2012 Fiscal Year Final Research Report
Development of rat ES cells carrying rat Nanog reporter gene
| Project/Area Number |
23650229
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | ラット / ES細胞 |
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| Research Abstract |
To investigate the establishment of germline-competent rat ES cells, BAC transgenic rats carrying Venus gene and puromycin-resistant gene under the control of rat Nanog gene were generated successfully. Furthermore, we showed that theBAC DNA was inserted into a single endogenous Nanog allele in rat ES cells, suggesting the usefulness of BAC homologous recombination in rat ES cells.
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[Journal Article] Novel ROSA26 Cre-reporter knock-in C57BL/6N mice exhibiting green emission before and red emission after Cre-mediated recombination2013
Author(s)
Hasegawa Y, Daitoku Y, Sekiguchi K, Tanimoto Y, Iijima S, Mizuno S, Ema M, Miwa Y, Mekada K, Yoshiki A, Takahashi S, Sugiyama F, Yagami K
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Journal Title
Exp Anim
Volume: 62(in press)
Peer Reviewed
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