2012 Fiscal Year Final Research Report
Study of the memory of genome stress
| Project/Area Number |
23650588
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Carcinogenesis
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| Research Institution | Hiroshima University |
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Principal Investigator |
TASHIRO Satoshi 広島大学, 原爆放射線医科学研究所, 教授 (20243610)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 放射線 / 抗がん剤 / ゲノム損傷 / クロマチン / クロマチン免疫沈降シーケンス法 |
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| Research Abstract |
The purpose of this study is to test whether there is a system to memory the information of genome damage induced by ionizing radiation or anti-cancer drugs in human cells or not. We performed genome-wide analysis of the binding of DNA repair protein RAD51 to chromatin after induction of DNA damage. We could detect the binding of RAD51 to damaged sites and sub-telomeric regions at 2 hours after induction of DNA damage. The RAD51 binding to the damaged sites were reduced at two weeks after induction of DNA damage. We are now examining the binding of other repair factors and histones to the damaged sites at two weeks after induction of DNA damage.
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[Journal Article] Shi L, Fujioka K, Sun J, Kinomura A, Inaba T, Ikura T, Ohtaki M, Yoshida M, Kodama Y, Livingston GK, Kamiya K, Tashiro S2012
Author(s)
Shi L, Fujioka K, Sun J, Kinomura A, Inaba T, Ikura T, Ohtaki M, Yoshida M, Kodama Y, Livingston GK, Kamiya K, Tashiro S
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Journal Title
Radiat Res
Volume: 177(5)
Pages: 533-538
Peer Reviewed
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[Journal Article] Involvement of ribonucleotide reductase-M1 in 5-fluorouracil-induced DNA damage in esophageal cancer cell lines
Author(s)
Aoki Y, Sakogawa K, Hihara J, Emi M, Hmamai Y, Kono K, Shi L, Sun J, Kitao H, Ikura T, Niida H, Nakanishi M, Okada M, Tashiro S
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Journal Title
Int J Oncol
Volume: (in press)
Peer Reviewed
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[Journal Article] A functional deficiency of TERA/VCP/p97 contributes to impaired DNA damage repair in multiple polyglutamine diseases
Author(s)
Fujita K, Nakamura Y, Oka T, Ito H, Tamura T, Tagawa K, Sasabe T, Katsuta A, Motoki K, Shiwaku H, Sone M, Yoshida C, Katsuno M, Sishi Y, Murata M, Taylor JP, Wanker EE, Kono K, Tashiro S, Sobue G, La Spada AR, Okazawa H
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Journal Title
Nature commun
Volume: (in press)
Peer Reviewed
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[Presentation] A new system for analyzing ionizing radiation-induced chromosome abnormalities2011
Author(s)
時林,藤岡来実,孫継英,木野村愛子,稲葉俊哉,井倉毅,大瀧慈,吉田光明,児玉吉明, Gordon K. Livingston, 神谷研二,田代聡
Organizer
第34回日本分子生物学会年会
Place of Presentation
横浜
Year and Date
2011-12-13
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[Presentation] A new system for analyzing ionizing radiation-induced chromosome abnormalities2011
Author(s)
時林,藤岡来実,孫継英,木野村愛子,稲葉俊哉,井倉毅,大瀧慈,吉田光明,児玉吉明, Gordon K. Livingston,神谷研二,田代聡
Organizer
日本放射線影響学会 第54回大会
Place of Presentation
神戸
Year and Date
2011-11-17
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[Presentation] A new system for analyzing ionizing radiation-induced chromosome abnormalities2011
Author(s)
時林,藤岡来実,孫継英,木野村愛子,稲葉俊哉,井倉毅,大瀧慈,吉田光明,児玉吉明, Gordon K. Livingston, 神谷研二, 田代 聡
Organizer
第84回日本生化学会大会
Place of Presentation
京都
Year and Date
2011-09-21
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