2012 Fiscal Year Final Research Report
Live imaging of energy supply for synaptic protein transport
| Project/Area Number |
23650169
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neuroscience in general
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 分子 / 細胞神経科学 / ミトコンドリア / 軸索輸送 / シナプス |
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| Research Abstract |
Preparing synaptic contacts in developing (three weeks in culture) and mature (6 weeks in culture) superior cervical ganglion (SCG) neurons, I performed live image of axonal transport of CFP-labeled mitochondria, RFP-labeled synaptophysin,a synaptic vesicle protein, and GFP-labeled CAST, an active zone protein, applying variousaction potential firing pattern to a presynaptic neuron. Their conduction velocity in the axon and their accumulation in the axon or into the terminal were impaired with dysfunction of a motor adaptor protein.
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[Journal Article] Ca^(2+)- independent Activation of Ca^(2+)/Calmodulin-dependent Protein Kinase II Bound to the C-terminal Domain of CaV2.1 Channels2013
Author(s)
Magupalli, V.G., Mochida, S., Jiang, X. Westenbroek, R.E., Nairn, A.C., Scheuer, T. & Catterall, W.A
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Journal Title
J. Biol. Chem
Volume: 288(7)
Pages: 637-48
DOI
Peer Reviewed
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