2012 Fiscal Year Final Research Report
Novel drug delivery system using human iPS cell technology for recognition of cancer-related antigen
| Project/Area Number |
23650622
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Clinical oncology
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| Research Institution | Kyoto University |
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Principal Investigator |
ETO Koji 京都大学, iPS 細胞研究所, 教授 (50286986)
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| Project Period (FY) |
2011 – 2012
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| Keywords | iPS 細胞 / 腫瘍がん抗原 / 血小板 / T リンパ球 |
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| Research Abstract |
Original aim of this study is to achieve the proof of concept for drug delivery system (DDS) using iPS cell technology. Idea is derived from the granule release in platelets (dense- or alpha-granule in platelets), whereby engineered megakaryocytes that express rearranged specific T cell receptor component to cancer-related antigen may be able to make unique type of DDS platelets. In that context, we have established T lymphocyte generation, particularly CD8 cytotoxic cells in addition to naive T cells from original antigen-specific CD8 T cells through iPS cells in human system (Cell Stem Cell, 2013). We also showed that iPS cell-derived megakaryocytes are capable of expressing TCR component, contributing to original concept in the future.
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[Journal Article] Generation of Rejuvenated Antigen-Specific T Cells by Reprogramming to Pluripotency and Redifferentiation2013
Author(s)
Nishimura T, Kaneko S, Kawana-Tachikawa A, Tajima Y, Goto H, Zhu D, Nakayama-Hosoya K, Iriguchi S, Uemura Y, Shimizu T, Takayama N, Yamada D, Nishimura K, Ohtaka M, Watanabe N, Takahashi S, Iwamoto A, Koseki H, Nakanishi M, Eto K, Nakauchi H
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Journal Title
Cell Stem Cel
Volume: 12(1)
Pages: 114-26
DOI
Peer Reviewed
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[Journal Article] Role of SOX17 in hematopoietic development from human embryonic stem cells2013
Author(s)
Nakajima-Takagi Y, Osawa M, Oshima M, Takagi H, Miyagi S, Endoh M, Endo TA, Takayama N, Eto K, Toyoda T, Koseki H, Nakauchi H, Iwama A
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Journal Title
Blood
Volume: 121(3)
Pages: 447-58
DOI
Peer Reviewed
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[Journal Article] Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cells2012
Author(s)
Kajiwara M, Aoi T, Okita K, Takahashi R, Inoue H, Takayama N, Endo H, Eto K, Toguchida J, Uemoto S, Yamanaka S
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Journal Title
Proc Natl Acad Sci U S A
Volume: 109(31)
Pages: 12538-43
DOI
Peer Reviewed
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[Journal Article] Generation of induced pluripotent stem cells from primary chronic myelogenous leukemia patient samples2012
Author(s)
Kumano K, Arai S, Hosoi M, Taoka K, Takayama N, Otsu M, Nagae G, Ueda K, Nakazaki K, Kamikubo Y, Eto K,Aburatani H, Nakauchi H, Kurokawa M
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Journal Title
Blood
Volume: 119(26)
Pages: 6234-42
DOI
Peer Reviewed
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[Journal Article] Integrin αvβ3 regulates thrombopoietin-mediated maintenance of hematopoietic stem cells2012
Author(s)
Umemoto T, Yamato M, Ishihara J, Shiratsuchi Y, Utsumi M, Morita Y, Tsukui H, Terasawa M, Shibata T, Nishida K, Kobayashi Y, Petrich BG, Nakauchi H, *Eto K, Okano T
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Journal Title
Blood
Volume: 119(1)
Pages: 83-94
Peer Reviewed
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[Journal Article] In vivo imaging visualizes discoid platelet aggregations without endothelium disruption and implicates contribution of inflammatory cytokine and integrin signaling2012
Author(s)
Nishimura S, Manabe I, Nagasaki M, Kakuta S, Iwakura Y, Takayama N, Ooehara J, Otsu M, Kamiya A, Petrich B, Urano T, Kadono T, Sato S, Aiba A, Yamashita H, Sugiura S, Kadowaki T, Nakauchi H, *Eto K, Nagai R
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Journal Title
Blood
Volume: 119(8)
Pages: e45-56
DOI
Peer Reviewed
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[Journal Article] Heterozygous ITGA2B R995W mutation inducing constitutive activation of the {alpha}IIb{beta}3 receptor affects proplatelet formation and causes congenital macrothrombocytopenia2011
Author(s)
Kunishima S, Kashiwagi H, Otsu M, Takayama N, Eto K, Onodera M, Miyajima Y, Takamatsu Y, Suzumiya J, Matsubara K, Tomiyama Y, Saito H
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Journal Title
Blood
Volume: 117
Pages: 5479-84
Peer Reviewed
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[Presentation] Generation and analysis of induced pluripotent stem derived from secondary myelofibrosis cells2012
Author(s)
Masataka Hosoi, Keiki Kumano, Kazuki Taoka, Shunya Arai, Koki Ueda, Yasuhiko Kamikubo, Naoya Takayama, Makoto Otsu, Koji Eto, Hiromitsu Nakauchi, Mineo Kurokawa
Organizer
第74回日本血液学会学術集会
Place of Presentation
京都
Year and Date
20121019-21
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[Presentation] Suppressed Neutrophil Development in Hematopoiesis of Induced Pluripotent Stem Cells Derived From a Severe Congenital Neutropenia Patient with ELA2 Mutation2011
Author(s)
Takafumi Hiramoto, Yasuhiro Ebihara, Yoko Mizoguchi, Kazuhiro Nakamura, Shinji Mochizuki, Shohei Yamamoto, Emiko Matsuzaka, Sachiyo Hanada, Ryoko Ohnishi, Kenzaburo Tani, Koji Eto, Hiromitsu Nakauchi, Masao Kobayashi, and Kohichiro Tsuji
Organizer
53rd ASH Annual Meeting and Exposition
Place of Presentation
San Diego, USA
Year and Date
20111210-13
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[Presentation] Modeling Congenital Amegakaryocytic Thrombocytopenia Using Patient-Specific Induced Pluripotent Stem Cells2011
Author(s)
Naoya Takayama, Shinji Hirata, Ryoko Jono-Ohnishi, Sou Nakamura, Sho-ichi Hirose, Hiroshi Endo, Masanori Nishi, Yuhei Hamazaki, Shin Kaneko, Eiichi Ishii, Hiromitsu Nakauchi, Shinji Kunishima, and Koji Eto
Organizer
53rd ASH Annual Meeting and Exposition
Place of Presentation
San Diego, USA
Year and Date
20111210-13
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[Presentation] TRANSIENT ACTIVATION OF C-MYC EXPRESSION IS CRITICAL FOR EFFICIENT PLATELET GENERATION FROM HUMAN INDUCED PLURIPOTENT STEM CELLS2011
Author(s)
Takayama N, Nishimura S, Nakamura S, Shimizu T, Ohnishi R, Endo H,Yamaguchi T, Otsu M, Nishimura K, Nakanishi M, Sawaguchi A, Nagai R, Takahashi K, Yamanaka S, Nakauchi H, Eto K
Organizer
XXIII Congress of the International Society on Thrombosis and Haemostasis(ISTH2011)
Place of Presentation
Kyoto, Japan
Year and Date
20110723-28
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