2012 Fiscal Year Final Research Report
Chemical Developmental Toxicity Test Using A New Material Introducing Technique for Medaka Egg
| Project/Area Number |
23651057
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Ariake National College of Technology |
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Principal Investigator |
TOMINAGA Nobuaki 有明工業高等専門学校, 物質工学科, 教授 (30227631)
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| Co-Investigator(Kenkyū-buntansha) |
KONO Susumu 有明工業高等専門学校, 電気工学科, 教授 (30270375)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAGUCHI Akemi 有明工業高等専門学校, 技術支援センター, 専門職員 (90399262)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 発生影響評価法 / パルスパワー / メダカ / DNAマイクロアレイ / タンパク合成阻害剤 / トランスクリプトーム / フェノーム |
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| Research Abstract |
We developed a novel method of chemical developmental toxicity assay using pulsed power electroporation chemical transfer technique for fertilized medaka egg. We could find the best pulsed power treatment condition which introduced chemicals efficiently and did not affected embryogenesis and gene expression. Then we measured uptake of bisphenol A under this condition by GC-MS. Bisphenol A concentration in egg extract was higher than immersed solution. According to DNA microarray data, we found the specific gene expression profile by uptake of bisphenol A.
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