2013 Fiscal Year Final Research Report
Identification of novel homing molecules of hematopoietic stem cells and development of efficient bone marrow transfer methods by modifying carbohydrates
| Project/Area Number |
23650232
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Kanazawa University |
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Principal Investigator |
ASANO Masahide 金沢大学, 学際科学実験センター, 教授 (50251450)
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| Co-Investigator(Kenkyū-buntansha) |
HASHIMOTO Noriyoshi 金沢大学, 金沢大学, 准教授 (50242524)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 糖鎖 / 骨髄幹細胞 / 骨髄移植 / ホーミング |
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| Research Abstract |
We have found that beta4GalT-I-deficient bone marrow (BM) cells cannot engraft in the BM of X-ray radiated wild-type mice. Not only beta4GalT-I-deficient BM cells cannot establish hematopoietic system of recipient mice, but also their engraftment into the BM shortly after BM transfer was markedly reduced, indicating that homing of hematopoietic stem cells (HSC) into the BM was impaired. We next investigated mice having a point mutation in the GNE gene, that encodes a key enzyme for sialic acids biosynthesis. Homing efficiency of BM cells from GNE point-mutant mice was reduced to half that of wild-type mice, although the reduction was not prominent as in beta4GalT-I-deficient mice. These results suggest that sialic acids at the terminus of carbohydrate chains also play a role in HSC homing.
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[Journal Article] Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach2012
Author(s)
Sugahara, D., Kaji, H., Sugihara, K., Asano, M., and Narimatsu, H
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Journal Title
Scientif ic Reports
Volume: 2
Pages: 680
DOI
Peer Reviewed
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[Journal Article] Altered oligosaccharide structures reduce colitis induction in mice defective in β-1, 4-galactosyltransferase2012
Author(s)
Shinzaki, S., Iijima, H., Fujii, H., Kuroki, E., Tatsunaka, N., Inoue, T., Nakajima, S., Egawa, S., Kanto, T., Tsujii, M., Morii, E., Takeishi, S., Asano, M., Takehara, T., Hayashi, N. and Miyoshi, E
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Journal Title
Gastroenterology
Volume: 142
Pages: 1172-1182
DOI
Peer Reviewed
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[Journal Article] Glycoprotein hyposialylation gives rise to a nephrotic-like syndrome that is prevented by sialic acid administration in GNE V572L point-mutant mice2012
Author(s)
Ito, M., Sugihara, K., Asaka, T ., T oyama, T ., Y oshihara, T ., Furuichi, K., Wada, T . and Asano, M
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Journal Title
PLoS ONE
Volume: 7
Pages: e29873
DOI
Peer Reviewed
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[Journal Article] Specific enzyme complex of β-1, 4-galactosyltransferase-II and GlcAT-P facilitates biosynthesis of N-linked HNK-1 carbohydrate2011
Author(s)
Kouno, T., Kizuka, Y., Nakagawa, N., Yoshihara, T., Asano, M. and Oka, S
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Journal Title
J . Biol. Chem
Volume: 286
Pages: 31337-31346
DOI
Peer Reviewed
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[Presentation] Hyposialylation of glycoproteins in the UDP-N-acetylglucosamine-2-epimerase/N -acetylmannosamine kinase (GNE) point mutant mice gives rise to a renal impairment that is prevented by sialic acid administration2011
Author(s)
Ito, M., Asaka, T ., Sugihara, K., Y oshihara, T ., Wada, T . and Asano, M
Organizer
Kidney Week 2011
Place of Presentation
Pennsylvania Convention Center , Philadelphia, USA
Year and Date
2011-11-11
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