2013 Fiscal Year Final Research Report
Establishment of transgenic musk shrews (Suncus murinus)
| Project/Area Number |
23650233
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | The University of Tokyo (2012-2013)
Nagoya University (2011) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HIRABAYASHI Masumi 生理学研究所, 行動・代謝分子解析センター, 准教授 (20353435)
INOUE Naoko 名古屋大学, 大学院・生命農学研究科, 助教 (90377789)
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| Project Period (FY) |
2011 – 2013
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| Keywords | スンクス / 遺伝子改変動物 / GnRH2 |
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| Research Abstract |
In the present study, we have established embryo manipulation techniques, such as fertilized egg collection, induction of pseudopregnancy and embryo transfer in the musk shrew. Furthermore, we successfully produced a pup from transgenic embryos in the musk shrew. So far, we have not yet produced the transgenic musk shrew expressing Venus reporter gene, but those methods established in the present study lead to generate transgenic musk shrews in the near future. In addition, we have identified promoter sequence of musk shrew GnRH2 gene, which is deficient in other rodent animal models, so that we generate GnRH2-Venus transgenic musk shrew to uncover the physiological role of GnRH2 in primates including humans.
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[Journal Article] Epigenetic regulation of Kiss1 gene expression mediating estrogen-positive feedback action in the mouse brain2012
Author(s)
Tomikawa J, Uenoyama Y, Ozawa M, Fukanuma T, Takase K, Goto T, Abe H, Ieda N, Minabe S, Deura C, Inoue N, Sanbo M, Tomita K, Hirabayashi M, Tanaka S, Imamura T, Okamura H, Maeda K, Tsukamura H
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Journal Title
Proc Natl Acad Sci USA
Volume: 109
Pages: E1294-1301
Peer Reviewed
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[Journal Article] Kisspeptin neurons mediate reflex ovulation in the musk shrew (Suncus murinus)2011
Author(s)
Inoue N, Sasagawa K, Ikai K, Sasaki Y, Tomikawa J, Oishi S, Fujii N, Uenoyama Y, Ohmori Y, Yamamoto N, Hondo E, Maeda K, Tsukamura H
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Journal Title
Proc Natl Acad Sci U S A
Volume: 108
Pages: 17527-17532
Peer Reviewed
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