2012 Fiscal Year Final Research Report
Regulation of protein expression using a cell cyclce-specific degron and its application to functional analysis
| Project/Area Number |
23651046
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Kyoto University |
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Principal Investigator |
TAKATA Minoru 京都大学, 放射線生物研究センター, 教授 (30281728)
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| Project Period (FY) |
2011 – 2012
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| Keywords | DNA修復 / DNA損傷チェックポイント / Treslin / Geminin / Cdt1 / 細胞周期特異的デグロン |
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| Research Abstract |
FUCCIis a recently developed cell cycle specific fluorescent maker. This is based on the cell cycle dependent degradation of Cdt1 or Geminin proteins induced by polyubiquitination. We reasoned this can be exploited if we fuse a part of Cdt1 or Geminin used in FUCCI with a protein forcontrolingexpression in a cell cycle specific manner. This would be most useful when used in combination with powerful genetics in chicken DT40 cell system. Thus we cloned chicken Cdt1 or Geminin thatcorresponds to part of human Cdt1 or Geminin used for FUCCI. However, we could not achieve high expression levels in wild type DT40 cells. We havecreated a cell line rendered conditionally deficient in the Treslin gene, which is a yeast Sld3 homologessential for intiation of DNA replication.This cell line would be useful to study Treslin function.
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Research Products
(23 results)
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[Journal Article] Histone chaperone activity of Fanconi anemiaproteins, FANCD2 and FANCI, is required for DNA crosslink repair2012
Author(s)
Sato K, Ishiai M, Toda K, Furukoshi S, Osakabe A, Tachiwana H, Takizawa Y, Kagawa W, Kitao H, Dohmae N, Obuse C, Kimura H, Takata M, Kurumizaka H
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Journal Title
EMBO J
Volume: 31(17)
Pages: 3524-36
DOI
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[Journal Article] A ubiquitin-binding protein, FAAP20, links RNF8-mediated ubiquitination to the Fanconi anemiaDNA repair network2012
Author(s)
Yan Z, Guo R, Paramasivam M, Shen W, Ling C, Fox D 3rd, Wang Y, Oostra AB, Kuehl J, Lee DY, Takata M, Hoatlin ME, Schindler D, Joenje H, de Winter JP, Li L, Seidman MM, Wang W
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Journal Title
Mol Cell
Volume: 47(1)
Pages: 61-75
DOI
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[Journal Article] ATR-ATRIP kinase complex triggers activation of the Fanconianemia DNA repair pathway2012
Author(s)
Shigechi T, Tomida J, Sato K, Kobayashi M, Eykelenboom JK, Pessina F, Zhang Y, Uchida E, Ishiai M, Lowndes NF, Yamamoto K, Kurumizaka H, Maehara Y, Takata M
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Journal Title
Cancer Res
Volume: 72(5)
Pages: 1149-1156
DOI
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[Journal Article] Predisposition to cancer caused by genetic and functional defects of Mammalian atad52011
Author(s)
BellDW, Sikdar N, Lee KY, Price JC, Chatterjee R, Park HD, Fox J, Ishiai M, Rudd ML, Pollock LM, Fogoros SK, Mohamed H, Hanigan CL; NISC Comparative Sequencing Program, Zhang S, Cruz P, Renaud G, Hansen NF, Cherukuri PF, Borate B, McManus KJ, Stoepel J, S
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Journal Title
PLoS Genet
Volume: 7(8)
Pages: e1002245
DOI
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[Journal Article] ATR-Chk1 signaling pathway and homologous recombinational repair protect cells from 5-fluorouracil cytotoxicity2011
Author(s)
FujinakaY, Matsuoka K, Iimori M, Tuul M, Sakasai R, Yoshinaga K, Saeki H, Morita M, Kakeji Y, Gillespie DA, Yamamoto KI, Takata M, Kitao H, Maehara Y
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Journal Title
DNA Repair (Amst)
Volume: 11(3)
Pages: 247-58
DOI
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