2013 Fiscal Year Final Research Report
Development of cell culture methods using sea cucumber collagen fibrils
| Project/Area Number |
23650294
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kogakuin University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SHIN Yong Chol 工学院大学, 工学部, 准教授 (20291734)
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| Project Period (FY) |
2011 – 2013
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| Keywords | コラーゲン / コラーゲン線維 / 細胞培養 / ナマコ / 血管内皮細胞 / 骨形成 / 管腔形成 / アパタイト |
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| Research Abstract |
We have developed newer methods to culture cells by using sea cucumber collagen fibrils as culture substrates. Various conditions were examined for extraction and purification of the collagen fibrils from the body wall dermis in the animal and it became possible to use the fibrils for cell culture. Chemical properties were also demonstrated by showing micro morphology of the fibrils and factors affecting interactions between the fibrils. Some higher order aggregates from the collagen fibrils were formed on the basis of our knowledge about the inter-fibrillar interaction. The collagen fibrils were found to be a good substrate for cell adhesion with various cells examined. Cellular clumps or cellular networks such as capillary like structure were formed under the new culture condition. The collagen fibrils promoted bone differentiation demonstrated by the deposition of apatite with cell culture system. The result indicates the unique surface property of the collagen fibrils.
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