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2012 Fiscal Year Final Research Report

Optically regulating the synaptic plasticity at the level of single synaptic spine.

Research Project

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Project/Area Number 23650197
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Neurochemistry/Neuropharmacology
Research InstitutionWaseda University

Principal Investigator

NAKAMURA Hideki  早稲田大学, 理工学術院, 助教 (50435666)

Project Period (FY) 2011 – 2012
Keywords神経可塑性
Research Abstract

As a preliminary step toward the establishment of technical methods to manipulate the synaptic transmission by rapamycin-induced protein dimerization paradigm, two plasmid libraries were constructed; PSD-95-based construct library and Rho GTPase family-based library. These libraries will contribute to the development of rapamycin-based methods to study synaptic plasticity in neuronal cells. To evaluate the rapamycin-induced protein dimerization within neurons, methods to monitor the diffusion kinetics of soluble proteins in the cell type were also established.

  • Research Products

    (4 results)

All 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results)

  • [Journal Article] Proteomic analysis of multiple primary cilia reveals a novel mode of ciliary development in mammals2012

    • Author(s)
      Narita K., Kozuka-Hata H., Nonami Y.,Ao-Kondo H.,Suzuki T., Nakamura H., Yamakawa K., Oyama M., Inoue T., Takeda S
    • Journal Title

      Biology Open

      Volume: Vol. 1 No. 8 Pages: 815-825

    • DOI

      doi:10.1242/bio.20121081

    • Peer Reviewed
  • [Journal Article] Cooeprative and stochastic calcium releases from multiple calcium puff sites generate calcium microdomains in intact HeLa cells2012

    • Author(s)
      Nakamura H., Bannai H., Inoue T., Michikawa T., Mikoshiba K
    • Journal Title

      Journal of Biological Chemistry

      Volume: Vol.287 No.29 Pages: 24563-24572

    • DOI

      doi:10.1074/jbc.M111.311399

    • Peer Reviewed
  • [Journal Article] Highly cooperative dependence of sarco/endoplasmic reticulum calcium ATPase SERCA2a pump activity on cytosolic calcium in living cells2011

    • Author(s)
      Satoh K, Matsu-Ura T, Enomoto M, Nakamura H, Michikawa T, Mikoshiba K
    • Journal Title

      Journal of Biological Chemistry

      Volume: Vol 286 No. 23 Pages: 20591-20599

    • DOI

      doi:10.1074/jbc.M110.204685

    • Peer Reviewed
  • [Journal Article] Chemically inducible diffusion trap at cilia reveals molecular sieve-like barrier

    • Author(s)
      Lin Y.C., Niewiadomski P., Lin B., Nakamura H., Phua S.C., Jiao J., Levchenko A., Inoue T., Rohatgi R., Inoue T
    • Journal Title

      Nature Chemical Biology

      Volume: (in press)

    • DOI

      doi:10.1038/nchembio.1252

    • Peer Reviewed

URL: 

Published: 2014-09-25  

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