Development and characterization of cancer stem cells from iPS cells

2013 Fiscal Year Final Research Report

• Project/Area Number: 23650598
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: Okayama University
• Principal Investigator: SENO Masaharu 岡山大学, 自然科学研究科, 教授 (90243493)
• Co-Investigator(Kenkyū-buntansha): MIZUTANI Akifumi 岡山大学, 大学院・自然科学研究科, 助教 (50598331) ; KUDOH Takayuki 岡山大学, 大学院・自然科学研究科, 助教 (00346412)
• Project Period (FY): 2011 – 2013
• Keywords: 幹細胞 / iPS細胞 / がん幹細胞 / 微小環境 / 分化 / 培養上清

Research Abstract

It is widely recognized that cancer stem cells (CSCs) are responsible for initiation, metastasis, and recurrence of cancer. The lack of suitable model of CSCs was one of the limiting factors for the progress of CSC research while, for complete cure of cancer, CSCs are considered most important therapeutic targets. In this study, we have succeeded in establishing model cell lines of CSCs, into which was converted from iPS cells cultured under the cancerous conditions.

Research Products

• [Journal Article] Mouse induced pluripotent stem cell microenvironment generates epithelial-mesenchymal transition in mouse Lewis lung cancer cells (2014)
  • Author(s): Chen L, Mizutani A, Kasai T, Yan T, Jin G, Vaidyanath A, El-Aarag BY, Liu Y, Kudoh T, Salomon DS, Fu L, Seno M
  • Journal Title: Am J Cancer Res Volume: 15; 4(1) Pages: 80-8
  • URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3902235/
  • Peer Reviewed
• [Journal Article] がん幹細胞から考えるがんの正体 -クローン説に基づく治療薬/治療法からのパラダイムシフト (2014)
  • Author(s): 妹尾昌治, 笠井智成, 村上宏, 水谷昭文
  • Journal Title: 化学 Volume: vol.69, No.3 Pages: 70-71
• [Journal Article] Cancer stem cells maintain a hierarchy of differentiation by creating their niche (2014)
  • Author(s): Matsuda S, Yan T, Mizutani A, Sota T, Hiramoto Y, Prieto-Vila M, Chen L, Satoh A, Kudoh T, Kasai T, Murakami H, Fu L, Salomon DS, Seno M
  • Journal Title: Int J Cancer Volume: 135(1) Pages: 27-36
  • DOI: 10.1002/ijc.28648.Epub2013Dec9
  • Peer Reviewed
• [Journal Article] 【My Technology】マウスiPS 細胞から作るがん幹細胞モデル (2013)
  • Author(s): 笠井智成, 陳 凌, 工藤孝幸, 水谷昭文, 妹尾昌治
  • Journal Title: 細胞工学(秀潤社) Volume: Vol.32(No.3) Pages: 330-337
• [Journal Article] A model of cancer stem cells derived from mouse induced pluripotent stem cells (2012)
  • Author(s): Chen L, Kasai T, Li Y, Sugii Y, Jin G, Okada M, Vaidyanath A, Mizutani A, Satoh A, Kudoh T, Hendrix MJ, Salomon DS, Fu L, Seno M
  • Journal Title: PLoS One Volume: 7(4) Pages: e33544
  • DOI: 10.1371/journal.pone.0033544.Epub
  • Peer Reviewed
• [Presentation] Our cancer stem cell project : past, present and future (2014)
  • Author(s): Seno M
  • Organizer: 第7回高度医療都市を創出する未来技術国際シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2014-02-07
• [Presentation] Cancer stem cells maintain a hierarchy of differentiation by creating their niche (2014)
  • Author(s): Matsuda S, Yan T, Mizutani A, Murakami H, Seno M
  • Organizer: 第7回高度医療都市を創出する未来技術国際シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2014-02-07
• [Presentation] Characterization of cancer stem-like cell converted from mouse iPS cell by tumor derived exosome/microvesicle (2013)
  • Author(s): Yan T, Mizutani A, Chen L, Hiramoto Y, Takaki M, Murakami H, Seno M
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 20131203-06
• [Presentation] In vitro niche created by cancer stem-like cells derived from mouse induced pluripotent stem cell (2013)
  • Author(s): Mizutani A, Matsuda S, Yan T, Sota T, Kasai T, Murakami H, Kudoh T, Seno M
  • Organizer: American Association for Cancer Research Annual Meeting 2013
  • Place of Presentation: アメリカ ワシントンDC
  • Year and Date: 20130406-10
• [Presentation] Cancer stem cells derived from mouse iPS (2013)
  • Author(s): Seno M
  • Organizer: 日印再生医療センター第8回年次大会
  • Place of Presentation: インド
  • Year and Date: 2013-10-19
• [Presentation] Cancer stem-like cells derived from mouse induced pluripotent stem cell create a niche to promote self-renewal by themselves (2012)
  • Author(s): Yan T, Matsuda S, Prieto Vila M, Mizutani A. Murakami H, Seno M
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 20121211-14
• [Presentation] マウス人工多能性幹細胞より誘導したがん幹細胞モデルの生体外解析 (2012)
  • Author(s): 松田修一, 水谷昭文, 笠井智成, 佐藤あやの, 工藤孝幸, 陳凌, 妹尾昌治
  • Organizer: 日本癌学会 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 20120919-21
• [Presentation] Development and characterization of cancer stem cell model from mouse iPS cells (2012)
  • Author(s): Chen L, Matsuda S, Kasai T, Sugii Y, Okada M, Igarashi K, Satoh A, Kudoh T, Fu L, Seno M
  • Organizer: American Association for Cancer Research, AACR annual meeting 2012
  • Place of Presentation: シカゴ
  • Year and Date: 20120331-0404
• [Presentation] iPS 細胞から作るがん幹細胞モデル : がん幹細胞を誘導する微小環境へのアプローチ (2012)
  • Author(s): 妹尾昌治
  • Organizer: 第13回バイオメディカル研究会
  • Place of Presentation: 大阪
  • Year and Date: 2012-10-26
• [Book] Chapter 6 A cancer stem cell model : an insight into the conversion of induced pluripotent stem cells to cancer stem-likecells. In "Cancer Stem Cells (Ed. Rajasekhar K. Vinagolu)" (2014)
  • Author(s): Mizutani A, Chen L, Kasai T, Kudoh T, Murakami H, Fu L, Seno M
  • Total Pages: 79-88
  • Publisher: Wiley-Blackwell
• [Book] Cancer stem cells derived from mouse induced pluripotent stem cells. In "Stem cells and cancer stem cells Volume 11 (Ed. M.A. Hayat)" (2014)
  • Author(s): Murakami H, Mizutani A, Chen L, Kasai T, Kudoh T, Fu L, Seno M
  • Total Pages: 127-133
  • Publisher: Springer
• [Remarks] 本研究は, クローズアップ現代(HK, 2012.7.2), 真相報道バンキシャ (日本テレビ, 2012.10.14) その他, 中国地区ローカルニュース, 全国紙を含む約20 紙で取り上げられた.