Optogenetic approach of gene regulation

2011 Fiscal Year Final Research Report

• Project/Area Number: 23650172
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neuroscience in general
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: HAYASHI Yasunori 独立行政法人理化学研究所, 記憶メカニズム研究チーム, チームリーダー (90466037)
• Project Period (FY): 2011
• Keywords: 遺伝子発現 / Cre / LOVドメイン / 光遺伝学

Research Abstract

In order to generate photoactivatable Cre, we made a series of fusion proteins between LOV domain and Cre and introduce into HEK cells along with lox-stop-lox-GFP, which allows detection of Cre activity by GFP. However, irrespective illumination, we saw GFP signal, indicating that LOV domain did not effectively suppress the activity of Cre. However, in some of constructs, GFP was dimmer than others. We will further pursue these constructs to see if we can improve them by adding additional mutations.

Research Products

• [Journal Article] A rapid and effective FISH protocol with hybridization-sensitive fluorescent linear oligo probes (2012)
  • Author(s): Wang, D. O., Matsuno, H., Ikeda, S., Nakamura, A., Yanagisawa, H., Hayashi, Y., Okamoto, A
  • Journal Title: RNA Volume: 18 Pages: 166-175
  • Peer Reviewed
• [Journal Article] Experience-dependent regulation of CaMKII activity within single visual cortex synapses in vivo (2011)
  • Author(s): Mower, A. F., Kwok, S. M., Yu, H., Majewska, A. K., Okamoto, K.-I., Hayashi, Y., Sur, M.
  • Journal Title: Proc. Natl. Acad. Sci Volume: 108 Pages: 21241-21246
  • Peer Reviewed
• [Journal Article] Structural plasticity of dendritic spines (2011)
  • Author(s): Bosch, M., Hayashi, Y
  • Journal Title: Curr. Opin. Neurobiol
  • DOI: doi:10.1016/j.conb.2011.09.002
  • Peer Reviewed
• [Journal Article] The Ca2+and Rho GTPase signaling pathways underlying activity-dependent actin remodeling at dendritic spines
  • Author(s): Saneyoshi, T., Hayashi, Y
  • Journal Title: Cytoskeleton
  • DOI: doi:10.1002/cm.21037
  • Peer Reviewed
• [Presentation] The role of CaMKII beta in modulating dendritic spine structural plasticity (2011)
  • Author(s): Lakhanpal, G., Kim, K., Kato-Hayashi, M., Hayashi, Y., Okamoto, K
  • Organizer: SfN2011
  • Place of Presentation: Washington DC
  • Year and Date: 2011-11-16
• [Presentation] CaMKII gates rapid structural plasticity in hippocampal dendritic spines (2011)
  • Author(s): Kim, K., Hayashi, M., Narayanan, R., Suzuki, A., Matsuura, K., Okamoto, K., Hayashi, Y.
  • Organizer: SfN2011
  • Place of Presentation: Washington DC
  • Year and Date: 2011-11-16
• [Presentation] A quantitative anyalysis of AMPA receptor GluR1 phospho-isotypes (2011)
  • Author(s): Hosokawa, T., Hayashi, Y.
  • Organizer: SfN2011
  • Place of Presentation: Washington DC
  • Year and Date: 2011-11-15
• [Presentation] Structural roles of CaMKII (2011)
  • Author(s): Hayashi, Y.
  • Organizer: 日本神経化学会
  • Place of Presentation: 金沢
  • Year and Date: 2011-09-26
• [Presentation] In vivo two-photon imaging of neuronal circuit activity using transgenic mice that express fluorescent calcium sensor proteins in the brain (2011)
  • Author(s): Sato, M., Kawano, M., Islam, T., Yamaguchi, Y., Ohkura, M., Nakai, J., Hayashi, Y.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-16
• [Presentation] A quantitative analysis of AMPA receptor GluR1 phospho-isotypes (2011)
  • Author(s): Hosokawa, T., Hayashi, Y.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-15
• [Presentation] The regulation of dendritic spine structural plasticity by CaMKII beta (2011)
  • Author(s): Lakhanpal, G. K., Kim, K., Kato-Hayashi, M., Hayashi, Y.,& Okamoto, K
  • Organizer: 8th IBRO World Congress of Neuroscience
  • Place of Presentation: Florence, Italy
  • Year and Date: 2011-07-15
• [Presentation] Phosphoisotype analysis of AMPA receptor GluR1 (2011)
  • Author(s): Hayashi, Y.
  • Organizer: Gordon Research Conference Excitatory Synapses& Brain Function
  • Place of Presentation: Easton, MA, USA
  • Year and Date: 2011-06-29
• [Book] Roles of neuronal activity-induced gene products in Hebbian and homeostatic synaptic plasticity, tagging and capture (2012)
  • Author(s): Hayashi, Y., Okamoto, M., Bosch, M., Futai, Y
  • Publisher: Springer-Verlag