Identification of possible biomarkers for the efficient physiotherapy after the CNS injury.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23650327
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Rehabilitation science/Welfare engineering
• Research Institution: Kyushu University
• Principal Investigator: OKADA SEIJI 九州大学, 医学(系)研究科(研究院), 准教授 (30448435)
• Project Period (FY): 2011 – 2013
• Keywords: リハビリテーション医学 / ギガシークエンサー

Research Abstract

To quantify the efficacy of physiotherapy after the central nervous system injury, we evaluate gene expression changes between before and after rehabilitation/disuse by combining selective nuclear/cellular sorting of motor neurons and transcriptome analysis using next-generation sequencer. After the complete transection of spinal cord at mid-thoracic level, we collected motor neurons located in the level of lumbar intumescence with laser micro dissection at three months after injury, and performed gene expression analysis. This method will contribute to identify the possible biomarkers for establishment of efficient rehabilitation programs.

Research Products

• [Journal Article] Ly6C+Ly6Gmyeloid-derived suppressor cells play a critical role in the resolution of acute inflammation and the subsequent tissue repair process after spinal cord injury (2013)
  • Author(s): Saiwai H, Kumamaru H, Ohkawa Y, Kubota K, Kobayakawa K, Yamada H, Yokomizo T, Iwamoto Y, Okada S
  • Journal Title: J Neurochem Volume: 125 Pages: 74-88
  • DOI: 10.1111/jnc.12135
• [Journal Article] Dedifferentiated chondrosarcoma of the cervical spine : a case report (2013)
  • Author(s): Matsumoto Y, Takahashi Y, Harimaya K, Nakagawa T, Kawaguchi K, Okada S, Hayashida M, Doi T, Sakamoto Y, Matsunobu T, Oda Y, Iwamoto Y
  • Journal Title: World J Surg Oncol Volume: 11 Pages: 32-37
  • DOI: 10.1186/1477-7819-11-32
• [Journal Article] Disturbance of ribcage development causes progressive thoracic scoliosis : The creation of a nonsurgical structural scoliosis model in mice (2013)
  • Author(s): Kubota K, Doi T, Murata M, Kobayakawa K, Matsumoto Y, Harimaya K, Shiba K, Hashizume M, Iwamoto Y, Okada S
  • Journal Title: J Bone Joint Surg Volume: 95 Pages: e1301-7
  • DOI: 10.2106/JBJS.L.01381
• [Journal Article] The therapeutic activities of engrafted neural stem/progenitor cells are not dormant in the chronically injured spinal cord (2013)
  • Author(s): Kumamaru H, Kobayakawa K, Saiwai H, Kubota K, Yokota K, Ohkawa Y, Shiba K, Iwamoto Y, Okada S
  • Journal Title: Stem Cells Volume: 31 Pages: 1535-47
  • DOI: 10.1002/stem.1404
• [Journal Article] Minimum five-year follow up of selective anterior thoracolumbar or lumbar fusion for adolescent idiopathic scoliosis (2013)
  • Author(s): Matsumoto Y, Harimaya K, Doi T, Okada S, Hayashida M, Iwamoto Y
  • Journal Title: J Spine Res Volume: 4 Pages: 933-40
• [Journal Article] Clinical relationship between cervical spinal canal stenosis and traumatic cervical spinal cord injury without major fracture or dislocation (2013)
  • Author(s): Takao T, Morishita Y, Okada S, Maeda T, Katoh F, Ueta T, Mori E, Yugeu I, Kawano O, Shiba K
  • Journal Title: Eur Spine J Volume: 22 Pages: 2228-31
  • DOI: 10.1007/s00586-013-2865-7
• [Journal Article] Hematoma of the ligamentum flavum in the thoracic spine : report of two cases and possible role of the transforming growth factor beta-vascular endothelial growth factor signaling axis in its pathogenesis (2013)
  • Author(s): Matsumoto Y, Fujiwara T, Imamura R, Okada Y, Harimaya K, Doi T, Kawaguchi K, Okada S, Yamada Y, Oda Y, Iwamoto Y
  • Journal Title: J Orthop Sci Volume: 18 Pages: 347-54
  • DOI: 10.1007/s00776-011-0150-3
• [Journal Article] Direct isolation and RNA-seq reveal environment-dependent properties of engrafted neural stem/progenitor cells (2012)
  • Author(s): Kumamaru H, Ohkawa Y, Saiwai H, Yamada H, Kubota K, Kobayakawa K, Akashi K, Okano H, Iwamoto Y, Okada S
  • Journal Title: Nat Commun Volume: 3 Pages: 1140
  • DOI: 10.1038/ncomms2132
• [Journal Article] Analysis of the risk factors for tracheostomy in traumatic cervical spinal cord injury (2012)
  • Author(s): Yugué I, Okada S, Ueta T, Maeda T, Mori E, Kawano O, Takao T, Sakai H, Masuda M, Hayashi T, Morishita Y, Shiba K
  • Journal Title: Spine Volume: 37 Pages: E1633-8
  • DOI: 10.1097/BRS.0b013e31827417f1
• [Journal Article] Soft-tissue damage and segmental instability in adult patients with cervical spinal cord injury without major bone injury (2012)
  • Author(s): Maeda T, Ueta T, Mori E, Yugue I, Kawano O, Takao T, Sakai H, Okada S, Shiba K
  • Journal Title: Spine Volume: 37 Pages: E1560-6
  • DOI: 10.1097/BRS.0b013e318272f345
• [Journal Article] Liposomal clodronate selectively eliminates microglia from primary astrocyte cultures (2012)
  • Author(s): Kumamaru H, Saiwai H, Kobayakawa K, Kubota K, van Rooijen N, Inoue K, Iwamoto Y, Okada S
  • Journal Title: J Neuroinflammation Volume: 9 Pages: 116
  • DOI: 10.1186/1742-2094-9-116
• [Journal Article] Chd2 interacts with H3.3 to determine myogenic cell fate (2012)
  • Author(s): Harada A, Okada S, Konno D, Odawara J, Yoshimi T, Yoshimura S, Kumamaru H, Saiwai H, Tsubota T, Kurumizaka H, Akashi K, Tachibana T, Imbalzano AN, Ohkawa Y
  • Journal Title: EMBO J Volume: 31 Pages: 2994-3007
  • DOI: 10.1038/emboj.2012.136
• [Journal Article] Neurological recovery is impaired by concurrent but not by asymptomatic pre-existing spinal cord compression after traumatic spinal cord injury (2012)
  • Author(s): Kubota K, Saiwai H, Kumamaru H, Kobayakawa K, Maeda T, Matsumoto Y, Harimaya K, Iwamoto Y, Okada S
  • Journal Title: Spine Volume: 37 Pages: 1448-55
  • DOI: 10.1097/BRS.0b013e31824ffda5
• [Journal Article] Bioluminescent system for dynamic imaging of cell and animal behavior (2012)
  • Author(s): Hara-Miyauchi C, Tsuji O, Hanyu A, Okada S, Yasuda A, Fukano T, Akazawa C, Nakamura M, Imamura T, Matsuzaki Y, Okano HJ, Miyawaki A, Okano H
  • Journal Title: Biochem Biophys Res Commun Volume: 419 Pages: 188-93
  • DOI: 10.1016/j.bbrc.2012.01.141
• [Journal Article] Myeloperoxidase exacerbates secondary injury by generating highly reactive oxygen species and mediating neutrophil recruitment in experimental spinal cord injury (2012)
  • Author(s): Kubota K, Saiwai H, Kumamaru H, Maeda T, Ohkawa Y, Aratani Y, Nagano T, Iwamoto Y, Okada S
  • Journal Title: Spine Volume: 37 Pages: 1363-9
  • DOI: 10.1097/BRS.0b013e31824b9e77
• [Journal Article] Spinous process-splitting open pedicle screw fusion provides favorable results in patients with low back discomfort and pain compared to conventional open pedicle screw fixation over 1 year after surgery (2012)
  • Author(s): Mori E, Okada S, Ueta T, Itaru Y, Maeda T, Kawano O, Shiba K
  • Journal Title: Eur Spine J Volume: 21 Pages: 745-53
  • DOI: 10.1007/s00586-011-2146-2
• [Journal Article] Clinical characteristics and surgical outcome of the symptomatic ossification of ligamentum flavum at the thoracic level with combined lumbar spinal stenosis (2012)
  • Author(s): Matsumoto Y, Harimaya K, Doi T, Kawaguchi K, Okada S, Inoguchi A, Fujiwara M, Iwamoto Y
  • Journal Title: Arch Orthop Trauma Surg Volume: 132 Pages: 465-70
  • DOI: 10.1007/s00402-011-1438-7
• [Journal Article] Extradural nodular fasciitis arising in the spinal canal (2012)
  • Author(s): Kubota K, Okada S, Maeda T, Matsumoto Y, Sakamoto A, Harimaya K, Saiwai H, Kumamaru H, Oda Y, Iwamoto Y
  • Journal Title: Spine Volume: 37 Pages: E133-7
  • DOI: 10.1097/BRS.0b013e318224568a
• [Journal Article] Age-related differences in cellular and molecular profiles of inflammatory responses after spinal cord injury (2012)
  • Author(s): Kumamaru H, Saiwai H, Ohkawa Y, Yamada H, Iwamoto Y, Okada S
  • Journal Title: J Cell Physiol Volume: 227 Pages: 1335-46
  • DOI: 10.1002/jcp.22845
• [Journal Article] Generation of a rat monoclonal antibody specific for hsp72 (2011)
  • Author(s): Tanaka M, Shiota M, Okada S, Harada A, Odawara J, Mun S, Iwao H, Ohkawa Y
  • Journal Title: Hybridoma Volume: 30 Pages: 397-400
  • DOI: 10.1089/hyb.2011.0015
• [Journal Article] The classification of mRNA expression levels by the phosphorylation state of RNAPII CTD based on a combined genome-wide approach (2011)
  • Author(s): Odawara J, Harada A, Yoshimi T, Maehara K, Tachibana T, Okada S, Akashi K, Ohkawa Y
  • Journal Title: BMC Genomics Volume: 12 Pages: 516
  • DOI: 10.1186/1471-2164-12-516
• [Journal Article] Beneficial compaction of spinal cord lesion by migrating astrocytes through glycogen synthase kinase-3 inhibition (2011)
  • Author(s): Renault-Mihara F, Katoh H, Ikegami T, Iwanami A, Mukaino M, Yasuda A, Nori S, Mabuchi Y, Tada H, Shibata S, Saito K, Matsushita M, Kaibuchi K, Okada S, Toyama Y, Nakamura M, Okano H
  • Journal Title: EMBO Mol Med Volume: 3 Pages: 682-96
  • DOI: 10.1002/emmm.201100179
• [Journal Article] Macrophage infiltration predicts a poor prognosis for human ewing sarcoma (2011)
  • Author(s): Fujiwara T, Fukushi J, Yamamoto S, Matsumoto Y, Setsu N, Oda Y, Yamada H, Okada S, Watari K, Ono M, Kuwano M, Kamura S, Iida K, Okada Y, Koga M, Iwamoto Y
  • Journal Title: Am J Pathol Volume: 179 Pages: 1157-70
  • DOI: 10.1016/j.ajpath.2011.05.034
• [Presentation] 脊髄損傷に対する治験- ステロイド大量療法の歴史から学ぶ (2013)
  • Author(s): 岡田誠司
  • Organizer: 第48回日本脊髄障害医学会
  • Place of Presentation: 福岡
  • Year and Date: 20131114-15
• [Presentation] Hyperglycemia-induced microglial overactivation deteriorates the secondary injury via NF-κB pathway after spinal cord injury (2013)
  • Author(s): Kobayakawa K, Saiwai H, Kumamaru H, Kubota K, Ohkawa Y, Shiba K, Yokota Y, Iwamoto Y, Okada S
  • Organizer: The 43th Annual Meeting of Society for Neuroscience
  • Place of Presentation: San Diego, USA
  • Year and Date: 20131109-13
• [Presentation] Structural and functional changes of distal site to the lesion after chronic complete spinal cord injury (2013)
  • Author(s): Yokota K, Kobayakawa K, Iwamoto Y, Okada S
  • Organizer: The 43th Annual Meeting of Society for Neuroscience
  • Place of Presentation: San Diego, USA)
  • Year and Date: 20131109-13
• [Presentation] 慢性期脊髄損傷に対する神経幹細胞移植 (2013)
  • Author(s): 岡田誠司, 熊丸浩仁, 小早川和, 横田和也, 斉藤武恭, 岩本幸英
  • Organizer: 第28回日本整形外科学会基礎学術集会
  • Place of Presentation: 千葉
  • Year and Date: 20131017-18
• [Presentation] セルソーターの脊髄再生研究への応用 (2013)
  • Author(s): 岡田誠司, 熊丸浩仁, 林田光正, 松本嘉寛, 播广谷勝三, 岩本幸英
  • Organizer: 第42回日本脊椎脊髄病学会
  • Place of Presentation: 沖縄
  • Year and Date: 20130425-27
• [Presentation] High glucose-induced microglial activation deteriorates the secondary injury via NF-kB pathway after experimental spinal cord injury (2013)
  • Author(s): Kobayakawa K, Kubota K, Iwamoto Y, Okada S
  • Organizer: ORS 2013 Annual Meeting
  • Place of Presentation: San Antonio, USA
  • Year and Date: 20130126-29
• [Presentation] Neurological recovery is impaired by concurrent but not by asymptomatic pre-existing spinal cord compression after traumatic spinal cord injury (2013)
  • Author(s): Kubota K, Doi T, Kobayakawa K, Matsumoto Y, Hariimaya K, Iwamoto Y, Okada S
  • Organizer: ORS 2013 Annual Meeting
  • Place of Presentation: San Antonio, USA
  • Year and Date: 20130126-29
• [Presentation] 脊髄損傷後の自己修復メカニズム (2012)
  • Author(s): 岡田誠司, 熊丸浩仁, 久保田健介, 小早川 和 , 中村雅也 , Renault-Mihara Francois, 岡野栄之, 岩本幸英
  • Organizer: 第27回日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 20121026-27
• [Presentation] 脊髄損傷研究に 於 け る MDSC(myeloid-derived suppressor cell) の生理的修復作用 (2012)
  • Author(s): 岡田誠司, 幸博和, 熊丸浩仁, 久保田健介, 小早川和, 岩本幸英
  • Organizer: 第27回日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 20121026-27
• [Presentation] 脊髄圧迫病変が脊髄損傷の予後に与える影響 (2011)
  • Author(s): 岡田誠司
  • Organizer: 第46回日本脊髄障害医学会
  • Place of Presentation: 大阪
  • Year and Date: 20111118-19
• [Presentation] 損傷脊髄部での移植神経幹細胞の in situ analysis (2011)
  • Author(s): 岡田誠司
  • Organizer: 第46回日本脊髄障害医学会
  • Place of Presentation: 大阪
  • Year and Date: 20111118-19
• [Presentation] Age-related differences in cellular and molecular profiles of inflammatory responses after spinal cord injury (2011)
  • Author(s): Kumamaru H, Saiwai H, Iwamoto Y, Okada S
  • Organizer: The 41th Annual Meeting of Society for Neuroscience
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20111112-16
• [Presentation] Delivery of Ly6C(+) myeloid-derived suppressor cells (MDSCs) promote tissue repair process and functional recovery after spinal cord injury (2011)
  • Author(s): Saiwai H, Kumamaru H, Kubota K, Kobayakawa K, Iwamoto Y, Okada S
  • Organizer: The 41th Annual Meeting of Society for Neuroscience
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20111112-16
• [Presentation] 脊髄損傷研究に於ける網羅的遺伝子解析の応用 (2011)
  • Author(s): 岡田誠司, 熊丸浩仁, 久保田健介, 小早川和, 幸博和, 岩本幸英
  • Organizer: 第26回日本整形外科学会基礎学術集会
  • Place of Presentation: 前橋
  • Year and Date: 20111020-21
• [Book] The mechanism behind functional recovery after the incomplete spinal cord injury. Neuroprotection and Regeration of the Spinal Cord. (In Uchida K, Nakamura M, Ozawa H, Katoh S (eds)) (2014)
  • Author(s): Okada S
  • Total Pages: 3-10
  • Publisher: Springer
• [Remarks]
  • URL: http://hyoka.ofc.kyushu-u.ac.jp/search/details/K003217