2012 Fiscal Year Final Research Report
Optically regulating the synaptic plasticity at the level of single synaptic spine.
| Project/Area Number |
23650197
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 神経可塑性 |
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| Research Abstract |
As a preliminary step toward the establishment of technical methods to manipulate the synaptic transmission by rapamycin-induced protein dimerization paradigm, two plasmid libraries were constructed; PSD-95-based construct library and Rho GTPase family-based library. These libraries will contribute to the development of rapamycin-based methods to study synaptic plasticity in neuronal cells. To evaluate the rapamycin-induced protein dimerization within neurons, methods to monitor the diffusion kinetics of soluble proteins in the cell type were also established.
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