Nucleic acid-metal-organic nanostructure based on nucleic aciddesignability and metal complex functionality

2012 Fiscal Year Final Research Report

• Project/Area Number: 23651130
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Konan University
• Principal Investigator: MIYOSHI Daisuke 甲南大学, フロンティアサイエンス学部, 准教授 (50388758)
• Project Period (FY): 2011 – 2012
• Keywords: DNAデバイス / DNA ナノテクノロジー / 金属有機構造体

Research Abstract

To achieve practical application of bottom-up nanotechnology, designability and functionality are required. Here, we used nucleic acids as designable nanostructure scaffolds and metal complexes as functional materials. Especially, to fabricate metal complexes on DNA nanostructures, we focused on G-quadruplex, which has large aromatic planner surface. It was found that long guanine-rich DNAs fold to form a beads-on-a-string structure, making us possible to design a G-quadruplex array. Moreover, some anionic ligands such as copper phthalocyanine are suitable to bind G-quadruplex specifically. These results allow us to design nucleic acid-metal-organic nanostructure based on nucleic acid designability and metal complex functionality.

Research Products

• [Journal Article] SugimotoStudy on Effects of Molecular Crowding on G-quadruplex-ligand Binding and Ligand-mediated Telomerase InhibitionMethods (2013)
  • Author(s): H. Yaku, T. Murashima, H. Tateishi-Karimata, S. Nakano, D. Miyoshi, N.
  • Volume: (in press)
  • Peer Reviewed
• [Journal Article] Thermodynamics - Hydration Relationshipswithin Loops that Affect G-Quadruplexes underMolecular Crowding Conditions (2013)
  • Author(s): T. Fujimoto, S. Nakano, N. Sugimoto, D.Miyoshi
  • Journal Title: J. Phys. Chem. B Volume: 117 Pages: 963-972
  • Peer Reviewed
• [Journal Article] The beads-on-a-string structure of long telomeric DNAs under molecular crowding conditions (2012)
  • Author(s): H. Yu, X. Gu, S. Nakano, D. Miyoshi, N.Sugimoto
  • Journal Title: J. Am. Chem. Soc. Volume: 134 Pages: 20060-20069
  • Peer Reviewed
• [Journal Article] Miyoshi, Naoki Sugimoto Phthalocyanines: a new class ofG-quadruplex-ligands with many potentialapplications (2012)
  • Author(s): H. Yaku, T. Fuj imoto, T. Murashima, D.
  • Journal Title: Chem. Commun. Volume: 48 Pages: 6203-6216
  • Peer Reviewed
• [Journal Article] SugimotoEffect of Locked Nucleic Acid Modification on Thermal Stability of Non-canonical DNA Structure (2011)
  • Author(s): J. Bhattacharyya, S. Maiti, S. Muhuri, S. Nakano, D. Miyoshi, N.
  • Journal Title: Biochemistry Volume: 50 Pages: 7414-7425
  • Peer Reviewed
• [Presentation] Ta-kashi Murashima, Naoki Sugimoto (2012)
  • Author(s): Daisuke Miyoshi, Valerie Gabelica, RyuichiMaeda, Takeshi Fujimoto, Hidenobu Yaku
  • Organizer: THIOFLAVINE-T AS HUMAN TELOMERIC G-QUADRUPLEX PROBEXX International Roundtable on Nucleosides, Nucleotides and Nucleic Acids
  • Place of Presentation: Montreal, Canada
  • Year and Date: 20120806-09
• [Presentation] 細胞内環境因子に応答する核酸デバイスの開発 (2012)
  • Author(s): 三好大輔
  • Organizer: 日本化学会第92春季年会(若い世代の特別講演会)
  • Place of Presentation: 東京
  • Year and Date: 2012-03-27
• [Presentation] TakeshiFujimoto, Takashi Murashima, Naoki SugimotoA new fluorescent light-up probe for DNAThe (2011)
  • Author(s): Daisuke Miyoshi, Hidenobu Yaku
  • Organizer: 38th International Sympoisum on NucleicAcid Chemistry
  • Place of Presentation: Hokkaido Univ
  • Year and Date: 2011-11-10
• [Book] Molecular Crowding and Hydration Regulatingof G-Quadruplex Formation (2013)
  • Author(s): D. Miyoshi, T. Fujimoto, N. Sugimoto
  • Total Pages: 330,87-110
  • Publisher: Topics in Current Chemistry
• [Book] G-quartet, G-quadruplex, and G-wire Regulatedby Chemical Stimuli (2011)
  • Author(s): D. Miyoshi, N.Sugimoto
  • Total Pages: 93-104
  • Publisher: DNA Nanotechnology (Methods in MolecularBiology series), 749
• [Remarks]
  • URL: http://www.pi.konan-u.ac.jp/miyoshi/index.html