2012 Fiscal Year Final Research Report
Promotion of the 4Rs in animal experiments by the development of a production process for polyclonal antibodies using a goldfish
| Project/Area Number |
23650227
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Tohoku University |
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Principal Investigator |
ASANO Ryutaro 東北大学, 大学院・工学研究科, 准教授 (80323103)
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| Co-Investigator(Kenkyū-buntansha) |
UMETSU Mitsuo 東北大学, 大学院・工学研究科, 准教授 (70333846)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 実験動物福祉 / 免疫学 / 生体分子 / 蛋白質 / ポリクローナル抗体 / キンギョ / リ ンパ液 / 魚類抗体 |
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| Research Abstract |
In this study, we tried to the development of a production process for polyclonal antibodies using a particular kind of goldfish, in which lymph can be sustainably and easily collected from its enlarged corneas containing antibodies. We successfully purified polyclonal antibodies in lymph extracted from goldfish and established the detection system for them based on ELISA. Further, we confirmed it by dot blotting that specific antibodies in lymph can be produced by examining adjuvants at the immunization.
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[Journal Article] Construction and humanization of a functional bispecific EGFR × CD16 diabody using a refolding system2012
Author(s)
Asano R., Nakayama M., Kawaguchi H., Kubota T., Nakanishi T., Umetsu M., Hayashi H., Katayose Y., Unno M., KudoT., Kumagai I.
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Journal Title
FEBS J
Volume: 279(2)
Pages: 223-233
DOI
Peer Reviewed
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[Journal Article] Invitro and in vivo antitumor effects of recombinant bispecific antibodies based on humanized anti-EGFR antibody2011
Author(s)
Watanabe Y., Asano R., Arai K., Shimomura I., Ogata H., Kawaguchi H., Hayashi H., Ohtsuka H., Yoshida H., Katayose Y., Egawa S., Nakanishi T., Umetsu M., Yasui H., Ishida T., Imai K., Kudo T., Unno M., Kumagai I.
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Journal Title
Oncol. Rep
Volume: 26(4)
Pages: 949-955
DOI
Peer Reviewed
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