2013 Fiscal Year Final Research Report
Quality control of iPS cells by studying differentiation resistance.
| Project/Area Number |
23618011
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Regenerative medicine
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| Research Institution | Keio University |
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Principal Investigator |
NAGAMATSU Go 慶應義塾大学, 医学部, 助教 (70453545)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 幹細胞 / 多能性 / リプログラミング |
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| Research Abstract |
A tagging system was developed to sort induced pluripotent stem (iPS) cells according to the expression levels of each of the four reprogramming factors. Using this system, the effective ratio (Oct3/4-high, Sox2-low, Klf4-high, c-Myc-high) was. To investigate the molecular basis, microarray analysis was performed. Pathway analysis revealed that the G protein-coupled receptor (GPCR) pathway was up-regulated significantly under the high efficiency condition and treatment with the chemokine, C-C motif ligand 2, a member of the GPCR family, enhanced somatic cell reprogramming. Furthermore, the genetic modifier, Whsc1l1 (variant 1), also improved the efficiency of somatic cell reprogramming.
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[Journal Article] Induction of pluripotent stem cells from primordial germ cells by single reprogramming factors2013
Author(s)
Nagamatsu G, Kosaka T, Saito S, Honda H, Takubo K, Kinoshita T, Akiyama H, Sudo T, Horimoto K, Oya M, Suda T
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Journal Title
Stem Cells
Volume: 31(3)
Pages: 479-87
DOI
Peer Reviewed
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