2013 Fiscal Year Final Research Report
Development of high-performance microchip for DNA amplification towards sigle-cell genome analysis
| Project/Area Number |
23651131
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
MATSUURA SHUN-ICHI 独立行政法人産業技術総合研究所, コンパクト化学システム研究センター, 主任研究員 (80443224)
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| Co-Investigator(Kenkyū-buntansha) |
BABA Tomoya 大学共同利用機関法人情報・システム研究機構・新領域融合研究センター, 特任准教授 (00338196)
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| Project Period (FY) |
2011 – 2013
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| Keywords | DNAポリメラーゼ / メソポーラスシリカ / 固定化酵素 / DNA増幅 / PCR |
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| Research Abstract |
We successfully immobilized thermostable DNA polymerase on seven types of mesoporous silicas with different pore diameters and retained enzymatic activity sufficient for amplifying target DNA. The mesoporous silica support enabled the simple, selective, and preferential immobilization of DNA polymerase and the regulation of enzymatic activity for DNA amplification from small amounts of substrate DNA by optimizing pore size. In addition, the microfluidic chip containing composites of the enzyme and mesoporous silica exhibited precise, efficient, and continuous amplification of target DNA. Thus, these data show the great potential of mesoporous silica as enzymatic reaction platforms not only for conventional DNA amplification but also for efficient DNA amplification from small amounts of sample such as several molecules of DNA.
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