Mechanizme analysis of accumulation of PFOS in blood

2012 Fiscal Year Final Research Report

• Project/Area Number: 23651053
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kyushu University
• Principal Investigator: OSHIMA Yuji 九州大学, 農学研究院, 教授 (70176874)
• Co-Investigator(Kenkyū-buntansha): 島崎 洋平 九州大学, 農学研究院 (40363329)
• Project Period (FY): 2011 – 2012
• Keywords: PFOS 結合タンパク質 / 魚類 / 血液

Research Abstract

Perfluorooctane sulfonate (PFOS) was known to accumulate in blood of fish, bird and mammals. PFOS might bind with unknown substance in blood, however the mechanism is unclear. Thus, we performed to purify a PFOS binding substance from fish blood and confirmed its affinity to PFOS. As results, PFOS binding protein was successfully purified from the plasma of PFOS administered-puffer fish (Takifugu rubripes) from the 65-70% ammonium sulfate precipitation. N-terminal analysis strongly suggested that PFOS-binding protein was an apolipoprotein A-I in T. rubripes. In accorded this, PFOS was confirmed to bind with the 65-70% ammonium sulfate fraction prepared from non-PFOS treated T. rubripes. Those results indicated that PFOS was bind to apolipoprotein A-I. Thus, this may suggest disorder of physiological function because apolipoprotein A-I may play an important role in lipid metabolism in fish.