2012 Fiscal Year Final Research Report
Investigationof mechanisms of radical oxygen species (ROS) via ATP・ATP receptor signaling in the cells exposed to nanoparticles
| Project/Area Number |
23651055
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
KOJIMA Shuji 東京理科大学, 薬学部, 教授 (90119579)
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| Project Period (FY) |
2011 – 2012
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| Keywords | ナノ粒子 / 活性酸素 / 環境科学 / ATP受容体 / 細胞毒 |
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| Research Abstract |
The mechanism of ROS production via ATP-ATP signaling was examined in the mouse kidney mesangium cells exposed to nano-silica particles with 30, 70, and 300nm of diameter (nSP30, nSP70, and nSP300, respectively). As a result, the significant ATP release, Ca2+influx and ROS production were observed in the cells exposed to nSP30 and nSP70. These events were blocked by the treatment with ecto-nucleotidase apyrase, suggesting involvement of ATP・ATP receptor signaling in nSP-induced ROS production. Furthermore, the same results were obtained in human keratinocytes, HaCaT cells.
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