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2013 Fiscal Year Final Research Report

Development of aging inspection system based optical property of collagen

Research Project

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Project/Area Number 23650260
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biological material science
Research InstitutionOsaka University

Principal Investigator

ARAKI Tsutomu  大阪大学, 基礎工学研究科, 教授 (50136214)

Co-Investigator(Kenkyū-buntansha) FUKUSHIMA Shuichiro  大阪大学, 大学院・基礎工学研究科, 助授 (40362644)
Project Period (FY) 2011 – 2012
Keywords老化 / 蛍光 / コラーゲン / 皮膚 / 分子間架橋
Research Abstract

Aging is unavoidable phenomenon in human body. In aged persons, their collagen tissue such as the skin is harder than that in young ones. The collagen reacts with blood glucose non-enzymatically, resulting in production of advanced glycosylation endroducts (AGEs). The AGEs bind to collagen and acts as a cross-link between collagen fibrils, and resultant cross-link causes achage of mechanical property of the collagen tissue.
Aim of this research project is to develop a simple and effective method that can sense AGEs optically. For this purpose, we have focused on nanosecond time-resolved fluorescence of AGEs. Observed fluorescence lifetime of collagen tissue informs degree of accumulation of AGEs in the tissue. To cofirm the accumulation of AGEs, we also carried out immuno-staining of AGE. Using these technique we have examined the aging of dentinal collagen.

  • Research Products

    (9 results)

All 2014 2013 2012 Other

All Journal Article (4 results) (of which Peer Reviewed: 3 results) Presentation (4 results) Remarks (1 results)

  • [Journal Article] Accumulation of advanced glycation end-products in human dentin by aging2014

    • Author(s)
      Jiro Miura, Kantaro Nishikawa, Mizuho Kubo, Shuichiro Fukushima, Mamoru Hashimoto, Fumio Takeshige, and Tsutomu Araki
    • Journal Title

      Arch. Oral Biol

      Volume: Vol.59,No.2 Pages: 119-124

    • Peer Reviewed
  • [Journal Article] In vivo observation of age-related structural changes of dermal collagen in human facial skin using collagen-sensitive second harmonic generation microscope equipped with 1250-nm mode-locked Cr : Forsterite laser2013

    • Author(s)
      Takeshi Y asui, Shuichiro Fukushima, Tsutomu Araki, et al
    • Journal Title

      J. Biomed. Opt

      Volume: Vol.18,No.3 Pages: 031108

    • Peer Reviewed
  • [Journal Article] In vivo visualization of dermal collagen fiber in skin burn by collagen-sensitive second-harmonic-generation microscopy2013

    • Author(s)
      Ryosuke Tanaka, Shuichiro Fukushima, Tsutomu Araki, Takeshi Y asui, et., al
    • Journal Title

      J. Biomed. Opt

      Volume: Vol.18,No.6 Pages: 061231

    • Peer Reviewed
  • [Journal Article] SHG イメージングの組織診断への応用2013

    • Author(s)
      福島修一郎, 橋本守, 荒木勉
    • Journal Title

      京府医大誌

      Volume: Vol.122,No.4 Pages: 189-198

  • [Presentation] Non-staing, in situ visualization of collagen by femtosend laser light and its application to tissuediagnosis2012

    • Author(s)
      Tsutomu Araki
    • Organizer
      2nd International Anatomical Sciences and Cell Biology 3.00 (N/mm) 3.00 (N/mm) Conference
    • Place of Presentation
      Chiang Mai(タイ)
    • Year and Date
      20121206-08
  • [Presentation] 糖化によるヒト象牙質の蛍光と力学特性の変化2012

    • Author(s)
      西川貫太郎
    • Organizer
      第23回バイオフロンティア講演会
    • Place of Presentation
      弘前市
    • Year and Date
      20121005-06
  • [Presentation] Influence of glycosylation on fluorescence characteristics and mechanical properties of human dentin2012

    • Author(s)
      Jiro Miura
    • Organizer
      1st International Conference on Fluorescence-based Diagnostic of Oral Diseases
    • Place of Presentation
      Montpellier(フランス)
    • Year and Date
      20120904-05
  • [Presentation] カニクスとバイオロジーとフォトニクス, あわせてメカノバイオフォトニクス ーその重要性と可能性ー2012

    • Author(s)
      荒木勉
    • Organizer
      第24回バイオエンジニアリング講演会
    • Place of Presentation
      豊中市
    • Year and Date
      2012-01-08
  • [Remarks] 荒木研究室ホームページ

    • URL

      http://sml.me.es.osaka-u.ac.jp/

URL: 

Published: 2015-06-25  

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