A novel study to identify DNA determinants that generate the meiotic recombination hotspots

2013 Fiscal Year Final Research Report

• Project/Area Number: 23651185
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: Waseda University
• Principal Investigator: OHYAMA Takashi 早稲田大学, 教育・総合科学学術院, 教授 (60268513)
• Project Period (FY): 2011 – 2012
• Keywords: 減数分裂期組換え / 高頻度組換え部位 / DSBホットスポット / 相同組換え / 出芽酵母ゲノム / 分裂酵母ゲノム / マウスゲノム / 二本鎖DNA崩壊エネルギー

Research Abstract

We explored characteristics of the physical properties and conformations of DSB (double-strand break) hotspots on the genomes of yeasts (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and mouse (Mus musculus). When averaged, DSB hotspots of each organism clearly showed a distinctive mechanical property compared to the surrounding regions. However, very interestingly, the properties themselves were opposite between the yeasts and mouse: i.e., DSB hotspots are very rigid in the former while very flexible in the latter. Another physical property to be noted was that DSB hotspots of all the three organisms show distinctively high duplex disruption energy compared to the surrounding regions.

Research Products

• [Journal Article] Regions with unusually high flexibility occur frequently in human genomic DNA (2013)
  • Author(s): Kimura, H., Kageyama, D., Furuya, M., Sugiyama, S., Murata, N. and Ohyama, T
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 77 Pages: 612-617
  • DOI: 10.1271/bbb.120850
  • Peer Reviewed
• [Journal Article] The genome folding mechanism in yeast (2013)
  • Author(s): Kimura, H., Shimooka, Y., Nishikawa, J., Miura, O., Sugiyama, S., Yamada, S. and Ohyama, T
  • Journal Title: J. Biochemistry Volume: 154 Pages: 137-147
  • DOI: 10.1093/jb/mvt033
  • Peer Reviewed
• [Presentation] 減数分裂期高頻度組換え部位に特徴的なDNA の物理的特性 (2013)
  • Author(s): 三浦 理, 大懸 俊廣, 大山隆
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 20131200
• [Presentation] Meiotic recombination hotspots have distinctive properties of DNA (2013)
  • Author(s): Osamu Miura, Toshihiro Ogake and Takashi Ohyama
  • Organizer: The 2013 ASCB annual meeting
  • Place of Presentation: New Orleans
  • Year and Date: 20131200
• [Presentation] DNA properties that are distinctive of meiotic recombination hotspots (2013)
  • Author(s): Osamu Miura, Toshihiro Ogake and Takashi Ohyama
  • Organizer: 23rd Wilhelm Bernhard Workshop on the cell nucleus
  • Place of Presentation: Debrecen
  • Year and Date: 20130800
• [Presentation] Self-organizing and self-assembling properties of chromatin fiber (2013)
  • Author(s): Takashi Ohyama
  • Organizer: 1st Singapore-Japan-India Joint Symposium on Protein-DNA interactions in prokaryotic nucleoid and eukaryotic chromatin
  • Place of Presentation: Singapore
  • Year and Date: 20130700
  • Invited
• [Presentation] Characteristics of DNA in meiotic recombination hotspots (2012)
  • Author(s): Osamu Miura, Hajime Kimura, Toshihiro Ogake and Takashi Ohyama
  • Organizer: 2012 Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: San Francisco
  • Year and Date: 20121200
• [Presentation] 特殊 DNA 構造とヌクレオソーム配置の関係 : 各種生物を用いたゲノムワイドな解析 (2012)
  • Author(s): 大懸俊廣, 三浦理, 木村元, 大山隆
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 20121200
• [Presentation] 減数分裂期組換えホットスポットの DNA 特性 (2012)
  • Author(s): 三浦理, 木村元, 大懸俊廣, 大山隆
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 20121200
• [Presentation] 特殊 DNA 構造のゲノム内分布とヌクレオソーム配置 (2012)
  • Author(s): 大懸俊廣, 三浦理, 木村元, 大山隆
  • Organizer: 平成 24年度日本生化学会関東支部例会
  • Place of Presentation: 群馬
  • Year and Date: 20120600
• [Presentation] 高頻度組換え部位形成の情報基盤 : DNA 物性から謎を解く (2011)
  • Author(s): 三浦理, 木村元, 大懸俊廣, 大山隆
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20111200