• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2012 Fiscal Year Final Research Report

Targeted gene expression in single neurons by opto-poration in vivo

Research Project

  • PDF
Project/Area Number 23650204
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Neurophysiology and muscle physiology
Research InstitutionThe University of Tokyo

Principal Investigator

KANO Masanobu  東京大学, 大学院・医学系研究科, 教授 (40185963)

Project Period (FY) 2011 – 2012
Keywords神経科学 / 2 光子イメージング / 遺伝子発現
Research Abstract

We develop a novel method to introduce exogenous genes in individual neurons of interest in the intact brain. By using a mode-locked Ti:Sapphire laser, the neuronal membrane can be perforated to deliver small molecules including fluorescent dye and DNA plasmid. We have confirmed that fluorescent dye molecules could be reliably introduced into individual neurons in the intact mouse brains. After optimization of the technique, it is expected to be able to deliver DNA plasmid and targeted exogenous gene expression in vivo.

  • Research Products

    (47 results)

All 2013 2012 2011 Other

All Journal Article (16 results) (of which Peer Reviewed: 12 results) Presentation (31 results)

  • [Journal Article] The endocannabinoid2-arachidonoylglycerol negatively regulates habituation by suppressing excitatory recurrent network activity and reducing long-term potentiation in the dentate gyrus2013

    • Author(s)
      Sugaya, Y., Cagniard, B., Yamazaki, M., Sakimura, K. & Kano, M.
    • Journal Title

      J. Neurosci

      Volume: 33 Pages: 3588-3601

    • Peer Reviewed
  • [Journal Article] Spatiotemporal Dynamics of Functional Clusters of Neurons in the Mouse Motor Cortex during a Voluntary Movement2013

    • Author(s)
      Hira, R., Ohkubo, F., Ozawa, K., Isomura, Y., Kitamura, K., Kano, M., Kasai, H. & Matsuzaki, M.
    • Journal Title

      J. Neurosci

      Volume: 33 Pages: 1377-1390

    • Peer Reviewed
  • [Journal Article] Dendritic calciumsignaling in cerebellar Purkinje cell2012

    • Author(s)
      Kitamura, K. & Kano, M.
    • Journal Title

      Neural Networks published online

    • URL

      http://www.sciencedirect.com/science/article/pii/S0893608012002110

    • Peer Reviewed
  • [Journal Article] GABAergic inhibition regulates developmental synapse elimination in the cerebellum2012

    • Author(s)
      Nakayama, H., Miyazaki, T., Kitamura, K., Hashimoto, K., Yanagawa, Y.,Obata, K., Sakimura, K., Watanabe, M.& Kano, M.
    • Journal Title

      Neuron

      Volume: 74 Pages: 384-396

    • Peer Reviewed
  • [Journal Article] Organotypic coculture preparation for the study of developmental synapseelimination in mammalian brain2012

    • Author(s)
      Uesaka, N., Mikuni, T., Hashimoto, K., Hirai, H., Sakimura, K. & Kano, M.
    • Journal Title

      J. Neurosci

      Volume: 32 Pages: 11688-11699

    • Peer Reviewed
  • [Journal Article] Synapsetype-independent degradation of the endocannabinoid2-arachidonoylglycerol after retrograde synaptic suppression2012

    • Author(s)
      Tanimura, A., Uchigashima, M., Yamazaki, M., Uesaka, N., Mikuni, T., Abe, M., Hashimoto, K., Watanabe, M., Sakimura, K. & Kano, M.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 109 Pages: 12195-12200

    • Peer Reviewed
  • [Journal Article] Synaptogenesis and synaptic elimination2012

    • Author(s)
      Kano, M. & Watanabe, M.
    • Journal Title

      Handbook of Cerebellum and Cerebellar Disorders

      Volume: (in press)

  • [Journal Article] Locally synchronized synaptic inputs2012

    • Author(s)
      Takahashi, N., Kitamura, K., Matsuo, N., Mayford, M., Kano, M., Matsuki, N.& Ikegaya, Y.
    • Journal Title

      Science

      Volume: 335 Pages: 353-356

    • Peer Reviewed
  • [Journal Article] 小脳プルキンエ細胞樹状突起活動のin vivoイメージング2012

    • Author(s)
      喜多村和郎,橋本浩一,狩野方伸
    • Journal Title

      生体の科学

      Volume: 63 Pages: 26-33

  • [Journal Article] CaV2.1 in cerebellar Purkinje cellsregulates competitive excitatory synaptic wiring, cell survival, and cerebellar biochemical compartmentalization2012

    • Author(s)
      Miyazaki, T., Yamasaki, M., Hashimoto, K., Yamazaki, M., Abe, M., Usui, H., Kano, M., Sakimura, K. & Watanabe,M.
    • Journal Title

      J. Neurosci

      Volume: 32 Pages: 1311-1328

    • Peer Reviewed
  • [Journal Article] Climbing fiber synapse elimination in cerebellar Purkinje cells2011

    • Author(s)
      Watanabe, M. & Kano, M.
    • Journal Title

      Eur. J. Neurosci

      Volume: 34 Pages: 1697-1710

  • [Journal Article] Developmental switching ofperisomatic innervation from climbing fibers to basket cell fibers in cerebellarPurkinje cells2011

    • Author(s)
      Ichikawa, R., Yamasaki, M., Miyazaki, T., Konno, K., Hashimoto, K., Tatsumi, H., Inoue, Y., Kano, M. & Watanabe, M.
    • Journal Title

      J. Neurosci

      Volume: 31 Pages: 16916-16927

    • Peer Reviewed
  • [Journal Article] Postsynaptic P/Q-type Ca2+ channel in Purkinje cell mediates synaptic competition and elimination in developing cerebellum2011

    • Author(s)
      Hashimoto, K., Tsujita, M., Miyazaki, T., Kitamura, K., Yamazaki, M., Shin, H.-S., Watanabe, M., Sakimura, K. & Kano, M.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 108 Pages: 2447-2457

    • Peer Reviewed
  • [Journal Article] In vivo two-photon uncaging of glutamate revealing the structure-function relationships of dendritic spines in the neocortex of adult mice2011

    • Author(s)
      Noguchi, J., Nagaoka, A., Watanabe, S., Ellis-Davies, G.C., Kitamura, K., Kano, M., Matsuzaki, M. & Kasai, H.
    • Journal Title

      J.Physiol

      Volume: 589 Pages: 2447-2457

    • Peer Reviewed
  • [Journal Article] A role for myosin Va in cerebellar plasticity and motor learning: a possible mechanism underlying neurological disorder in myosin Va disease2011

    • Author(s)
      Miyata, M., Kishimoto, Y., Tanaka, M., Hashimoto, K., Hirashima, N., Murata, Y., Kano, M. & Takagishi, Y.
    • Journal Title

      J. Neurosci

      Volume: 31 Pages: 6067-6078

  • [Journal Article] Calcium-dependent regulation of climbing fiber synapse elimination during postnatal cerebellardevelopment

    • Author(s)
      Kano, M., Nakayama, H., Hashimoto, K., Kitamura, K., Sakimura, K. & Watanabe, M.
    • Journal Title

      J. Physiol

      Volume: (in press)

    • Peer Reviewed
  • [Presentation] マウスバレル皮質における感覚シナプス入力の可視化2013

    • Author(s)
      喜多村和郎、狩野方伸
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Year and Date
      20130327-29
  • [Presentation] マウス小脳における登上線維応答の同期性とAldolaseC発現パターンとの関連2013

    • Author(s)
      堤新一郎、多田真弓、崎村建司、喜多村和郎、狩野方伸
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Year and Date
      20130327-29
  • [Presentation] Climbing fiber network refinementand desynchronization of Purkinje cellpopulation activity during postnatal cerebellar development2013

    • Author(s)
      Jean-Marc Good, Kazuo Kitamura, Kenji Sakimura, Masanobu Kano
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Year and Date
      20130327-29
  • [Presentation] 組織培養法と細胞種特異的遺伝子導入法を用いたシナプス刈り込みのメカニズム2013

    • Author(s)
      上阪直史、三國貴康、平井宏和、狩野方伸
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Year and Date
      20130327-29
  • [Presentation] 内因性カンナビノイド2-アラキドノイルグリセロールは歯状回の興奮性シナプス伝達を抑制し発作を減弱する2013

    • Author(s)
      菅谷佑樹、山崎真弥、崎村建司、狩野方伸
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Year and Date
      20130327-29
  • [Presentation] 小脳登上線維-プルキンエ細胞シナプスの発達において競合する登上線維間の相対的な強度の差と登上線維の絶対的な強度がそれぞれ異なる段階に寄与している2013

    • Author(s)
      川田慎也、橋本浩一、山崎真弥、宮崎太輔、山崎美和子、三國貴康、渡辺雅彦、崎村建司、狩野方伸
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Year and Date
      20130327-29
  • [Presentation] Activity-dependent Refinement of Climbing Fiber to Purkinje Cell Synapses during Postnatal Cerebellar Development. International Symposium on "Sensory Systems and Neural Circuits"2013

    • Author(s)
      Kano, M.
    • Organizer
      Celebrating the 22nd Anniversary of Odorant- Receptor Gene Discoveries
    • Place of Presentation
      東京
    • Year and Date
      2013-02-11
  • [Presentation] Synapse type-independent degradation of 2-arachidonoylglycerol after retrograde synaptic suppression in the cerebellum2012

    • Author(s)
      A. TANIMURA, M. UCHIGASHIMA, M. YAMAZAKI, N. UESAKA, T. MIKUNI, K. HASHIMOTO, M. WATANABE, K. SAKIMURA, M. KANO.
    • Organizer
      Neuroscience 2012
    • Place of Presentation
      New Orleans, USA.
    • Year and Date
      20121013-17
  • [Presentation] Possible involvement of theCa2+-dependent gene Arc in activity-dependent synapse elimination in the cerebellum2012

    • Author(s)
      T. MIKUNI, N. UESAKA, H. OKUNO, H. HIRAI, H. BITO, M. KANO.
    • Organizer
      Neuroscience 2012
    • Place of Presentation
      New Orleans, USA.
    • Year and Date
      20121013-17
  • [Presentation] Visualization of an active ensemble of cortical neurons in vivo using a novel activity-dependent promoter E-SARE2012

    • Author(s)
      T. KAWASHIMA, K. KITAMURA, K. OHKI, K. SUZUKI, M. NONAKA, S. KAMIJO, S. TAKEMOTO-KIMURA, M. KANO, H. OKUNO, H. BITO.
    • Organizer
      Neuroscience 2012
    • Place of Presentation
      New Orleans, USA.
    • Year and Date
      20121013-17
  • [Presentation] Electrical contribution of postsynaptic T-type calcium channels to parallelfiber-Purkinje cell synaptic transmission2012

    • Author(s)
      R. LY, V. UEBELE, J. RENGER, H.PROSSER, A. RANDALL, M. KANO, K. SAKIMURA, B. BARBOUR, P. ISOPE, A. FELTZ.
    • Organizer
      Neuroscience 2012
    • Place of Presentation
      New Orleans, USA.
    • Year and Date
      20121013-17
  • [Presentation] Impairment ofreward-based adaptive choice of actions by local injection of cannabinoid CB1 receptor antagonistinto the striatum of behaving monkeys2012

    • Author(s)
      Y. UEDA, S. R. OKAZAKI, K.YAMANAKA, K. ENOMOTO, M. KANO, M. KIMURA.
    • Organizer
      Neuroscience 2012
    • Place of Presentation
      New Orleans, USA.
    • Year and Date
      20121013-17
  • [Presentation] Postnatal climbing fiber synapse remodeling desynchronizes population activity of Purkinje cells in developing cerebellum2012

    • Author(s)
      Jean- Marc Good, Kazuo Kitamura, Kenji Sakimura, Masanobu Kano.
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Year and Date
      20120918-21
  • [Presentation] 新規活動依存的プロモーターE-SAREを用いた、大脳皮質における機能的回路の同定2012

    • Author(s)
      川島尚之、喜多村和郎、大木研一、鈴木敢三、野中美応、上條諭志、竹本さやか、狩野方伸、奥野浩行、尾藤晴彦
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Year and Date
      20120918-21
  • [Presentation] 発達期マウス網膜-外側膝状体シナプス除去における代謝型グルタミン酸受容体1型の役割2012

    • Author(s)
      鳴島円、内ヶ島基政、橋本浩一、饗場篤、渡辺雅彦、宮田麻理子、狩野方伸
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Year and Date
      20120918-21
  • [Presentation] 発達期小脳におけるシナプス除去カルシウム依存性調節2012

    • Author(s)
      狩野方伸
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋
    • Year and Date
      20120918-21
  • [Presentation] Heterogeneous organization of individual synaptic inputs in the mouse barrel cortex2012

    • Author(s)
      Kazuo Kitamura, Yuji Ikegaya, Masanobu Kano.
    • Organizer
      FENS Forum 2012
    • Place of Presentation
      Barcelona, Spain.
    • Year and Date
      20120714-18
  • [Presentation] Spatial And Temporal Structure Of Cortical Microcircuit Activity For Generating Voluntary Movement2012

    • Author(s)
      Hira R., Ohkubo F., Ozawa K., Isomura Y., Kitamura K., Kano M., Kasai H. & Matsuzaki M.
    • Organizer
      FENS Forum 2012
    • Place of Presentation
      Barcelona, Spain.
    • Year and Date
      20120714-18
  • [Presentation] Calcium-dependent regulation of climbing fiber synapse elimination during postnatal cerebellar development2012

    • Author(s)
      Kano, M.
    • Organizer
      Satellite symposium of SFN 2012 meeting
    • Place of Presentation
      New Orleans, USA.
    • Year and Date
      2012-10-12
  • [Presentation] Retrograde suppression of synaptic transmission by the endocannabinoid 2-arachidonoylglycerol2012

    • Author(s)
      Kano, M.
    • Organizer
      Cell Symposia
    • Place of Presentation
      New Orleans, USA.
    • Year and Date
      2012-10-11
  • [Presentation] Control of synaptic function by endogenous cannabinoid2012

    • Author(s)
      Kano, M.
    • Organizer
      Plenary Lecture at the 8th FENS Forum of European Neuroscience
    • Place of Presentation
      Barcelona, Spain.
    • Year and Date
      2012-07-16
  • [Presentation] Refinement of Climbing Fiber to Purkinje Cell Synapses during Postnatal Cerebellar Development2012

    • Author(s)
      Kano, M.
    • Organizer
      1st International Syposium / 59th NIBB Conference
    • Place of Presentation
      岡崎
    • Year and Date
      2012-03-11
  • [Presentation] Postsynaptic P/Q typevoltage-dependent Ca2+ channel is involved in refinement of cerebellar climbing fiber to Purkinje cell synapses in early postnatal development2011

    • Author(s)
      Hashimoto, K., Tsujita, M., Miyazaki,T., Kitamura, K., Yamazaki M., Shin,H-S, Watanabe M., Sakimura K., Kano, M.
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      20110914-17
  • [Presentation] Roles of Purkinje cell activity in climbing fiber synapse elimination in an organotypic coculture preparation of the cerebellum and medullao blongata2011

    • Author(s)
      Mikuni, T., Uesaka, M., Kano, M.
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      20110914-17
  • [Presentation] Enhanced synchrony of climbingfiber-induced Ca2+ signaling in cerebellar Purkinje cells caused by elevated synchronous activity of inferior olivary neurons in GluD2 (GluRδ2) knockout mouse2011

    • Author(s)
      Miki Hashizume, Kazuo, Kitamura, Kenji Sakimura and Masanobu Kano.
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      20110914-17
  • [Presentation] Desynchronization of cerebellar Purkinje cell population activity during postnatal development2011

    • Author(s)
      Good, J.K., Kitamura, K., Kano, M.
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      20110914-17
  • [Presentation] Requirement oftype 1 metabotropic glutamate receptor for experience-dependent pruning of retinogeniculate synapses2011

    • Author(s)
      Narushima, M., Uchigashima, M., Hashimoto, K., Aiba, A., Watanabe, M., Miyata, M., Kano, M.
    • Organizer
      8th IBRO World Congress
    • Place of Presentation
      Florence, Itary.
    • Year and Date
      20110714-18
  • [Presentation] Activity-dependent refinement of climbing fiber to Purkinje cell synapses during postnatal cerebellar development2011

    • Author(s)
      Kano, M., Hashimoto, K., Kawamura, Y., Nakayama, H., Kitamura, K.,Sakimura K.
    • Organizer
      COLD SPRING HARBOR ASIA CONFERENCE: "Assembly, Plasticity, Dysfunction and Repair of NeuralCircuits"
    • Place of Presentation
      蘇州、中国
    • Year and Date
      2011-10-19
  • [Presentation] Activity-dependent maturation of climbing fiber to Purkinje cell synapses during postnatal cerebellar development2011

    • Author(s)
      Kano, M.
    • Organizer
      4th International Symposium of the Society for Research on the Cerebellum
    • Place of Presentation
      東京
    • Year and Date
      2011-09-18
  • [Presentation] Activity-dependent synapse elimination during postnatal development2011

    • Author(s)
      Kano, M.
    • Organizer
      Gordon Research Conference on Cerebellum
    • Place of Presentation
      New London, USA
    • Year and Date
      2011-08-24
  • [Presentation] Activity-dependentrefinement of climbing fiber to Purkinje cell synapses duringpostnatal development2011

    • Author(s)
      Kano, M.
    • Organizer
      Symposium on Organization and Function of Neuronal Circuits in Movement Control
    • Place of Presentation
      Trolleholm Castle, Sweden
    • Year and Date
      2011-05-28

URL: 

Published: 2014-08-29   Modified: 2014-10-02  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi