2012 Fiscal Year Final Research Report
Study on the role of extracellular deubiquitinating enzymes
| Project/Area Number |
23650199
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | National Center of Neurology and Psychiatry |
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Principal Investigator |
WADA Keiji 独立行政法人国立精神・神経医療研究センター, 神経研究所疾病研究第四部, 部長 (70250222)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 蛋白質 / 脳神経疾患 / 神経科学 / 生理活性 / 細胞 / 組織 |
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| Research Abstract |
We aimed to elucidate the biological role of extracellular UCH-L1. To achieve this purpose, we used cultured cells and studied whether the protein was secreted or not into the media. We observed that UCH-L1 was secreted into the media via a unconventional pathway. Interestingly, exosome contained UCH-L1. I93M UCH-L1 mutant was less secreted compared with wild type of UCH-L1. Then we studied a possible interaction of UCH-L1 with other disease-related proteins in the secretory event. We found that the secretion of UCH-L1 was modified by the presence of mutated alpha-synuclein and huntingtin. These results suggest that secretion of UCH-L1 into extracellular space might be related to the pathophysiological conditions of neurodegenerative disorders.
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Research Products
(10 results)
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[Journal Article] Ubiquitin C-terminal hydrolase L1 (UCH-L1) act as a novel potentiator of cyclin-dependent kinases to enhance cell proliferation, independent of its hydrolase activity2013
Author(s)
Kabuta, T., Mitsui, T., Takahashi, M., Fujiwara, Y., Kabuta, C., Konya, C., Tsuchiya, Y., Hatanaka, Y., Uchida, K., Hohjoh, H., Wada, K
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Journal Title
J. Biol. Chem
Volume: 288
Pages: 12615-12626
DOI
Peer Reviewed
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[Journal Article] Sulfatide accumulation in the dystrophic terminals of gracile axonal dystrophy mice: lipid analysis using matrix-assisted laser desorption/ionization imaging mass spectrometry2013
Author(s)
Onishi, K., Tasumi, Y., Wada, K., Yang, H.J., Sugiura, Y., Setou, M., Yoshikawa, H
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Journal Title
Med. Mol. Morphol
Volume: (Epub ahead of print)
Peer Reviewed
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[Journal Article] Control of BACE1 and APP processing by ubiquitin carboxyl-terminal hydrolase L1 in Alzheimer's Disease Pathogenesis2012
Author(s)
Zhang, M., Deng, Y., Luo, Y., Zhang, S., Zou, H., Cai, F., Wada, K., Song, W
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Journal Title
J. Neurochem
Volume: 120
Pages: 1129-1138
DOI
Peer Reviewed
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[Journal Article] Selective Neuroprotective Effects of the S18Y Polymorphic Variant of UCH-L1 in the dopaminergic system2012
Author(s)
Xilouri, M., Kyratzi, E., Pitychoutis, P.M., Papadopoulou-Daifoti, Z., Celine Perier, C., Vila, M., Maniati, M., Ayse Ulusoy, A., Kirik, D., David S. Park, D.S., Wada, K., Stefanis, L
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Journal Title
Hum. Mol. Genet
Volume: 21
Pages: 874-879
DOI
Peer Reviewed
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[Journal Article] Parkinson's disease-associated mutations in alpha-synuclein and UCH-L1 inhibit the unconventional secretion of UCH-L12011
Author(s)
Konya, C., Hatanaka, Y., Fujiwara, Y., Uchida, K., Nagai, Y., Wada, K., Kabuta, T
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Journal Title
Neurochem Int
Volume: 59
Pages: 251-258
DOI
Peer Reviewed
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