2012 Fiscal Year Final Research Report
Development of screening method to isolate molecules that regulate behaviors of neurotransmitter receptors at synaptic membranes
| Project/Area Number |
23650180
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
TABUCHI Katsuhiko 生理学研究所, 大脳皮質機能研究系, 教授(兼任) (20546767)
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| Co-Investigator(Renkei-kenkyūsha) |
EGASHIRA Yoshihiro 同志社大学, 脳科学研究科, 研究員 (80582410)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 神経実験形態学 / シナプス / 凍結割断レプリカ免疫電顕 / 子宮内エレクトロポレーション |
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| Research Abstract |
SDS-digested freeze-fracture replica labeling electronmiroscopy (SDS-FRL EM) is a technique that enables visualization of proteins, such as neurotransmitter receptors, on synapse membrane with maintenance of space structure. However, the technique in combination with gene transfection has not been established because of the difficulty in recognition of transfected neurons. In this project, we developed a new technique to distinguish transfected neurons on replica membranes by refolding membrane tethered fluorescent proteins that are introduced as markers after SDS digestion.
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[Journal Article]2013
Author(s)
Elaine C. Budreck, Oh-Bin Kwon, Jung Hoon Jung, Stephane Baudouin, Albert Thommen, Hye-Sun Kim, Yugo Fukazawa, Harumi Harada, Katsuhiko Tabuchi, Ryuichi Shigemoto, Peter Scheiffele
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Journal Title
Proc Natl Acad Sci USA
Volume: 110(2)
Pages: 725-730
DOI
Peer Reviewed
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