2012 Fiscal Year Final Research Report
Creation of a self-organizing protein nano-structures using bacterial micro compartments
| Project/Area Number |
23651106
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
YOHDA Masafumi 東京農工大学, 大学院・工学研究院, 教授 (50250105)
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| Co-Investigator(Renkei-kenkyūsha) |
ODAKA Masafumi 東京農工大学, 大学院・工学研究院, 准教授 (20224248)
NOGUCHI Keiichi 東京農工大学, 学術研究支援総合センター, 准教授 (00251588)
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| Project Period (FY) |
2011 – 2012
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| Keywords | バクテリアマイクロコンパートメント / ナノバイオテクノロジー / ナノ構造体 / 超分子構造 |
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| Research Abstract |
Formation of bacterial microcompartment like nano-particle was observed by expressing 5 different shell proteins from ethanolamin-utilizing microcompartment in Escherichia coli. Expression, purification, crystal struture detemination and interaction study of shell proteins from carbosysome of a thermophilic cyanobacterium were performed. Expression and purification of encapsulin from Rhodococcous erythropolis N771 was performed.
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