Genetic analysis of tameness using Japanese fancy strain JF1

2013 Fiscal Year Final Research Report

• Project/Area Number: 23650243
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: National Institute of Genetics
• Principal Investigator: KOIDE TSUYOSHI 国立遺伝学研究所, 系統生物研究センター, 准教授 (20221955)
• Co-Investigator(Renkei-kenkyūsha): TAKAHASHI Aki 国立遺伝学研究所, 系統生物研究センター, 助教 (30581764)
• Project Period (FY): 2011 – 2013
• Keywords: 育種遺伝

Research Abstract

Japanese fancy mouse strain JF1 shows black and white spots on their coat and exhibits highly obedient phenotype. In contrast, Japanese wild mouse derived strain MSM still exhibits wildness such as higher anxiety as well as high level of aggressive biting towards humans.
It has been reported that the spotting is caused by a mutation in Ednrb gene. Reduced expression of Ednrb gene resulted in aberrant growth/differentiation of melanocyte cells.
In order to examine a role of Ednrb on behaviors, we backcrossed the Ednrb mutation on MSM genetic background, and found that the mouse shows reduced anxiety-like behavior as well as lower level of aggression. The results showed that Ednrb gene has a role of regulating tameness in mice.

Research Products

• [Journal Article] Control of intermale aggression by the medial prefrontal cortex activation in the mouse (2014)
  • Author(s): Takahashi A, Nagayasu K, Nishitani N, Kaneko S, Koide T
  • Journal Title: PLoS ONE Volume: 9(4) Pages: e94657
  • DOI: 10.1371/journal.pone.0094657
  • Peer Reviewed
• [Journal Article] A male-specific QTL for social interaction behavior in mice mapped with automated pattern detection by a hidden Markov model incorporated into newly developed freeware (2014)
  • Author(s): Arakawa T, Tanave A, Ikeuchi S, Takahashi A, Kakihara S, Kimura S, Sugimoto H, Asada N, Shiroishi T, Tomihara K, Tsuchiya T, Koide T
  • Journal Title: Journal of Neuroscience Methods Available online Pages: 21
  • DOI: 10.1016/j.jneumeth.2014.04.012
  • Peer Reviewed
• [Journal Article] Segregation of a QTL cluster for home-cage activity using a new mapping method based on regression analysis of congenic mouse strains (2014)
  • Author(s): Kato S, Ishii A, Nishi A, Kuriki S, Koide T
  • Journal Title: Heredity Advance online publication Pages: 30
  • DOI: 10.1038/hdy.2014.42
  • Peer Reviewed
• [Journal Article] Enhanced prepulse inhibition and low sensitivity to a dopamine agonist in Hesr1-knockout mice (2014)
  • Author(s): Kanno K., Kokubo H., Takahashi A., Koide T., Ishiura S
  • Journal Title: Journal of Neuroscience Research Volume: 92 Pages: 287-297
  • DOI: 10.1002/jnr.23291
  • Peer Reviewed
• [Journal Article] Selection for reluctance to avoid humans during the domestication of mice (2013)
  • Author(s): Goto T., Tanave A., Moriwaki K., Shiroishi T., Koide T
  • Journal Title: Genes, Brain, and Behavior Volume: 12 Pages: 760-770
  • DOI: 10.1111/gbb.12088
  • Peer Reviewed
• [Journal Article] Identification of both copy number variation-type and constant-type core elements in a large segmental duplication region of the mouse genome (2013)
  • Author(s): Umemori J., Mori A., Ichiyanagi K., Uno T., Koide T
  • Journal Title: BMC Genomics Volume: 14 Pages: 455
  • DOI: 10.1186/1471-2164-14-455
  • Peer Reviewed
• [Journal Article] Genomic mixing to elucidate the genetic system of complex traits (2012)
  • Author(s): Koide T., Goto, T., Takano-Shimizu, T
  • Journal Title: Experimental Animals Volume: 61 Pages: 503-509
  • DOI: 10.1538/expanim.61.503
  • Peer Reviewed
• [Journal Article] Behavioral characterization of escalated aggression induced by GABA(B) receptor activation in the dorsal raphe nucleus (2012)
  • Author(s): Takahashi A., Schilit A.N., Kim J., Debold J.F., Koide T., Miczek K.A
  • Journal Title: Psychopharmacology Volume: 224 Pages: 155-166
  • DOI: 10.1007/s00213-012-2654-8
  • Peer Reviewed
• [Journal Article] A role for strain differences in waveforms of ultrasonic vocalizations during male- female interaction (2011)
  • Author(s): Sugimoto H., Okabe S., Kato M., Koshida N., Shiroishi T., Mogi K., Kikusui T., Koide T
  • Journal Title: PLoS ONE Volume: 6 Pages: e22093
  • DOI: 10.1371/journal.pone.0022093
  • Peer Reviewed
• [Journal Article] B6-MSM consomic mouse strains reveal multiple loci for genetic variation in sucrose octaacetate aversion (2011)
  • Author(s): Ishii A., Koide T., Takahashi A., Shiroishi T., Hettinger T.P., Frank M.E., Savoy L.D., Formaker B.K., Yertutanol S., Lionikas A. and Blizard D.A
  • Journal Title: Behavior Genetics Volume: 41 Pages: 716-723
  • DOI: 10.1007/s10519-011-9464-3
  • Peer Reviewed
• [Journal Article] A new twist on behavioral genetics by incorporating wild-derived mouse strains (2011)
  • Author(s): Koide T., Ikeda K., Ogasawara M., Shiroishi T., Moriwaki K., and Takahashi A
  • Journal Title: Experimental Animals Volume: 60 Pages: 347-354
  • DOI: 10.1538/expanim.60.347
  • Peer Reviewed
• [Journal Article] Genetic mapping of escalated aggression in wild-derived mouse strain MSM/Ms : association with serotonin-related genes
  • Author(s): Takahashi A, Shiroishi T, Koide T
  • Journal Title: Frontiers in Neuroscience Volume: (in press)
  • Peer Reviewed
• [Presentation] Genetic mapping and molecular analysis of behavioral response to stress in wild-derived mouse strain (2013)
  • Author(s): Tanave, A., Takahashi, A., Toshihiko, S., Koide, T
  • Organizer: Neuro 2013
  • Place of Presentation: Kyoto
  • Year and Date: 20130620-23
• [Presentation] Interacting molecules of the cell adhesion molecule Caspr3 expressed in the basal ganglia (2013)
  • Author(s): Hirata, H., Juzoh, U., Koide, T., Watanabe, K., Shimoda, Y
  • Organizer: Neuro 2013
  • Place of Presentation: Kyoto
  • Year and Date: 20130620-23
• [Presentation] Genetic analysis on tame behavior using wild-derived mice (2013)
  • Author(s): Goto, T., Koide, T
  • Organizer: Neuro 2013
  • Place of Presentation: Kyoto
  • Year and Date: 20130620-23
• [Presentation] Genetic dissection of clustered QTLs related to strain difference of home-cage activity (2013)
  • Author(s): Koide, T., Ishii, A., Nishi, A., Umemori, J., Kuriki, S., Kato, S
  • Organizer: Neuro 2013
  • Place of Presentation: Kyoto
  • Year and Date: 20130620-23
• [Presentation] Escalated aggression by the activation of 5-HT system via glutamate in the dorsal raphe nucleus (2013)
  • Author(s): Takahashi, A., Koide, T
  • Organizer: Neuro 2013
  • Place of Presentation: Kyoto
  • Year and Date: 20130620-23
• [Presentation] 野生由来ヘテロジニアスストックを用いたマウス従順性の新たな遺伝解析法の確立 (2013)
  • Author(s): 後藤達彦, 小出剛
  • Organizer: 第60回日本実験動物学会総会
  • Place of Presentation: 筑波
  • Year and Date: 20130515-17
• [Presentation] 野生由来ヘテロジニアスストックマウス作製過程におけるアレル頻度のシミュレーション (2013)
  • Author(s): 小出剛, 高野敏行, 後藤達彦
  • Organizer: 第60回日本実験動物学会総会
  • Place of Presentation: 筑波
  • Year and Date: 20130515-17
• [Presentation] 過剰な攻撃行動を制御する神経メカニズム : 背側縫線核と内側前頭前野 (2013)
  • Author(s): 高橋阿貴, 小出剛
  • Organizer: 第16回神経科学領域における分子モニタリングシンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2013-12-20
• [Presentation] 群飼育マウスにおける上下関係が行動に与える影響 (2013)
  • Author(s): 長澤達弘, 高橋阿貴, 田邉彰, 小出剛, 榊原啓之, 保田倫子, 下位香代子
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-03
• [Presentation] Escalated aggression and the dorsal raphe nucleus : Role of Glutamate and GABA (2013)
  • Author(s): Takahashi A, Miczek KA, Koide T
  • Organizer: SfN 2013
  • Place of Presentation: San Diego, USA
  • Year and Date: 2013-11-09
• [Presentation] Neural control of intermale aggressive behavior of mice : the role of mPFC (2013)
  • Author(s): Takahashi A, Nagayasu K, Kaneko S, Koide T
  • Organizer: Optogenetics2013
  • Place of Presentation: 東京
  • Year and Date: 2013-09-26
• [Presentation] マウスの雄間攻撃行動を制御する神経回路 : 内側前頭前野の役割 (2013)
  • Author(s): 高橋阿貴, 永安一樹, 金子周司, 小出剛
  • Organizer: 日本動物心理学会第73回大会
  • Place of Presentation: 筑波
  • Year and Date: 2013-09-14
• [Presentation] 野生から愛玩化マウスへ : その遺伝的基盤解明に向けて(ワークショップ) (2012)
  • Author(s): 小出剛
  • Organizer: 第84回日本遺伝学会大会ワークショップ「モデル動物の特徴を活かした行動遺伝学の新たな展開」
  • Place of Presentation: 福岡
  • Year and Date: 20120924-26
• [Presentation] 野生由来マウス系統の高い不安様行動に関する遺伝的・分子的解析(口頭) (2012)
  • Author(s): 田邉彰, 高橋阿貴, 城石俊彦, 小出剛
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120918-21
• [Presentation] 神経細胞接着分子 Caspr3 は発達期の大脳基底核に発現する(ポスター) (2012)
  • Author(s): 平田晴菜, 梅森十三, 小出剛, 渡邊和忠, 霜田靖
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120918-21
• [Presentation] マウスの従順性行動に関わる遺伝的影響(ポスター) (2012)
  • Author(s): 後藤達彦, 小出剛
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120918-21
• [Presentation] 背側縫線核 GABAB受容体の活性化が引きおこす過剰な攻撃行動 : グルタミン酸と GABA の役割 (ポスター) (2012)
  • Author(s): 高橋阿貴, 小出剛
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120918-21
• [Presentation] 日本産野生由来マウス系統 MSMにおける高い不安様行動の分子遺伝学的解析 (2012)
  • Author(s): 田邉彰, 石井亜矢子, 城石俊彦, 高橋阿貴, 小出剛
  • Organizer: 第82回日本実験動物学会総会
  • Place of Presentation: 別府
  • Year and Date: 20120524-26
• [Presentation] マウスにおける社会的親和性の遺伝学的解析(シンポジウム) (2012)
  • Author(s): 小出剛
  • Organizer: 第82回日本実験動物学会総会シンポジウム「動物の社会行動解析からヒトの精神疾患を考える」
  • Place of Presentation: 別府
  • Year and Date: 20120524-26
• [Presentation] Genetic and molecular analysis of high anxiety-like behaviors in wild-derived mouse strains (2012)
  • Author(s): Tanave, A., Takahashi, A., Shiroishi, T., Koide, T
  • Organizer: 14th Annual Meeting for International Behavioural and Neural Genetics Sociaty
  • Place of Presentation: Boulder, USA
  • Year and Date: 20120515-19
• [Presentation] Genetic dissection of clustered QTLs related to strain difference of home-cage activity (2012)
  • Author(s): Ishii, A., Nishi, A., Shiroishi, T., Takahashi, A., Koide, T
  • Organizer: 14th Annual Meeting for International Behavioural and Neural Genetics Sociaty
  • Place of Presentation: Boulder, USA
  • Year and Date: 20120515-19
• [Presentation] 野生マウス系統 MSMの過剰な攻撃行動に関わる遺伝的基盤(ポスター) (2012)
  • Author(s): 高橋阿貴, 小出剛
  • Organizer: 第72回日本動物心理学会大会
  • Place of Presentation: 西宮
  • Year and Date: 20120512-13
• [Presentation] Genetic basis of strain difference in waveforms of male ultrasonic vocalization (2012)
  • Author(s): Koide, T., Kikusui, T., Sugimoto, H
  • Organizer: 1st Workshop of Mouse Ultrasonic Communication
  • Place of Presentation: Paris, France
  • Year and Date: 20120416-17
• [Presentation] マウス求愛歌の行動学的解析(シンポジウム) (2011)
  • Author(s): 菊水健史, 小出剛
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 20110914-17
• [Presentation] ドーパミントランスポーター発現制御候補因子 Hesr1/2 ノックアウトマウスの行動解析(ポスター) (2011)
  • Author(s): 菅野康太, 小久保博樹, 相賀裕美子, 高橋阿貴, 小出剛, 石浦章一
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 20110914-17
• [Presentation] 時間依存的な自発活動量のマウス系統差に関わる多因子の遺伝学的解析(ポスター) (2011)
  • Author(s): 後藤達彦, 石井亜矢子, 西明紀, 高橋阿貴, 城石俊彦, 小出剛
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 20110914-17
• [Presentation] MSM-B6 コンソミック系統を用いた不安様行動の分子遺伝学的解析(ポスター) (2011)
  • Author(s): 小出剛, 高橋阿貴, 城石俊彦, 田邉彰
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 20110914-17
• [Presentation] アセチルコリン受容体調節因子 Lynx1 の社会行動への関与(ポスター) (2011)
  • Author(s): 杉本大樹, 高橋阿貴, 小出剛
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 20110914-17
• [Presentation] 背側縫線核 GABAB 受容体の活性化による過剰な攻撃行動の詳細な行動学的・神経学的解析(ポスター) (2011)
  • Author(s): 高橋阿貴, Klaus Miczek, 小出剛
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 20110914-17
• [Presentation] 隠れマルコフモデルを用いたマウス社会行動解析ソフトウェアの開発(ポスター) (2011)
  • Author(s): 田邉彰, 高橋阿貴, 荒川俊也, 柿原聡, 木村真吾, 杉本大樹, 城石俊彦, 富原一哉, 土谷隆, 小出剛
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 20110914-17
• [Presentation] 野生由来マウス系統を用いた行動遺伝学(シンポジウム) (2011)
  • Author(s): 小出剛
  • Organizer: 第58回日本実験動物学会総会
  • Place of Presentation: 東京
  • Year and Date: 20110525-27
• [Presentation] マウス交配行動時の超音波発声の役割(口頭発表) (2011)
  • Author(s): 杉本大樹, 菊水健史, 城石俊彦, 小出剛
  • Organizer: 第58回日本実験動物学会総会
  • Place of Presentation: 東京
  • Year and Date: 20110525-27
• [Presentation] 日本産野生由来マウス系統 MSM の過剰な攻撃行動とセロトニン受容体 mRNA の系統差 (口頭発表) (2011)
  • Author(s): 高橋阿貴, 城石俊彦, 小出剛
  • Organizer: 第58回日本実験動物学会総会
  • Place of Presentation: 東京
  • Year and Date: 20110525-27
• [Presentation] A role of strain difference in waveforms of male ultrasonic vocalization for social behavior (2011)
  • Author(s): Sugimoto, H., Okabe, S., Kikusui, T., Koide, T
  • Organizer: 13th Annual Meeting for International Behavioural and Neural Genetics Sociaty
  • Place of Presentation: Rome, Italy
  • Year and Date: 20110510-14
• [Presentation] Wild-derived stock of mice, a useful resource for studying genetic basis of anxiety-like behavior (2011)
  • Author(s): Koide, T., Tanave, A., Sugimoto, H., Takahashi, A
  • Organizer: 13th Annual Meeting for International Behavioural and Neural Genetics Sociaty
  • Place of Presentation: Rome, Italy
  • Year and Date: 20110510-14
• [Remarks]
  • URL: http://www.nig.ac.jp/labs/MGRL/