2012 Fiscal Year Final Research Report
Development of local cell stimulation method using ultrasound exposure in presence of microbubbles
| Project/Area Number |
23650247
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Hokkaido University |
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Principal Investigator |
KUDO Nobuki 北海道大学, 大学院・情報科学研究科, 准教授 (30271638)
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| Research Collaborator |
OKUYAMA Manabu 北海道大学, 大学院・情報科学研究科, 修士課程学生
KINOSHITA Yuto 北海道大学, 大学院・情報科学研究科, 修士課程学生
MATSUI Tomoko 北海道大学, 大学院・情報科学研究科, 修士課程学生
MIZUUCHI Misato 北海道大学, 大学院・情報科学研究科, 修士課程学生
YOSHIMATSU Yuri 北海道大学, 大学院・情報科学研究科, 修士課程学生
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| Project Period (FY) |
2011 – 2012
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| Keywords | 細胞骨格 / メカノトランスダクション / 細胞局所機械刺激 / 心筋細胞 / 超音波 / 光ピンセット / 微小細胞 |
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| Research Abstract |
A novel technique for local mechanical stimulation of cells was proposed to studymechanotransduction of living cells. A cell with an attached micron-sized bubble is stimulated by this techniqueby using the effect of larger acoustic radiation force being applied to the bubble than to the cell. Optical tweezerswere used to control the position of a bubble. Cardiac myocytes isolated from neonatal rats were used formechanical stimulation, and a cell culture chamber for microscopic observation under exposure of ultrasoundexposure was developed. Cardiac myocytes with attached microbubbles were irradiated with ultrasound, and thegeneration of premature ventricular contraction by the mechanical stimulation was confirmed. Experiments toevaluate the refractory period of the myocytes were carried out using this technique, and changes in the perioddepending on the beating frequency were found, suggesting the usefulness of the proposed technique for studying cell reactions to mechanical stimulation.
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[Journal Article] Prophylactic immunization with Bubble liposomes and ultrasound-treated dendritic cells provided a four-fold decrease in the frequency of melanoma lung metastasis2012
Author(s)
Oda Y, Suzuki R, Otake S, Nishiie N, Hirata K, Koshima R, Nomura T, Utoguchi N, Kudo N, Tachi bana K, and Mar uyama K.
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Journal Title
Journal of Controlled Release
Volume: 160(2)
Pages: 362-6
Peer Reviewed
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