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2012 Fiscal Year Final Research Report

Optically regulating the synaptic plasticity at the level of single synaptic spine.

Research Project

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Project/Area Number 23650197
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Neurochemistry/Neuropharmacology
Research InstitutionWaseda University

Principal Investigator

NAKAMURA Hideki  早稲田大学, 理工学術院, 助教 (50435666)

Project Period (FY) 2011 – 2012
Keywords神経可塑性
Research Abstract

As a preliminary step toward the establishment of technical methods to manipulate the synaptic transmission by rapamycin-induced protein dimerization paradigm, two plasmid libraries were constructed; PSD-95-based construct library and Rho GTPase family-based library. These libraries will contribute to the development of rapamycin-based methods to study synaptic plasticity in neuronal cells. To evaluate the rapamycin-induced protein dimerization within neurons, methods to monitor the diffusion kinetics of soluble proteins in the cell type were also established.

Research Products

(4 results)

All 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results)

  • [Journal Article] Proteomic analysis of multiple primary cilia reveals a novel mode of ciliary development in mammals2012

    • Author(s)
      Narita K., Kozuka-Hata H., Nonami Y.,Ao-Kondo H.,Suzuki T., Nakamura H., Yamakawa K., Oyama M., Inoue T., Takeda S
    • Journal Title

      Biology Open

      Volume: Vol. 1 No. 8 Pages: 815-825

    • DOI

      doi:10.1242/bio.20121081

    • Peer Reviewed
  • [Journal Article] Cooeprative and stochastic calcium releases from multiple calcium puff sites generate calcium microdomains in intact HeLa cells2012

    • Author(s)
      Nakamura H., Bannai H., Inoue T., Michikawa T., Mikoshiba K
    • Journal Title

      Journal of Biological Chemistry

      Volume: Vol.287 No.29 Pages: 24563-24572

    • DOI

      doi:10.1074/jbc.M111.311399

    • Peer Reviewed
  • [Journal Article] Highly cooperative dependence of sarco/endoplasmic reticulum calcium ATPase SERCA2a pump activity on cytosolic calcium in living cells2011

    • Author(s)
      Satoh K, Matsu-Ura T, Enomoto M, Nakamura H, Michikawa T, Mikoshiba K
    • Journal Title

      Journal of Biological Chemistry

      Volume: Vol 286 No. 23 Pages: 20591-20599

    • DOI

      doi:10.1074/jbc.M110.204685

    • Peer Reviewed
  • [Journal Article] Chemically inducible diffusion trap at cilia reveals molecular sieve-like barrier

    • Author(s)
      Lin Y.C., Niewiadomski P., Lin B., Nakamura H., Phua S.C., Jiao J., Levchenko A., Inoue T., Rohatgi R., Inoue T
    • Journal Title

      Nature Chemical Biology

      Volume: (in press)

    • DOI

      doi:10.1038/nchembio.1252

    • Peer Reviewed

URL: 

Published: 2014-09-25  

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