Development of a viral vector towards the quantitive analysis of the input-output characteristics which a single nerve cell shows

2012 Fiscal Year Final Research Report

• Project/Area Number: 23650175
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Kyoto University
• Principal Investigator: KANEKO Takeshi 京都大学, 医学研究科, 教授 (90177519)
• Co-Investigator(Kenkyū-buntansha): HIOKI Hiroyuki (00402850)
• Project Period (FY): 2011 – 2012
• Keywords: レンチウイルス / アデノ随伴ウイルス / 神経細胞 / 局所神経回路 / 定量的解析

Research Abstract

1In the present study, we developed viral vectors, which express transsynaptic tracer protein, tetanus toxin C fragment (TTC) or wheat germ agglutinin (WGA). We then injected the viral vectors into the neocortex of the wild rats or Cre Tg mice, and observed the immunoreactivity for TTC or WGA. We could observe the transsynaptic transfer of TTC or WGA, but unfortunately the signal intensities were too week to analyze the cell types and numbers even with high-sensitive detection method.

Research Products

• [Journal Article] Correlative analysis of immunoreact- ivityin confocal laserscanning microscopy andion beam milling (2013)
  • Author(s): Sonomura T, Furuta F, Nakatani I, Yamamoto Y, Unzai T, Matsuda W, Iwai h
  • Journal Title: Frontiers in Neural Circuits Volume: vol. 7, article 26
  • DOI: DOI:10.3389/fncir.2013.00026
  • Peer Reviewed
• [Journal Article] Differential expression of VGLUT1 or VGLUT2 in thetrigeminothala- Mic or trigeminocerebellar projection neurons in the rat (2013)
  • Author(s): Ge S-N, Li Z-H, Tang J, Ma Y, Hioki H, Zhang T, Lu Y-C, Zhang F-X, Mizuno N, Li J-L
  • Journal Title: Brain Structure and Function Volume: (in press)
  • DOI: DOI10.1007/s00429-012-0495-1
  • Peer Reviewed
• [Journal Article] A quantitative analysis of axon bouton distribution of subthalamic nucleus neurons in the rat by vector (2013)
  • Author(s): Koshimizu Y, Fujiyama F, Nakamura KC, Furuta T, Kaneko T
  • Journal Title: The Journal of Comparative Neurology Volume: vol. 521(no. 9) Pages: 2125-2146
  • DOI: DOI10.1002/cne.23277
  • Peer Reviewed
• [Journal Article] Cell type-specific inhibitory inputs to dendritic and somatic compartments of parvalbumin-expressing neocortical interneuron (2013)
  • Author(s): Hioki H, Okamoto S, Konno M, Kameda H, Sohn J, Kuramoto E, Fujiyama F, Kaneko T
  • Journal Title: The Journal of Neuroscience Volume: vol. 33 (no. 2) Pages: 544-555
  • DOI: DOI:10.1523/JNEUROSCI.2255-12.2013
  • Peer Reviewed
• [Journal Article] Parvalbuminproducing cortical interneurons receive inhibitory inputs on proximal portions and cortical excitatory inputs on distal dendrites (2012)
  • Author(s): Kameda H, Hioki H, Tanaka YH, Tanaka T, Sohn J, Sonomura T, Furuta T,Fujiyama F, Kaneko T
  • Journal Title: European Journal of Neuroscience Volume: vol. 35 (no. 6) Pages: 834-854
  • DOI: DOI:10.1111/j.1460-9568.2012.08027.x
  • Peer Reviewed
• [Journal Article] Morphological analysis of thalamocortical axon fibers of rat posterior thalamic nuclei: A single neuron tracing study with viral vectors (2012)
  • Author(s): Ohno S, Kuramoto E, Furuta T, Hioki H, Tanaka YR, Fujiyama F, Sonomura T, Uemura M, Sugiyama K, Kaneko T
  • Journal Title: Cerebral Cortex Volume: vol. 22 Pages: 2840-2857
  • DOI: DOI:10.1093/cercor/bhr356
  • Peer Reviewed
• [Journal Article] Local connections of excitatory neurons to corticothalamic neurons in the rat barrel cortex (2011)
  • Author(s): Tanaka YR, Tanaka YH, Konno M, Fujiyama F, Sonomura T, Okamoto-Furuta K, Kameda H, Hioki H, Furuta T, Nakamura KC, Kaneko T
  • Journal Title: Journal of Neuroscience Volume: vol. 31 (no. 50) Pages: 18223-28236
  • DOI: DOI:10.1523/JNEUROSCI.3139-11.2011
  • Peer Reviewed
• [Journal Article] Local connections of layer 5 GABAergic interneurons to corticospinal neurons (2011)
  • Author(s): Tanaka YH, Tanaka YR, Fujiyama F, Furuta T, Yanagawa Y, Kaneko T
  • Journal Title: Frontier in Neural Circuits Volume: vol. 5, article 12
  • Peer Reviewed
• [Journal Article] Local connections of excitatory neurons inmotor-associated cortical areas of the rat
  • Author(s): Kaneko T
  • Journal Title: Frontier in Neural Circuits Volume: (in press)
  • DOI: DOI:10.3389/fncir.2013.00075
  • Peer Reviewed
• [Presentation] Optogenetic stimulation of neurons in the preoptic area inhibits metabolic heat production. オプトジェネティクスを用いた視索前野ニューロンの刺激による代謝性熱産生の抑制 (2013)
  • Author(s): 中村佳子、日置寛之、片岡直也、金子武嗣、中村和弘
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: タワーホール船堀 東京
  • Year and Date: 20130327-29
• [Presentation] Preprodynorphin-producing interneurons constitute a subpopulation of somatostatin-positive neurons in the mouse neocortex 大脳皮質ソマトスタチン陽性抑制性細胞の一部はプレプロダイノルフィンを産生する (2013)
  • Author(s): 孫在隣、日置寛之、岡本慎一郎、金子武嗣
  • Organizer: 第118回 日本解剖学会・全国学術集会
  • Place of Presentation: 高松
  • Year and Date: 2013-03-29
• [Presentation] Excitatory and Inhibitory Inputs to Parvalbumin-Expressing Interneurons in the Mouse Primary Motor Cortex パルブアルブミン発現皮質神経細胞に対する興奮性・抑制性入力様式を定量的に解析する (2013)
  • Author(s): 日置寛之、王濤、孫在隣、岡本慎一郎、亀田浩司、倉本恵梨子、藤山文乃、金子武嗣
  • Organizer: 第118回 日本解剖学会・全国学術集会
  • Place of Presentation: 高松
  • Year and Date: 2013-03-28
• [Presentation] 運動系視床皮質投射の神経回路 (2013)
  • Author(s): 倉本恵梨子、藩世秀、大野幸、田中康裕、雲財知、古田貴寛、日置寛之、中村公一、藤山文乃、金子武嗣
  • Organizer: 第118回 日本解剖学会・全国学術集会
  • Place of Presentation: 高松
  • Year and Date: 2013-03-28
• [Presentation] Cell type-specific inhibitory inputs to dendritic and somatic compartments of parvalbumin-expressing neocortical interneuron. (2012)
  • Author(s): Hioki H, Okamoto S, Konno M, Kameda H, Sohn J, Kuramoto E, Fujiyama F, T. Kaneko T
  • Organizer: The 42nd Society for Neuroscience Annual Meeting
  • Place of Presentation: New Orleans, MI, USA
  • Year and Date: 20121013-17
• [Presentation] Preprodynorphin-producing neocortical interneurons constitute a subpopulation of somatostatin-expressing neurons (2012)
  • Author(s): Sohn J, Hioki H, Okamoto S, Kaneko T
  • Organizer: The 42nd Society for Neuroscience Annual Meeting
  • Place of Presentation: New Orleans, MI, USA
  • Year and Date: 20121013-17
• [Presentation] Optogenetic stimulation of medullary rapheprojecting axons of dorsomedial hypothalamic neurons elicits thermo- genic and cardiovascular responses (2012)
  • Author(s): Nakamura K, Kataoka N, Hioki H, Kaneko, T
  • Organizer: Optogenetics and Pharmacogenetics in Neuronal Function and Dysfunction
  • Place of Presentation: Hilton Riverside, New Orleans, MI, USA.
  • Year and Date: 2012-10-11
• [Presentation] The loss of mDia, a Rho effector and actin nucleator, disrupts Ephrin-mediated axon guidance, leading to defective left-right limb alternation during locomotion (2012)
  • Author(s): 豊田洋輔、篠原亮太、タムケオ ディーン 上條博史、西丸広史、日置寛之、金子武嗣、石崎敏理、古屋敷智之、成宮周
  • Organizer: 第35回日本神経科学学会(Neuro2012)
  • Place of Presentation: 名古屋国際会議場 名古屋
  • Year and Date: 2012-09-21
• [Presentation] Single-neuron tracing study of thalamocortical projections arising from the rat mediodorsal nucleus by using a Sindbis viral vector. ラット視床MD核から大脳皮質への軸索投射を単一ニューロンレベルで形態解析する:シンドビスウイルスベクターを用いて (2012)
  • Author(s): 倉本恵梨子、藩世秀、古田貴寛、日置寛之、金子武嗣
  • Organizer: 第35回日本神経科学学会(Neuro2012)
  • Place of Presentation: 名古屋国際会議場 名古屋
  • Year and Date: 2012-09-18
• [Presentation] Activation of Catecholaminergic Nuclei in the Brainstem of the Rat by Acute Sustained Hypoxia (2012)
  • Author(s): Mandy Siu Yu Lung, Hiroyuki Hioki, Takeshi kaneko
  • Organizer: 第35回日本神経科学学会(Neuro2012)
  • Place of Presentation: 名古屋国際会議場 名古屋
  • Year and Date: 2012-09-18
• [Remarks]
  • URL: http://www.mbs.med.kyoto-u.ac.jp