Analysis of non-coding RNAs regulating neural differentiation of human ES cells.

2012 Fiscal Year Final Research Report

• Project/Area Number: 23650194
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Keio University
• Principal Investigator: OKADA Yohei 慶應義塾大学, 医学部, 特任講師 (30383714)
• Co-Investigator(Renkei-kenkyūsha): TSUNODA Tatsuhiko 理化学研究所, ゲノム医科学研究センター, チームリーダー (10273468) ; MIYA Fuyuki 理化学研究所, ゲノム医科学研究センター, 研究員 (50415311)
• Project Period (FY): 2011 – 2012
• Keywords: ヒトES細胞 / ノンコーディングRNA

Research Abstract

Several non-coding RNAs (ncRNAs) upregulated during neural differentiation of human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) were identified by genome-wide transcriptome analysis. In addition, methods for the analysis of iden

Research Products

• [Journal Article] Steps toward safe cell therapy using induced pluripotent stem cells (2013)
  • Author(s): Okano H, Nakamura M, Yoshida K, Okada Y, Tsuji O, Nori S, Ikeda E, Yamanaka S, Miura K
  • Journal Title: Circulation Research Volume: 112(3) Pages: 523-33
  • DOI: DOI:10.1161/CIRCRESAHA.111.256149
  • Peer Reviewed
• [Journal Article] Enhanced aggregation of androgen receptor in induced pluripotent stem cell-derived neurons from spinal and bulbar muscular atrophy (2013)
  • Author(s): Nihei Y, Ito D, Okada Y, Akamatsu W, Yagi T, Yoshizaki T, Okano H, Suzuki N
  • Journal Title: J Biol Chem Volume: 288(12) Pages: 8043-52
  • DOI: DOI:10.1074/jbc.M112.408211
  • Peer Reviewed
• [Journal Article] Pre-Evaluated Safe Human iPSC-DerivedNeural Stem Cells Promote Functional Recovery after Spinal Cord Injury in Common Marmoset without Tumorigenicity (2012)
  • Author(s): Kobayashi Y, Okada Y, Itakura G, Iwai H, Nishimura S, Nori S, Hikishima K, Konomi T, Fujiyoshi K, Tsuji O, Toyama Y, Yamanaka S, Nakamura M, Okano H
  • Journal Title: PLoS One Volume: 7(12)
  • DOI: DOI:10.1371/journal.pone.0052787
  • Peer Reviewed
• [Journal Article] Multiple analyses of G-protein coupled receptor (GPCR) expression in the development of gefitinib-resistance in transforming non-small-cell lung cancer (2012)
  • Author(s): Kuzumaki N, Suzuki A, Narita M, Hosoya T, Nagasawa A, Imai S, Yamamizu K, Morita H, Suzuki T, Okada Y, Okano HJ, Yamashita JK, Okano H, Narita M
  • Journal Title: PLoS One Volume: 7(10) Pages: e44368
  • DOI: DOI:10.1371/journal.pone.0044368
  • Peer Reviewed
• [Journal Article] Efficient Derivation of Multipotent Neural Stem/Progenitor Cells from Non-human Primate Embryonic Stem Cells (2012)
  • Author(s): Shimada H, Okada Y, Ibata K, Ebise H, Ota S, Tomioka I, Nomura T, Maeda T, Kohda K, Yuzaki M, Sasaki E, Nakamura M, Okano H
  • Journal Title: PLoS One Volume: 7(11) Pages: e49469
  • DOI: DOI:10.1371/journal.pone.0049469
  • Peer Reviewed
• [Journal Article] Mitochondrial dysfunction associated with increased oxidativestress and α-synuclein accumulation in PARK2 iPSC-derived neurons and postmortem brain tissue (2012)
  • Author(s): Imaizumi Y, Okada Y, Akamatsu W, Koike M, Kuzumaki N, Hayakawa H, Nihira T, Kobayashi T, Ohyama M, Sato S, Takanashi M, Funayama M, Hirayama A, Soga T, Hishiki T, Suematsu M, Yagi T, Ito D, Kosakai A, Hayashi K, Shouji M, Nakanishi A, Suzuki N, Mizuno Y
  • Journal Title: Mol. Brain Volume: 5(1) Pages: 35
  • DOI: DOI:10.1186/1756-6606-5-35
  • Peer Reviewed
• [Journal Article] Establishment of induced pluripotent stem cells from centenarians for neurodegenerative disease research (2012)
  • Author(s): Yagi T, Kosakai A, Ito D, Okada Y, Akamatsu W, Nihei Y, Nabetani A, Ishikawa F, Arai Y, Hirose N, Okano H, Suzuki N
  • Journal Title: PLoS One Volume: 7(7) Pages: e41572
  • DOI: DOI:10.1371/journal.pone.0041572
  • Peer Reviewed
• [Journal Article] RNA-Binding Protein Musashi1 Modulates Glioma Cell Growth through the Post-Transcriptional Regulation of Notch and PI(3) Kinase/Akt Signaling Pathways (2012)
  • Author(s): Muto J, Imai T, Ogawa D, Nishimoto Y, Okada Y, Mabuchi Y, Kawase T, Iwanami A, Mischel PS, Saya H, Yoshida K, Matsuzaki Y, Okano H
  • Journal Title: PLoS One Volume: 7(3) Pages: e33431
  • DOI: DOI:10.1371/journal.pone.0033431
  • Peer Reviewed
• [Journal Article] Effect of κ-opioid receptor agonist on the growth of non-small cell lung cancer (NSCLC) cells (2011)
  • Author(s): Kuzumaki N, Suzuki A, Narita M, Hosoya T, Nagasawa A, Imai S, Yamamizu K, Morita H, Nagase H, Okada Y, Okano HJ, Yamashita JK, Okano H, Suzuki T, Narita M
  • Journal Title: Br J Cancer Volume: 106(6) Pages: 1148-52
  • DOI: DOI:10.1038/bjc.2011.574
  • Peer Reviewed
• [Journal Article] Grafted human induced pluripotent stem cell-derived neurospheres promotes motor functional recovery after spinal cord injury in mice (2011)
  • Author(s): Nori S, Okada Y, Yasuda A, Tsuji O, Takahashi Y, Kobayashi Y, Fujiyoshi K, Koike M, Uchiyama Y, Ikeda E, Toyama Y, Yamanaka S, Nakamura M, Okano H
  • Journal Title: Proc Natl Acad Sci USA Volume: 108(40) Pages: 16825-30
  • DOI: DOI:10.1073/pnas.1108077108
  • Peer Reviewed
• [Journal Article] Modeling familial Alzheimer's disease with induced pluripotent stem cells (2011)
  • Author(s): Yagi T, Ito D, Okada Y, Akamatsu W, Nihei Y, Yoshizaki T, Yamanaka S, Okano H, Suzuki N
  • Journal Title: Hum. Mol. Genet Volume: 20 (23) Pages: 4530-4539
  • DOI: DOI:10.1093/hmg/ddr394
  • Peer Reviewed
• [Journal Article] Musashi 1 cooperates in abnormal cell LINeage protein 28 (Lin28)-mediated Let-7 family microRNA biogenesis in early neural differentiation (2011)
  • Author(s): Kawahara H, Okada Y, Imai T, Iwanami A, Mischel PS, Okano H
  • Journal Title: J Biol Chem Volume: 286(18) Pages: 16121-30
  • DOI: DOI:10.1074/jbc.M110.199166
  • Peer Reviewed
• [Journal Article] Generation of human melanocytes from induced pluripotent stem cells (2011)
  • Author(s): Ohta S, Imaizumi Y, Okada Y, Akamatsu W, Kuwahara R, Ohyama M, Amagai M, Matsuzaki Y, Yamanaka S, Okano H, Kawakami Y
  • Journal Title: Plos One Volume: 6 (1) Pages: e16182
  • DOI: DOI:10.1371/journal.pone.0016182
  • Peer Reviewed
• [Journal Article] Gene targeting and subsequent site-specific transgenesis at the β-actin (ACTB) locus in common marmoset embryonic stem cells (2011)
  • Author(s): Shiozawa S, Kawai K, Okada Y, Tomioka I, Maeda T, Kanda A, Shinohara H, Suemizu H, Okano HJ, Sotomaru Y, Sasaki E, Okano H
  • Journal Title: Stem Cells and Development Volume: 20(9) Pages: 1587-99
  • DOI: DOI:10.1089/scd.2010.0351
  • Peer Reviewed
• [Journal Article] Human Decidua-Derived Mesenchymal Cells are a Promising Source for the Generation and Cell Banking, of Human Induced Pluripotent Stem Cells Cell Medicine
  • Author(s): Shofuda T, Kanematsu D, Fukusumi H, Yamamoto A, Bamba Y, Yoshitatsu S, Suemizu H, Nakamura M, Sugimoto Y, Furue KM, Kohara A, Akamatsu W, Okada Y, Okano H, Yamasaki M, Kanemura Y
  • Journal Title: Part B of Cell Transplantation Volume: (in press)
  • Peer Reviewed
• [Presentation] Incompletely reprogrammed human iPSCs form glioma-like tumors through genomic instability during neural differentiation (2013)
  • Author(s): Okada Y, Miya, F, Koike M, Tomisato S, Tokura T, Ishihara Y, Shimojo D, Hattori C, Kanematsu D, Kanemura Y, Kohda K, Sobue G, Yamanaka S, Yuzaki M, Uchiyama Y, Ikeda E, Tsunoda T, Okano H
  • Organizer: CiRA International Symoisum 2013, Raising the Nex Generation of Stem Cell Research, Kyoto March 2013, CiRA International Symoisum 2013, Raising the Nex Generation of Stem Cell Research
  • Place of Presentation: Kyoto
  • Year and Date: 2013-03-12
• [Presentation] Modeling dysmyelinating neurological disorders using human iPSC-derived mature oligodendrocytes (2013)
  • Author(s): Numasawa Y, Okada Y, Shibata S, Kuzumaki N, Kishi N, Akamatsu W, Amagai M, Osaka H, Inoue K, Takahashi K, Yamanaka S, Kosaki K, Takahashi T, Okano H
  • Organizer: CiRA International Symoisum 2013, Raising the Nex Generation of Stem Cell Research
  • Place of Presentation: Kyoto
  • Year and Date: 2013-03-11
• [Presentation] iPS細胞を用いた神経再生と病態解析 (2013)
  • Author(s): 岡田洋平
  • Organizer: 第4回岐阜脳神経研究会
  • Place of Presentation: 岐阜
  • Year and Date: 2013-02-02
• [Presentation] 不完全にリプログラミングされたヒトiPS細胞由来神経幹細胞は分化誘導に伴うゲノム不安定化によりグリオーマ様腫瘍を形成する (2012)
  • Author(s): 岡田洋平、宮冬樹、小池正人、冨里周太、兼松大介、金村米博、幸田和久、柚崎通介、内山安男、池田栄二、角田達彦、山中伸弥、岡野栄之
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-12
• [Presentation] ヒトiPS細胞由来神経幹細胞の応用とその問題点 (2012)
  • Author(s): 岡田洋平、岡野栄之
  • Organizer: 第24回日本脳循環代謝学会総会
  • Place of Presentation: 広島
  • Year and Date: 2012-11-09
• [Presentation] 多能性幹細胞・神経幹細胞を用いた神経再生と病態解析 (2012)
  • Author(s): 岡田洋平
  • Organizer: 第13回埼玉パーキンソン病治療研究会
  • Place of Presentation: 大宮
  • Year and Date: 2012-11-01
• [Presentation] Evaluation of human iPS cells byneural differentiation and tumorigenicity (2012)
  • Author(s): Okada Y, Miya F, Tomisato S, Kanemura Y, Koike M, Kohda K, Yuzaki M, Uchiyama Y, Tsunoda T, Yamanaka S, Okano H
  • Organizer: ISSCR 10th annual Meeting
  • Place of Presentation: Yokohama
  • Year and Date: 2012-06-15
• [Presentation] Rapid and easy monolayer neural differentiation of human ES cells (2012)
  • Author(s): Ishihara Y, Okada Y, Koakutsu M, Okano H
  • Organizer: ISSCR 10th annual Meeting
  • Place of Presentation: Yokohama
  • Year and Date: 2012-06-14
• [Presentation] Mitochondrial dysfunction and alfa synuclein accumulation in PARK2 IPSC derived neurons and postmortem brain (2012)
  • Author(s): Imaizumi Y, Okada Y, Akamatsu W, Koike M, Hayakawa H, Nihira T, Suematsu M, Suzuki N, Amagai M, Uchiyama Y, Mochizuki H, Hattori N, Okano H
  • Organizer: ISSCR 10th annual Meeting
  • Place of Presentation: Yokohama
  • Year and Date: 2012-06-14
• [Presentation] Exogenous signals oppositely affect adipocyte differentiation and neural sifferentiation of human ES/iPS cells (2012)
  • Author(s): Koakutsu M, Okada Y, Okano H
  • Organizer: ISSCR 10th annual Meeting
  • Place of Presentation: Yokohama
  • Year and Date: 2012-06-14
• [Presentation] Pre-evaluated safe human iPS clone derived neural stem cells promoted functional recovery after spinal cord injury without tumorigenecity in adult common marmosets (2012)
  • Author(s): Kobayashi Y, Okada Y, Iwai H, Nishimura S, Nori S, Konomi T, Fujiyoshi K, Tsuji O, Toyama Y, Yamanaka S, Nakamura M, Okano H
  • Organizer: ISSCR 10th annual Meeting
  • Place of Presentation: Yokohama
  • Year and Date: 2012-06-14
• [Presentation] Transplantation of human iPS cell-derived neurospheres for the treatment of spinal cord injury in NOD-scid mice (2011)
  • Author(s): Nori, S., Okada Y., Yasuda, A., Tsuji, O., Takahashi,1Y., Kobayashi,Y., Fujiyoshi, K., Toyama, Y., Yamanaka, S., Nakamura, M., Okano, H.
  • Organizer: ISSCR 9th annual Meeting
  • Place of Presentation: Toronto, Canada
  • Year and Date: 20110615-18
• [Presentation] Efficient derivation of neural stem cells from common marmoset ES cells and iPS cells. (2011)
  • Author(s): Shimada, H., Okada Y., Tomioka, I., Sasaki, E., Nakamura, M., Okano, H.
  • Organizer: Society for Neuroscience 41th Annual Meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2011-11-15
• [Presentation] 神経分化と造腫瘍性から迫るヒトiPS細胞の品質評価 (2011)
  • Author(s): 岡田洋平、宮冬樹、 金村米博、 砂堀毅彦、 小池正人、幸田和久、柚崎通介、内山安男、 角田達彦、山中伸弥、岡野栄之
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-17
• [Presentation] 神経前駆細胞特異的レポーターヒトES細胞の樹立 (2011)
  • Author(s): 吉田賢司、太田真一、原央子、岡田洋平、岡野栄之
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-15
• [Presentation] Generation and characterization of human ES cells carrying Sox1-reporter gene for neural differentiation. (2011)
  • Author(s): Yoshida, K., Ota, S.-I., Hara, C., Okano, H., Okada Y.
  • Organizer: 8th IBRO World congress of Neuroscience
  • Place of Presentation: Florence, Italy
  • Year and Date: 2011-07-18
• [Presentation] Evaluation of human iPS cells by neural differentiation and tumorigenicity (2011)
  • Author(s): Okada Y, Miya F, Kanemura Y, Koike M, Kohda K, Yuzaki M, Uchiyam Y, Tsunoda T, Yamanaka S, Okano H
  • Organizer: 8th IBRO World congress of Neuroscience
  • Place of Presentation: Florence, Italy
  • Year and Date: 2011-07-16
• [Presentation] iPS細胞・神経幹細胞で神経系を再生する (2011)
  • Author(s): 岡田洋平
  • Organizer: 兵庫県保険医協会第79回総会特別講演
  • Place of Presentation: 神戸
  • Year and Date: 2011-06-19
• [Presentation] 神経分化と造腫瘍性から迫るヒトiPS細胞の品質評価 (2011)
  • Author(s): 岡田洋平、岡野栄之
  • Organizer: 第52回日本神経学会学術大会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-05-20
• [Book] Brain Nerve (2012)
  • Author(s): 海苔聡、辻収彦、岡田洋平、戸山芳昭、岡野栄之、中村雅也
  • Total Pages: 17-27
  • Publisher: 医学書院
• [Book] 臨床神経学 (2011)
  • Author(s): 岡田洋平、岡野栄之
  • Total Pages: 1079-1080
  • Publisher: 日本神経学会
• [Remarks]
  • URL: http://www.careerpath-prj.keio.ac.jp/kanrinmaru/scholar/okada/index.html
• [Patent(Industrial Property Rights)] iPS細胞のクローンの選択方法、及びその選択方法に用いる遺伝子の選択方法 (2012)
  • Inventor(s): 岡田洋平、岡野栄之、角田達彦、宮冬樹
  • Industrial Property Rights Holder: 学校法人慶應義塾
  • Industrial Property Number: PCT/JP2012/054800
  • Filing Date: 2012-02-27
  • Overseas