研究会
    • Place of Presentation
      愛知
    • Year and Date
      2013-03-05
    • Invited
  • [Presentation] ALSに対する再生医療の開発:幹細胞生物学を応用した神経疾患病態研究2012

    • Author(s)
      赤松和土
    • Organizer
      第53回日本神経学会学術大会
    • Place of Presentation
      東京
    • Year and Date
      2012-05-23
    • Invited
  • [Presentation] 多能性幹細胞から神経幹細胞を生み出す分子機構とその応用2012

    • Author(s)
      赤松和土
    • Organizer
      第12回日本分子生物学会春季シンポジウム
    • Place of Presentation
      山梨
    • Year and Date
      2012-04-26
    • Invited
  • [Presentation] 13基礎研究セミナー:多能性幹細胞由来神経幹細胞を用いた神経系の再生医療の展望2012

    • Author(s)
      赤松和土
    • Organizer
      第116回日本眼科学会
    • Place of Presentation
      東京(シンポジウム)
    • Year and Date
      2012-04-06
    • Invited
  • [Presentation] "ヒト"をキーワードにした多能性幹細胞研究の展開:体細胞からの安全な神経幹細胞の直接誘導2012

    • Author(s)
      赤松和土
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      長野(シンポジストおよび座長)
    • Year and Date
      2012-03-31
    • Invited
  • [Presentation] 多能性幹細胞を用いた神経系の再生医療の展望2011

    • Author(s)
      赤松和土,岡野栄之
    • Organizer
      長野県医学会例会
    • Place of Presentation
      長野(特別講演)
    • Year and Date
      2011-07-25
  • [Patent(Industrial Property Rights)] 神経幹細胞製造方法2009

    • Inventor(s)
      岡野栄之、赤松和土
    • Industrial Property Rights Holder
      岡野栄之、赤松和土
    • Industrial Property Rights Type
      PCT
    • Industrial Property Number
      PCT/JP2009/005856
    • Filing Date
      2009-11-04
    • Overseas

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Published: 2015-07-16  

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