2013 Fiscal Year Final Research Report
Analysis of genome inactivation mechanism using chromosomal site-specific gene integration
| Project/Area Number |
23651189
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
ABE KUNIYA 独立行政法人理化学研究所, バイオリソースセンター, チームリーダー (40240915)
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| Co-Investigator(Renkei-kenkyūsha) |
ARAKI Araki 熊本大学, 生命資源研究・支援センター, 准教授 (90211705)
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| Project Period (FY) |
2011 – 2012
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| Keywords | X染色体不活性化 / 偽常染色体 / エピジェネティクス / 遺伝子導入技術 |
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| Research Abstract |
In this study, we first devised a site-specific gene integration method, and established two female mouse lines, whose two X chromosomes carry different fluorescent protein genes. X chromosome inactivation phenomenon can be visualized using these mouse lines. Processes of X inactivation and reactivation can be analyzed in living cells of these mouse lines for the first time.
Using female somatic cells isolated from hybrid individuals between Xist knockout mice and asian mouse subspecies, ChIP-Seq analysis of histone modifications has been conducted. Differences in epigenetic states between inactive X and active X has been revealed.
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Research Products
(17 results)
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[Journal Article] RNAi-mediated knockdown of Xist does not rescue the impaired development of female cloned mouse embryos2013
Author(s)
Oikawa M, Matoba S, Inoue K, Kamimura S, Hirose M, Ogonuki N, Shiura H, Sugimoto M, Abe K, Ishino F, and Ogura A
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Journal Title
J. Repord. Dev
Volume: 59
Pages: 231-237
Peer Reviewed
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[Journal Article] Rev7 regulates function of DNA polymerase ζ in mouse development2013
Author(s)
Abbasi A, Khalaj M, Akiyama K, Yamanishi H, Kikuchi S, Hirose M, Yuzuriha M, Magari M, Degheidy HA, Abe K, Ogura A, Hashimoto H and Kunieda T
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Journal Title
J. Biol. Chem
Volume: 288
Pages: 10459-10471
DOI
Peer Reviewed
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[Journal Article] Epigenetic regulation of sex determination by the histone demethylase Jmjd1a2013
Author(s)
Kuroki S, Matoba S, Akiyoshi M, Matsumura Y, Miyachi H, Mise N, Abe K, Ogura A, Wilhelm D, Koopman P, Nozaki M, Kanai Y, Shinkai Y, and Tachibana, M
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Journal Title
Science
Volume: 341
Pages: 1106-1109
DOI
Peer Reviewed
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[Journal Article] Cell-cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells2012
Author(s)
Okamura D, Maeda I, Taniguchi H, Tokitake Y, Ikeda M, Ozato K, Mise N, Abe K, Noce T, Belmonte JCI, and Matsui Y
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Journal Title
Genes Dev
Volume: 26
Pages: 2477-2482
DOI
Peer Reviewed
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[Journal Article] Molecular identification of t^<w5> : Vps52 promotes pluripotential cell differentiation through cell-cell interactions2012
Author(s)
Sugimoto M, Kondo M, Hirose M, Suzuki M, Mekada K, Abe T, Kiyonari H, Ogura A, Takagi N, Artzt K, and Abe K
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Journal Title
Cell Reports
Volume: 2
Pages: 1363-1374
DOI
Peer Reviewed
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[Journal Article] The ectopic expression of Ptf1a induces spinal defects, urogenital defects and anorectal malformations in Danforth's short tail mice2012
Author(s)
Semba K, Araki K, Matsumoto K-I, Suda H, Ando T Sei A, Mizuta H, Takagi K, Nakahara M, Muta M, Yamada G, Nakagata N, Iida A, Ikegawa Y, Araki M, Abe K, and Yamamura K
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Journal Title
PLoS Genet
Volume: 9
Pages: e1003204
DOI
Peer Reviewed
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[Journal Article] RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos2011
Author(s)
Matoba S, Inoue K, Kohda T, Sugimoto M, Mizutani E, Ogonuki N, Nakamura T, Abe K, Nakano T, Ishino F, and Ogura A
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Journal Title
Proc. Natn. Acad Sci USA
Volume: 108
Pages: 20621-20626
DOI
Peer Reviewed
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