2011 Fiscal Year Final Research Report
A Genome-wide screening of circadian transcripts using a novel poly(A) determination method
| Project/Area Number |
23651184
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | Kanazawa University |
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Principal Investigator |
TEI Hajime 金沢大学, 自然システム学系, 教授 (00242115)
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| Project Period (FY) |
2011
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| Keywords | 概日リズム / 時計遺伝子 / 視交叉上核 / Periodl / poly(A) / 翻訳制御 |
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| Research Abstract |
We have developed a novel method to determine the length of poly(A) in eukaryotic mRNA, and designated as PACHINCO(Poly(A) Capture by Hairpin Chimeric Oligonucleotide)-RT-PCR. In this year, we optimized the application of the method to an automated microchip electrophoresis system(MultiNA, SHIMADZU), including the development of the distribution analysis algorithm of a variety of poly(A) length.
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Research Products
(1 results)
